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GADD45A (NM_001924) Human Untagged Clone
CAT＃: SC118947
GADD45A (untagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1
CNY 1,200.00
CNY 2,950.00
| Cited in 4 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DDIT1; GADD45 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001924 edited
GAATTCGGCACGAGGGGCTGGGAGGCAGCGGCCCAATTAGTGTCGTGCGGCCCGTGGCGA GGCGAGGTCCGGGGAGCGAGCGAGCAAGCAAGGCGGGAGGGGTGGCCGGAGCTGCGGCGG CTGGCACAGGAGGAGGAGCCCGGGCGGGCGAGGGGCGGCCGGAGAGCGCCAGGGCCTGAG CTGCCGGAGCGGCGCCTGTGAGTGAGTGCAGAAAGCAGGCGCCCGCGCGCTAGCCGTGGC AGGAGCAGCCCGCACGCCGCGCTCTCTCCCTGGGCGACCTGCAGTTTGCAATATGACTTT GGAGGAATTCTCGGCTGGAGAGCAGAAGACCGAAAGGATGGATAAGGTGGGGGATGCCCT GGAGGAAGTGCTCAGCAAAGCCCTGAGTCAGCGCACGATCACTGTCGGGGTGTACGAAGC GGCCAAGCTGCTCAACGTCGACCCCGATAACGTGGTGTTGTGCCTGCTGGCGGCGGACGA GGACGACGACAGAGATGTGGCTCTGCAGATCCACTTCACCCTGATCCAGGCGTTTTGCTG CGAGAACGACATCAACATCCTGCGCGTCAGCAACCCGGGCCGGCTGGCGGAGCTCCTGCT CTTGGAGACCGACGCTGGCCCCGCGGCGAGCGAGGGCGCCGAGCAGCCCCCGGACCTGCA CTGCGTGCTGGTGACGAATCCACATTCATCTCAATGGAAGGATCCTGCCTTAAGTCAACT TATTTGTTTTTGCCGGGAAAGTCGCTACATGGATCAATGGGTTCCAGTGATTAATCTCCC TGAACGGTGATGGCATCTGAATGAAAATAACTGAACCAAATTGCACTGAAGTTTTTGAAA TACCTTTGTAGTTACTCAAGCAGTTACTCCCTACACTGATGCAAGGATTACAGAAACTGA TGCCAAGGGGCTGAGTGAGTTCAACTACATGTTCTGGGGGCCCGGAGATAGATGACTTTG CAGATGGAAAGAGGTGAAAATGAAGAAGGAAGCTGTGTTGAAACAGAAAAATAAGTCAAA AGGAACAAAAATTACAAAGAACCATGCAGGAAGGAAAACTATGTATTAATTTAGAATGGT TGAGTTACATTAAAATAAACCAAATATGTTAAAGTTTAAGTGTGCAGCCATAGTTTGGGT ATTTTTGGTTTATATGCCCTCAAGTAAAAGAAAAGCCGAAAGGGTTAATCATATTTGAAA ACCATATTTTATTGTATTTTGATGAGATATTAAATTCTCAAAGTTTTATTATAAATTCTA CTAAGTTATTTTATGACATGAAAAGTTATTTATGCTATAAATTTTTTGAAACACAATACC TACAATAAACTGGTATGAATAATTGCAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_001924 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGGGAGGCAGCG GCCCAATTAGTGTCGTGCGGCCCGTGGCGAGGCGAGGTCCGGGGAGCGAGCGAGCAAGCA AGGCGGGAGGGGTGGCCGGAGCTGCGGCGGCTGGCACAGGAGGAGGAGCCCGGGCGGGCG AGGGGCGGCCGGAGAGCGCCAGGGCCTGAGCTGCCGGAGCGGCGCCTGTGAGTGAGTGCA GAAAGCAGGCGCCCGCGCGCTAGCCGTGGCAGGAGCAGCCCGCACGCCGCGCTCTCTCCC TGGGCGACCTGCAGTTTGCAATATGACTTTGGAGGAATTCTCGGCTGGAGAGCACAAGAC CGAAAGGATGGATAAGGTGGGGGATGCCCTGGAGGAAGTGCTCAGCAAAGCCCTGAGTCA GCGCACGATCACTGTCGGGGTGTACGAAGCGGCCAAGCTGCTCAACGTCGACCCCGATAA CGTGGTGTTGTGCCTGCTGGCGGCGGACGAGGACGACGACAGAGATGTGGCTCTGCAGAT CCACTTCACCCTGATCCAGGCGTTTTGCTGCGAGAACGACCATAAACATCCTGCGCGTCA GCAACCCGGGGCCGGCTGGCGGAGCTCCTGCTCTAGAAGACCCGACGCTGGCCCCCGCGG CGAGCGAAGGGCGCCGAGCAGCCCCCGGACCTGCCCTGCGTGCTGGTGACGAATCCCCAT TTCTCCTCATGGAAGGGATCCTCGCCTTAGTGCACCTTATTTGTTTTTTGCCGGGAAAGT TCGTTCCATGGACAATTGGGGCCCCAGGGATTATTCNTCCCTGGACCGGGGAGGCCATTT GGAAGGAAAATACCTGACCCACATTGCCCGGAGGTTTTTGGAAAACCTTTGGAAGTTACT TAACCCGTTCCTCCCTTCCCTGTAGGCAAGGATAACAGTAACC >OriGene 3' read for NM_001924 unedited
TCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCAATTATTCATACCAG TTTATTGTAGGTATTGTGTTTCAAAAAATTATAGCATAAATAACTTTTCATGTCATAAAA TAACTTAGTAGAATTTATAATAAAACTTTGAGAATTTAATATCTCATCAAAATACAATAA AATATGGTTTTCAAATATGATTAACCCTTTCGGCTTTTCTTTTACTTGAGGGCATATAAA CCAAAAATACCCAAACTATGGCTGCACACTTAAACTTTAACATATTTGGTTTATTTTAAT GTAACTCAACCATTCTAAATTAATACATAGTTTTCCTTCCTGCATGGTTCTTTGTAATTT TTGTTCCTTTTGACTTATTTTTCTGTTTCAACACAGCTTCCTTCTTCATTTTCACCTCTT TCCATCTGCAAAGTCATCTATCTCCGGGCCCCCAGAACATGTAGTTGAACTCACTCAGCC CCTTGGCATCAGTTTCTGTAATCCTTGCATCAGTGTAGGGAGTAACTGCTTGAGTAACTA CAAAGGTATTTCAAAAACTTCAGTGCAATTTGGCTCAGTTATTTTCATTCAGATGCCATC ACCGTTCAGGGAGATTAATCACTGGAACCCATTGATCCATGTAGCGACTTTCCCGGCAAA AACCAATAAGTTGACTTAAGGCAGGATCCTTTCATTGAGATGAATGTGGATTCGTCACCA GCACGCAATGCAGGTCCCGGGGGCTGCTTCGCCGCCTCGCTCGNCGCGGGGTCAGCGGTG GTCTCCAAGACAAGAGCTCCGCCGCCGGCCCGGTTGCTGACGCGCAGAAGTTCATGCCCT TTTGCACAAAAGCCCGGATAAGGTGAGTGGTATTGCAAACCACATTTGTTGCGGCCTTGT CGCCGCCGCAGGACACACCCCGTATTGGGGCCACTTTAGCACTTGCCCGTTCGTCCCCCG AACGGGACGGCCGCCGACCAGGGTTTGCTGCCCTCCTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001924 |
| Insert Size | 1360 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001924.2, NP_001915.1 |
| RefSeq Size | 1355 bp |
| RefSeq ORF | 498 bp |
| Locus ID | 1647 |
| UniProt ID | P24522 |
| Domains | Ribosomal_L7Ae |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle, MAPK signaling pathway, p53 signaling pathway |
| Gene Summary | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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CArG-driven GADD45a activated by resveratrol inhibits lung cancer cells
,Shi, Q;Geldenhuys, W;Sutariya, V;Bishayee, A;Patel, I;Bhatia, D;,
Genes Cancer
,PubMed ID 26124921
[GADD45A]
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p53 controls hepatitis C virus non-structural protein 5A-mediated downregulation of GADD45a expression via the NF-B and PI3K–Akt pathways
,Du Cheng, Lei Zhao, Leiliang Zhang, Yongfang Jiang, Yi Tian, Xinqiang Xiao, and Guozhong Gong,
J. Gen. Virol., Feb 2013; 94: 326 - 335.
[GADD45A]
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Characterization of an alternatively spliced GADD45?, GADD45?1 isoform, in arsenic-treated epithelial cells
,null,
Molecular carcinogenesis
,PubMed ID 18942077
[GADD45A]
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Methylation-Mediated Repression of GADD45 in Prostate Cancer and Its Role as a Potential Therapeutic Target
,Kavitha Ramachandran, Gopal Gopisetty, Edna Gordian, Loida Navarro, Christiane Hader, Isildinha M. Reis, Wolfgang A. Schulz, and Rakesh Singal,
Cancer Res., Feb 2009; 69: 1527 - 1535.
[GADD45A]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204005 | GADD45A (Myc-DDK-tagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1 |
CNY 1,800.00 |
|
| RC204005L1 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC204005L2 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204005L3 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC204005L4 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged |
CNY 4,200.00 |
|
| RG204005 | GADD45A (tGFP-tagged) - Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1 |
CNY 3,400.00 |
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