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KPNB1 (NM_002265) Human Untagged Clone
CAT＃: SC118719
KPNB1 (untagged)-Human karyopherin (importin) beta 1 (KPNB1)
CNY 9,632.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IMB1; Impnb; IPO1; IPOB; NTF97 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002265 edited
CCAACCCCCATCCCCAGTTCGAGCCGCCGCCCGAAAGGCCGGGCCGTCGTCTTAGGAGGA GTCGCCGCCGCCGCCACCTCCGCCATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCT CCCGATCGGCTGGAGCTGGAAGCGGCGCAGAAGTTCCTGGAGCGTGCGGCCGTGGAGAAC CTGCCCACTTTCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCCAGGAAACAGTCAGGTT GCCAGAGTTGCAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCAGATATC AAGGCACAATATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAG AACTATGTTTTGCAGACATTGGGTACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGT GTGGCTGGTATTGCTTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAG CTGGTGGCCAATGTCACAAACCCCAACAGCACAGAGCACATGAAGGAGTCGACATTGGAA GCCATCGGTTATATTTGCCAAGATATAGACCCAGAGCAGCTACAAGATAAATCCAATGAG ATTCTGACTGCCATAATCCAGGGGATGAGGAAAGAAGAGCCTAGTAATAATGTGAAGCTA GCTGCTACGAATGCACTCCTGAACTCATTGGAGTTCACCAAAGCAAACTTTGATAAAGAG TCTGAAAGGCACTTTATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGG GTACGAGTGGCTGCTTTACAGAATCTGGTGAAGATAATGTCCTTATATTATCAGTACATG GAGACATATATGGGTCCTGCTCTTTTTGCAATCACAATCGAAGCAATGAAAAGTGACATT GATGAGGTGGCTTTACAAGGGATAGAATTCTGGTCCAATGTCTGTGATGAGGAAATGGAT TTGGCCATTGAAGCTTCAGAGGCAGCAGAACAAGGACGGCCCCCTGAGCACACCAGCAAG TTTTATGCGAAGGGAGCACTACAGTATCTGGTTCCAATCCTCACACAGACACTAACTAAA CAGGACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTGTGCCTC ATGCTTCTGGCCACCTGCTGTGAAGATGACATTGTCCCACATGTCCTCCCCTTCATTAAA GAACACATCAAGAACCCAGATTGGCGGTACCGGGATGCAGCAGTGATGGCTTTTGGTTGT ATCTTGGAAGGACCAGAGCCCAGTCAGCTCAAACCACTAGTTATACAGGCTATGCCCACC CTAATAGAATTAATGAAAGACCCCAGTGTAGTTGTTCGAGATACAGCTGCATGGACTGTA GGCAGAATTTGTGAGCTGCTTCCTGAAGCTGCCATCAATGATGTCTACTTGGCTCCCCTG CTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGCTTCAAATGTGTGCTGG GCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAGCTGCAGACGTTGCTGATGATCAGGAA GAACCAGCTACTTACTGCTTATCTTCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAG ACTACAGACAGACCTGATGGACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTG ATGGAAATTGTGAAAAACAGTGCCAAGGATTGTTATCCTGCTGTCCAGAAAACGACTTTG GTCATCATGGAACGACTGCAACAGGTTCTTCAGATGGAGTCACATATCCAGAGCACATCC GATAGAATCCAGTTCAATGACCTTCAGTCTTTACTCTGTGCAACTCTTCAGAATGTTCTT CGGAAAGTGCAACATCAAGATGCTTTGCAGATCTCTGATGTGGTTATGGCCTCCCTGTTA AGGATGTTCCAAAGCACAGCTGGGTCTGGGGGAGTACAAGAGGATGCCCTGATGGCAGTT AGCACACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTTAAACCC TTCCTGGGCATTGGATTAAAAAATTATGCTGAATACCAGGTTTGTTTGGCAGCTGTGGGC TTAGTGGGAGACTTGTGCCGTGCCCTGCAATCCAACATCATACCTTTCTGTGACGAGGTG ATGCAGCTGCTTCTGGAAAATTTGGGGAATGAGAACGTCCACAGGTCTGTGAAGCCGCAG ATTCTGTCAGTGTTTGGTGATATTGCCCTTGCTATTGGAGGAGAGTTTAAAAAATACTTA GAGGTTGTATTGAATACTCTTCAGCAGGCCTCCCAAGCCCAGGTGGACAAGTCAGACTAT GACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTGGAAGCCTATACTGGAATC GTCCAGGGATTAAAGGGGGATCAGGAGAACGTACACCCGGATGTGATGCTGGTACAACCC AGAGTAGAATTTATTCTGTCTTTCATTGACCACATTGCTGGAGATGAGGATCACACAGAT GGAGTAGTAGCTTGTGCTGCTGGACTAATAGGGGACTTATGTACAGCATTTGGGAAGGAT GTACTGAAATTAGTAGAAGCTAGGCCAATGATCCATGAATTGTTAACTGAAGGGCGGAGA TCGAAGACTAACAAAGCAAAAACCCTTGCTACATGGGCAACAAAAGAACTGAGGAAACTG AAGAACCAAGCTTGATCTGTTACCATTGGGATGATAACCTGAGGACCCCCACTGGAAATC TCCCATCTTTTGAAAAACCTGGAAGTGAGGAGTGTGCACGGATGCTGAATGTTTGGGAAT GAGAGGATGAGTGAGTGAGGCTTGAAAACACACCACATTGAAAATCCTGCCACAGCAGCA GCCGCAGCCGCCAACAGCAGCGCTGTTAGTGAGCTAAGTAAGCACTGACTTCGTAGAAAA CCATAACATCGGCCATCTTGGAAAAGAGAAAAACAATGGAGTTACTTATTTAAAAAAAAA >OriGene 5' read for NM_002265 unedited
GGGCACTATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCAACCCCC ATCCCCAGTTCGAGCCGCCGCCCGAAGGCCGGGCCGTCGTCTTAGGAGGAGTCGCCGCCG CCGCCACCTCCGCCATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCTCCCGATCGGC TGGAGCTGGAAGCGGCGCAGAAGTTCCTGGAGCGTGCGGCCGTGGAGAACCTGCCCACTT TCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCCAGGAAACAGTCAGGTTGCCAGAGTTG CAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCAGATATCAAGGCACAAT ATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAGAACTATGTTT TGCAGACATTGGGTACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGTGTGGCTGGTA TTGCTTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAGCTGGTGGCCA ATGTCACAAACCCCAACAGCACAGAGCACATGAAGGAGTCGACATTGGAAGCCATCGGTT ATATTTGCCAAGATATAGACCCAGAGCAGCTACAAGATAAATCCAATGAGATTCTGACTG CCATAATCCAGGGGATGAGGAAAGAAGAGCCTAGTAATAATGTGAAGCTAGCTGCTACGA ATGCACTCCTGAACTCATTGGAGTTCACCAAAGCANACTTTGNATAAGAGTCTGAAAGGC ACTNTATTATGCAGGTGGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGTACGAGTGG GCTGCTTACAGAATCTGGTGAAGNATATGTCCTTATATTATCAGTACATGGAGACATATA TGGGTCCTGCTCTT >OriGene 3' read for NM_002265 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATAAGTAACTCCATTGTTTTTC TCTTTTCCAAGATGGCCGATGTTATGGTTTTCTACGAAGTCAGTGCTTACTTATCTCACT AACAGCGCTGCTGTTGGCGGCTGCGGCTGCTGCTGTGGCAGGATTTTCAATGTGGTGTGT TTTCAAGCCTCACTCACTCATCCTCTCATTCCCAAACATTCAGCATCCGTGCACACTCCT CACTTCCAGGTTTTTCAAAAGATGGGAGATTTCCAGTGGGGGTCCTCAGGTTATCATCCC AATGGTAACAGATCAAGCTTGGTTCTTCAGTTTCCTCAGTTCTTTTGTTGCCCATGTAGC AAGGGTTTTTGCTTTGTTAGTCTTCGATCTCCGCCCTTCAGTTAACAATTCATGGATCAT TGGCCTAGCTTCTACTAATTTCAGTACATCCTTCCCAAATGCTGTACATAAGTCCCCTAT TAGTCCAGCAGCACAAGCTACTACTCCATCTGTGTGATCCTCATCTCCAGCAATGTGGTC AATGAAAGACAGAATAAATTCTACTCTGGGTTGTACCAGCATCACATCCGGGTGTACGTT CTCCTGATCCCCCTTTAATCCCTGGACGATTCCAGTATATGCCTCCAAGCAGCTNTCCCT TAGCTCATTCAGATAATCCACCATGTCATAGTCTGACTTGTCCACCTGGGCTAGGGAAGC CCGCTGAAAATTATTCAATACAACCCTCTAGTATTCTTTAACTCTCCTCAATAGCAAGGG CATCTCACCAAACCCTGACAGAATCTGCGGCTTCACAGACCTGTGGACGTTCTCATTCCC CAATTTTCCAAAGCCACTGCTCACCCCTGCCAAAAAGGTTAATGTTGATCGCAAGCCACG CCCAATCTTCCACTAACCCCAGCTTGCAAACAAACCGGTATTCAATATATTTTTATTCCA TGCCACGAAGGGTTAAGACTCCCTGACTCGAGAATTCCCCCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002265 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002265.4, NP_002256.2 |
| RefSeq Size | 4205 bp |
| RefSeq ORF | 2631 bp |
| Locus ID | 3837 |
| UniProt ID | Q14974 |
| Domains | Armadillo_seg, IBN_NT |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Gene Summary | Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200659 | KPNB1 (Myc-DDK-tagged)-Human karyopherin (importin) beta 1 (KPNB1) |
CNY 9,624.00 |
|
| RC200659L1 | Lenti ORF clone of Human karyopherin (importin) beta 1 (KPNB1), Myc-DDK-tagged |
CNY 12,024.00 |
|
| RC200659L2 | Lenti ORF clone of Human karyopherin (importin) beta 1 (KPNB1), mGFP tagged |
CNY 9,410.00 |
|
| RC200659L3 | Lenti ORF clone of Human karyopherin (importin) beta 1 (KPNB1), Myc-DDK-tagged |
CNY 12,024.00 |
|
| RC200659L4 | Lenti ORF clone of Human karyopherin (importin) beta 1 (KPNB1), mGFP tagged |
CNY 12,024.00 |
|
| RG200659 | KPNB1 (tGFP-tagged) - Human karyopherin (importin) beta 1 (KPNB1) |
CNY 8,270.00 |
