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GAPDH (NM_002046) Human Untagged Clone
CAT＃: SC118869
GAPDH (untagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
CNY 3,656.00
| Cited in 10 publications. |
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | G3PD; GAPD; HEL-S-162eP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002046 edited
ATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCACCAGG GCTGCTTTTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTGACCTC AACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCACCGTC AAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGGAGCGA GATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGC GTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGGTCATC ATCTCTGCCCCCTCTGCTGACGCCCCCATGTTCGTCATGGGTGTGAACCATGAGAAGTAT GACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAGCACCCCTG GCCAAGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCC ATCACTGCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGC GGGGCTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTC ATCCCTGAGCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTG TCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGAAG GTGGTGAAGCAGGCGTCGGAGGGCCCCCTCAAGGGCATCCTGGGCTACACTGAGCACCAG GTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTCCACCTTCGACGCTGGGGCTGGC ATTGCCCTCAACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAACGAATTTGGCTAC AGCAACAGGGTGGTGGACCTCATGGCCCACATGGCCTCCAAGGAGTAA >OriGene 5' read for NM_002046 unedited
ACGAGGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTGAN ACACCATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCA CCAGGGCTGCTTTTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTG ACCTCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCA CCGTCAAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGG AGCGAGATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCA CTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGG TCATCATCTCTGCCCCCTCTGCTGACGCCCCCATGTTCGTCATGGGTGTGAACCATGAGA AGTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAACAC CCCTGGCCAAGGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTC CATGCCATCACTGCCACCCAAAANACTGTGGGATGGCCCCCTNCGGGAAACTGTGGGCGT GATGGCCCGNGGGGCTCTNCANAACATTATNCCTGCCTNTACTGGGCGCTGCCAAGGCTG GGGGGCAAGGTCATTCCTTGAACTGAACGGGAANCTCACTGGCATGGGCCTTTCGTGTCC CCCTGGCAACGTGTTAGGGGGGGGACCTGACCTGCCGTTAAAAAAANCTGCCAAATTGAT GACCTCAAAAAGGGG >OriGene 3' read for NM_002046 unedited
TTGGTATGGCAACTTCCAGGNCCAGGTANAGCACTGGGGNAGGGTCACAGGNATGCCACC CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTT TTTTTTTTTGGTTGAGCACAGGGTACTTTATTGATGGTACATGACAAGGTGCGGCTCCCT AGGCCCCTCCCCTCTTCAAGGGGTCTACATGGAAACTGTGAGGAGGGGAGATTCAGTGTG GTGGGGGACTGAGTGTGGCAGGGACTCCCCAGCAGTGAGGGTCTCTCTCTTCCTCTTGTG CTCTTGCTGGGGCTGGTGGTCCAGGGGTCTTACTCCTTGGAGGCCATGTGGGCCATGAGG TCCACCACCCTGTTGCTGTAGCCAAATTCGTTGTCATACCAGGAAATGAGCTTGACAAAG TGGTCGTTGAGGGCAATGCCAGCCCCAGCGTCNAAGGTGGAGGAGTGGGTGTCGCTGTTG AAGTCAGAGGAGACCACCTGGTGCTCAGTGTANCCCAGGATGCCCTTGAGGGGGCCCTCC GACGCCTGCTTCACCACCTTCTTGATGTCATCATATTTGGCAGGTTTTTCTAGACGGCAG GTCAGGTCCACCACTGACACGTTGGCAGTGGGGACACGGAAGGCCATGCCAGTGAGCTTC CCGTTCAGCTCAGGGATGACCTTGCCCACAGCCTTGGCAGCGCCAGTAAAGGCAGGGATG ATGTTCTGGAGACCCCGCNGNCATCACGCACAGTTTCCCGGAGGGGGCATCCACAGTCTT CTGGGTGCANNGATGGCATGGACCTGTGTCATGATTCCTTCACGATACCCAAAGTGCATG GATGACCTGGNCAGGGTGCTAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002046 |
| Insert Size | 1008 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002046.2, NP_002037.2 |
| RefSeq Size | 1283 bp |
| RefSeq ORF | 1008 bp |
| Locus ID | 2597 |
| UniProt ID | P04406 |
| Domains | gpdh |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
| Gene Summary | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Citations (10)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Characterization of the Cardiac Overexpression of HSPB2 Reveals Mitochondrial and Myogenic Roles Supported by a Cardiac HspB2 Interactome
,null,
PLoS ONE
,PubMed ID 26465331
[GAPDH]
|
|
Identification of the Binding Partners for HspB2and CryAB Reveals Myofibril and MitochondrialProtein Interactions and Non-Redundant Roles forSmall Heat Shock Proteins
,Langston, KM;,
Thesis
[GAPDH]
|
|
Human and Pneumococcal Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Proteins Are Both Ligands of Human C1q Protein
,Rémi Terrasse, Pascale Tacnet-Delorme, Christine Moriscot, Julien Pérard, Guy Schoehn, Thierry Vernet, Nicole M. Thielens, Anne Marie Di Guilmi, and Philippe Frachet,
J. Biol. Chem., Dec 2012; 287: 42620 - 42633.
[GAPDH]
|
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Expression of Organic Anion Transporter 2 in the Human Kidney and Its Potential Role in the Tubular Secretion of Guanine-Containing Antiviral Drugs
,Yaofeng Cheng, Arpine Vapurcuyan, Mohammad Shahidullah, Lauren M. Aleksunes, and Ryan M. Pelis,
Drug Metab. Dispos., Mar 2012; 40: 617 - 624.
[GAPDH]
|
|
Non-productive HIV-1 infection of human glomerular and urinary podocytes
,null,
Virology
,PubMed ID 20937511
[GAPDH]
|
|
Inhibition of Encephalomyocarditis Virus and Poliovirus Replication by Quinacrine: Implications for the Design and Discovery of Novel Antiviral Drugs
,Alexander V. Gasparian, Nickolay Neznanov, Sujata Jha, Oleksandr Galkin, John J. Moran, Andrei V. Gudkov, Katerina V. Gurova, and Anton A. Komar,
J. Virol., Sep 2010; 84: 9390 - 9397
[GAPDH]
|
|
Inhibition of Encephalomyocarditis Virus and Poliovirus Replication by Quinacrine: Implications for the Design and Discovery of Novel Antiviral Drugs ▿
,null,
Journal of Virology
,PubMed ID 20631142
[GAPDH]
|
|
Inhibition of EMCV and poliovirus replication by quinacrine: implications for the design and discovery of novel anti-viral drugs
,Alexander V. Gasparian, Nickolay Neznanov, Sujata Jha, Oleksandr Galkin, John J. Moran, Andrei V. Gudkov, Katerina V. Gurova, and Anton A. Komar,
J. Virol., Jul 2010; 10.1128/JVI.02569-09
[GAPDH]
|
|
Ribozyme-mediated reduction of wild-type and mutant cartilage oligomeric matrix protein (COMP) mRNA and protein
,Joseph L. Alcorn, Thomas M. Merritt, Mary C. Farach-Carson, Huiqui H. Wang, and Jacqueline T. Hecht,
RNA, Apr 2009; 15: 686 - 695.
[GAPDH]
|
|
BMP-2/4 and BMP-6/7 differentially utilize cell surface receptors to induce osteoblastic differentiation of human bone marrow derived mesenchymal stem cells
,Karen S. Lavery, Pamela M. Swain, Dean Falb, and Moulay Hicham Alaoui-Ismaili,
J. Biol. Chem., Apr 2008; 10.1074/jbc.M800850200
[GAPDH]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202309 | GAPDH (Myc-DDK-tagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
CNY 5,488.00 |
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| RC202309L1 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged |
CNY 7,888.00 |
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| RC202309L2 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged |
CNY 7,888.00 |
|
| RC202309L3 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202309L4 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged |
CNY 7,888.00 |
|
| RG202309 | GAPDH (tGFP-tagged) - Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
CNY 7,088.00 |
