ATP6V1B2 (NM_001693) Human Untagged Clone
CAT#: SC119083
ATP6V1B2 (untagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2)
CNY 7,088.00
Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ATP6B1B2; ATP6B2; DOOD; HO57; VATB; Vma2; VPP3; ZLS2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001693, the custom clone sequence may differ by one or more nucleotides
ATGGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCACCGGTGGGC CGGCGGTGGGAGCTCGGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTCCCAGCCTCGCCTCACATA CAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGATCATGTTAAGTTTCCCAGGTATGCTGAA ATTGTCCATTTGACCTTACCGGATGGCACAAAGAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGG CAGTAGTTCAGGTATTTGAAGGGACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGA TATTCTCCGAACACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGAC AGAGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCAATGTCGAA TCTACCCAGAGGAAATGATTCAGACTGGCATTTCGGCCATCGATGGGATGAACAGTATTGCTAGGGGGCA GAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGAGATTGCAGCTCAGATCTGTCGCCAGGCT GGTTTGGTAAAGAAATCCAAAGATGTAGTAGACTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTA TGGGTGTAAACATGGAAACTGCCCGGTTCTTCAAATCTGACTTTGAAGAAAATGGCTCAATGGACAATGT CTGCCTCTTTTTGAACTTGGCTAATGACCCAACCATTGAGCGAATTATCACTCCTCGCCTGGCTCTAACC ACAGCTGAATTTCTGGCGTACCAATGTGAGAAACATGTATTGGTTATTCTAACAGACATGAGTTCTTATG CTGAAGCACTTCGAGAGGTTTCAGCAGCCAGGGAAGAGGTACCTGGTCGACGAGGTTTTCCAGGTTACAT GTATACAGATTTAGCCACGATATATGAACGCGCTGGGCGAGTGGAAGGGAGAAACGGCTCGATTACTCAA ATCCCTATTCTAACCATGCCTAATGATGATATCACTCACCCCATCCCAGACTTGACTGGCTACATTACAG AGGGGCAGATCTATGTGGACAGACAGCTGCACAACAGACAGATTTATCCACCTATCAATGTGCTGCCCTC ACTATCACGGTTAATGAAGTCTGCTATTGGAGAAGGGATGACCAGGAAGGATCATGCCGATGTATCTAAC CAGCTATATGCGTGCTATGCTATTGGAAAGGATGTGCAAGCCATGAAAGCTGTCGTTGGAGAAGAAGCCC TTACCTCAGATGATCTTCTCTACTTGGAATTTCTGCAGAAGTTTGAGAGGAACTTCATTGCTCAGGGTCC TTACGAAAATCGCACTGTCTTTGAGACTTTGGACATTGGCTGGCAGCTACTCCGAATCTTCCCCAAAGAA ATGCTGAAGAGAATCCCTCAGAGCACCCTCAGCGAATTTTACCCTCGAGACTCTGCAAAGCATTAG >OriGene 5' read for NM_001693 unedited
TTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGACAGAGGAGACAAGAT GGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCAC CGGTGGGCCGGCGGTGGGAGCTCGGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTC CCAGCCTCGCCTCACATACAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGA TCATGTTAAGTTTCCCAGGTATGCTGAAATTGTCCATTTGACCTTACCGGATGGCACAAA GAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGGCAGTAGTTCAGGTATTTGAAGG GACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGATATTCTCCGAAC ACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGACAG AGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCA ATGTCGAATCTACCCAGAGGAAATGATTCAGACTGGCATTTCGGCCATCGATGGGATGAA CAGTATTGCTAGGGGGCAGAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGA GATTGCAGCTCAGATCTGTCGCCAGGCTGGTTTGGTAAAGAAATCCAAAGATGTAGTAGA CTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTATGGGGTGTAACATGGNNAACTG NCCNGTTCTTCANNATCTGACTTGAAGAAAATGGCTCATGGGACAATGTCTGCCTCTTTN TGAAACTGGGCTATGACCCACCATTGAGCGAATATCACTNCTCGNCTGGCTCTACCCAGT A >OriGene 3' read for NM_001693 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTAATTTAACATTTATTT TCATTTTATATCCAGATGTCACATTTTCTACATTAAAATATAATCATGCTGACTCCCCCA ACCCCCAGCCCTCAGAGAAAAAAGTGAAGAACTTTACACAACATGAAAGATGGAATGCAC GGGGTAGGGAAGTTGCTATTGCACCCAAGTAACAAAGAACACAGAGAGACCAACAGGACG CATGGCAGAACTGCATAGAAGATCGCTCAGGGCTCACGTCAGGAAGGCGCAGGAATTCAG AAAACTCTAAAGCTTGGAAATGCTATCGTTTGTGCCACAAAGATTACCCTGAAAAGGTAG AGAGAGGTCAAAAAGCAGAACCAAATTGGGGATTTTCAAAGGCTGCTCTCTAACAGAGTT TAAAATATCCGTAAACATAAGTGCTTCTGCTCCCTCCCCTTGCCTTCAGCATCTGGTAAA CACTTTAGAAACTTAAACCACTGTTAGCATGTCAGAATGTAGAAAGGCTGTAGGTTTTTC TTTCTTCTCATCTTGTACCCCAAACAACAACTCTACTGGCAATTGTATTCATGTGACTCC TCTGCTTGGAAACCAGTAAGGATCACCAAGACAAGGGGTCTCACTGCTACCAGACAGATA CCAGCTGTGCAAAAAGTTGCCAGATTCTGTGTATTATTTGGAGAGGGAGAGCTGTAAGAC ACAGGAATCCAATACGTAAGTACTAAAGAGAAAAAGAAACCCCTTTCCCTCATCTATTCC TTATATAGGAAAGAGCCAAAAGACCGTCAAACCTTCTGCTTTGAGCAGAACACACGCCAC AATACAGGCTACCACCAACCGAAGCCACCCTCAGAAAAACCTCTTGGCCTTCCAAAGAAA CTTCCTTAACTGGATCTGCCCTATGGAAAACAAGCTTTTATTTAAAACTGACTTT |
Restriction Sites | NotI-NotI |
ACCN | NM_001693 |
Insert Size | 2940 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001693.2, NP_001684.2 |
RefSeq Size | 3054 bp |
RefSeq ORF | 3054 bp |
Locus ID | 526 |
UniProt ID | P21281 |
Domains | ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N |
Protein Families | Druggable Genome |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Actin Filaments Are Involved in the Coupling of V0-V1 Domains of Vacuolar H+-ATPase at the Golgi Complex *
,null,
The Journal of Biological Chemistry
,PubMed ID 26872971
[ATP6V1B2]
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Other Versions
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RC205447 | ATP6V1B2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2) |
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RC205447L1 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), Myc-DDK-tagged |
CNY 6,208.00 |
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RC205447L2 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), mGFP tagged |
CNY 5,890.00 |
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RC205447L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), Myc-DDK-tagged |
CNY 5,890.00 |
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RC205447L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), mGFP tagged |
CNY 5,890.00 |
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RG205447 | ATP6V1B2 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2) |
CNY 5,408.00 |
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