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ENO3 (NM_001976) Human Untagged Clone
CAT＃: SC118914
ENO3 (untagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 1
CNY 5,488.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GSD13; MSE |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118914 sequence for NM_001976 edited (data generated by NextGen Sequencing)
ATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAGGGGCAACCCCACGGTG GAGGTGGACCTGCACACGGCCAAGGGCCGATTCCGAGCAGCTGTGCCCAGTGGGGCTTCC ACGGGTATCTATGAGGCTCTGGAACTAAGAGACGGAGACAAAGGCCGCTACCTGGGGAAA GGAGTCCTGAAGGCTGTGGAGAACATCAACAGTACTCTGGGCCCTGCTCTGCTGCAAAAG AAACTAAGCGTTGCGGATCAAGAAAAAGTTGACAAATTTATGATTGAGCTAGATGGGACC GAGAATAAGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTCCTTGGCCGTGTGTAAG GCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCAGATCTCGCTGGGAAC CCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGGGGGCTCCCATGCTGGA AACAAGCTGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGCCAGCTCCTTCAAGGAA GCCATGCGCATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTAT GGGAAGGATGCCACCAATGTGGGTGATGAAGGTGGCTTCGCACCCAACATCCTGGAGAAC AATGAGGCCCTGGAGCTGCTGAAGACGGCCATCCAGGCGGCTGGTTACCCAGACAAGGTG GTGATCGGCATGGATGTGGCAGCATCTGAGTTCTATCGCAATGGGAAGTACGATCTTGAC TTCAAGTCGCCTGATGATCCCGCACGGCACATCACTGGGGAGAAGCTCGGAGAGCTGTAT AAGAGCTTTATCAAGAACTATCCTGTGGTCTCCATCGAAGACCCCTTTGACCAGGATGAC TGGGCCACTTGGACCTCCTTCCTCTCGGGGGTGAACATCCAGATTGTGGGGGATGACTTG ACAGTCACCAACCCCAAGAGGATTGCCCAGGCCGTTGAGAAGAAGGCCTGCAACTGTCTG CTGCTGAAGGTCAACCAGATCGGCTCGGTGACCGAATCGATCCAGGCGTGCAAACTGGCT CAGTCTAATGGCTGGGGGGTGATGGTGAGCCACCGCTCTGGGGAGACTGAGGACACATTC ATTGCTGACCTTGTGGTGGGGCTCTGCACAGGACAGATCAAGACTGGCGCCCCCTGCCGC TCGGAGCGTCTGGCCAAATACAACCAACTCATGAGGATCGAGGAGGCTCTTGGGGACAAG GCAATCTTTGCTGGACGCAAGTTCCGTAACCCGAAGGCCAAGTGA Clone variation with respect to NM_001976.4 212 a=>g;254 t=>c >OriGene 5' read for NM_001976 unedited
TGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCTACACTCACTCACAC CTCCTGTCCTGCAGCCATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAG GGGCAACCCCACGGTGGAGGTGGACCTGCACACGGCCAAGGGCCGATTCCGAGCAGCTGT GCCCAGTGGGGCTTCCACGGGTATCTATGAGGCTCTGGAACTAAGAGACGGAGACAAAGG CCGCTACCTGGGGAAAGGAGTCCTGAAGGCTGTGGAGAACATCAACAGTACTCTGGGCCC TGCTCTGCTGCAAAAGAAACTAAGCGTTGCGGATCAAGAAAAAGTTGACAAATTTATGAT TGAGCTAGATGGGACCGAGAATAAGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTC CTTGGCCGTGTGTAAGGCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGC AGATCTCGCTGGGAACCCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGG GGGCTCCCATGCTGGAAACAAGCTGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGC CAGCTCCTTCAAGGAAGCCATGCGCATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGT CATCAAGGCCAAGTATGGGAAGGATGCCACCAATGTGGGTGATGAAGGTGGCTTCGCACC CAACATCCTGGAGAACATGAGGCCCTGNAGCTGCTGAAGACGGCCATCCAGGCGGCTGGT TACCCAGACAAGGTGGTGATNCGCATGGGATGTGCAGCATCTGAGTTCTATCGCAATGGG AGTACGATCTTGACTTCAGTCGCCTGATGATCCCGCACGCACATACTGGGGAGAGCTCGG AGACTGNATAGAGCTTATCAGAACATNCTGTGTC >OriGene 3' read for NM_001976 unedited
CTGGCCCAGTGTTNTATTTCAGGAAGCAGTCTGGNAGNACTGGGTCTGTCCAGTGGAGTC CTGGAGCCTCCAGCTTCTCACTTGGCCTTCGGGTTACGGAACTTGCGTCCAGCAAAGATT GCCTTGTCCCCAAGAGCCTCCTCGATCCTCATGAGTTGGTTGTATTTGGCCAGACGCTCC GAGCGGCAGGGGGCGCCAGTCTTGATCTGTCCTGTGCAGAGCCCCACCACAAGGTCAGCA ATGAATGTGTCCTCAGTCTCCCCAGAGCGGTGGCTCACCATCACCCCCCAGCCATTAGAC TGAGCCAGTTTGCACGCCTGGATCGATTCGGTCACCGAGCCGATCTGGTTGACCTTCAGC AGCAGACAGTTGCAGGCCTTCTTCTCAACGGCCTGGGCAATCCTCTTGGGGTTGGTGACT GTCAAGTCATCCCCCACAATCTGGATGTTCACCCCCGAGAGGAAGGAGGTCCAAGTGGCC CAGTCATCCTGGTCAAAGGGGTCTTCGATGGAGACCACANGATAGTTCTTGATAAAGCTC TTATACAGCTCTCCGAGCTTCTCCCCAGTGATGTGCCGTGCGGGATCATCAGGCGACTTG AAGTCAAGATCGTACTTNCCATTGCGATAGAACTCAGATGCTGCCACATCCATGCCGATC ACCACCTTGTCTGGGTAACCAGCCGCCTGGATGGCCGTCTCTAACAGCTCCAAGGCCTAA TTGGTTTCCAAGATGCTGGGGGCAAAACCCCCTTAATACCCACATTGGGGGCATCCCTCC CTACCTCGCCCTTGAGACCCCCCTGAGGGGGGGGAGTACCTTCGGGCCAATGCGAATGGG CTTCTTGCAAGACCTGCGCTCCACGGCGCAACCATCACCCCCGCTTGCCCGCTTGGCCCC AACTGGCCCCCCCTCTACACCAATGCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001976 |
| Insert Size | 1560 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001976.2, NP_001967.1 |
| RefSeq Size | 1494 bp |
| RefSeq ORF | 1305 bp |
| Locus ID | 2027 |
| UniProt ID | P13929 |
| Domains | enolase |
| Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation |
| Gene Summary | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same protein. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA
,Baleva, M;Gowher, A;Kamenski, P;Tarassov, I;Entelis, N;Masquida, B;,
Int J Mol Sci
,PubMed ID 25918939
[ENO3]
|
|
Identification of the Binding Partners for HspB2and CryAB Reveals Myofibril and MitochondrialProtein Interactions and Non-Redundant Roles forSmall Heat Shock Proteins
,Langston, KM;,
Thesis
[ENO3]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219257 | ENO3 (Myc-DDK-tagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 1 |
CNY 5,488.00 |
|
| RC219257L3 | Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219257L4 | Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG219257 | ENO3 (tGFP-tagged) - Human enolase 3 (beta, muscle) (ENO3), transcript variant 1 |
CNY 7,088.00 |
