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APE1 (APEX1) (NM_001641) Human Untagged Clone
CAT＃: SC119121
APEX1 (untagged)-Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APE; APE1; APEN; APEX; APX; HAP1; REF1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001641, the custom clone sequence may differ by one or more nucleotides
ATGCCGAAGCGTGGGAAAAAGGGAGCGGTGGCGGAAGACGGGGATGAGCTCAGGACAGAGCCAGAGGCCA AGAAGAGTAAGACGGCCGCAAAGAAAAATGACAAAGAGGCAGCAGGAGAGGGCCCAGCCCTGTATGAGGA CCCCCCAGATCAGAAAACCTCACCCAGTGGCAAACCTGCCACACTCAAGATCTGCTCTTGGAATGTGGAT GGGCTTCGAGCCTGGATTAAGAAGAAAGGATTAGATTGGGTAAAGGAAGAAGCCCCAGATATACTGTGCC TTCAAGAGACCAAATGTTCAGAGAACAAACTACCAGCTGAACTTCAGGAGCTGCCTGGACTCTCTCATCA ATACTGGTCAGCTCCTTCGGACAAGGAAGGGTACAGTGGCGTGGGCCTGCTTTCCCGCCAGTGCCCACTC AAAGTTTCTTACGGCATAGGCGATGAGGAGCATGATCAGGAAGGCCGGGTGATTGTGGCTGAATTTGACT CGTTTGTGCTGGTAACAGCATATGTACCTAATGCAGGCCGAGGTCTGGTACGACTGGAGTACCGGCAGCG CTGGGATGAAGCCTTTCGCAAGTTCCTGAAGGGCCTGGCTTCCCGAAAGCCCCTTGTGCTGTGTGGAGAC CTCAATGTGGCACATGAAGAAATTGACCTTCGCAACCCCAAGGGGAACAAAAAGAATGCTGGCTTCACGC CACAAGAGCGCCAAGGCTTCGGGGAATTACTGCAGGCTGTGCCACTGGCTGACAGCTTTAGGCACCTCTA CCCCAACACACCCTATGCCTACACCTTTTGGACTTATATGATGAATGCTCGATCCAAGAATGTTGGTTGG CGCCTTGATTACTTTTTGTTGTCCCACTCTCTGTTACCTGCATTGTGTGACAGCAAGATCCGTTCCAAGG CCCTCGGCAGTGATCACTGTCCTATCACCCTATACCTAGCACTGTGA >OriGene 5' read for NM_001641 unedited
TATTTTGTAATACGACTCACTATAGNNGGCGGCCGCGAAATTCGGCACGAGGGTTAGGAG GAGCTAGGCTGCCATCGGGCCGGTGCAGATACGGGGTTGCTCTTTTGCTCATAAGAGGGG CTTCGCTGGCAGTCTGAACGGCAAGCTTGAGTCAGGACCCTTAATTAAGATCCTCAATTG GCTGGAGGGCAGATCTCGCGAGTAGGGCAACGCGGTAAAAATATTGCTTCGGTGGGTGAC GCGGTACAGCTGCCCAAGGGCGTTCGTAACGGGAATGCCGAAGCGTGGGAAAAAGGGAGC GGTGGCGGAAGACGGGGATGAGCTCAGGACAGAGCCAGAGGCCAAGAAGAGTAAGACGGC CGCAAAGAAAAATGACAAAGAGGCAGCAGGAGAGGGCCCAGCCCTGTATGAGGACCCCCC AGATCAGAAAACCTCACCCAGTGGCAAACCTGCCACACTCAAGATCTGCTCTTGGAATGT GGATGGGCTTCGAGCCTGGATTAAGAAGAAAGGATTAGATTGGGTAAAGGAAGAAGCCCC AGATATACTGTGCCTTCAAGAGACCAAATGTTCAGAGAACAAACTACCAGCTGAACTTCA GGAGCTGCCTGGACTCTCTCATCAATACTGGTCAGCTCCTTCGGACAAGGAAGGGTACAG TGGCGTGGGCCTGCTTTCCCGCCAGTGCCCACCTCAAAGTTTCTTACGGCATAAGCGANG AGGAGCATGATCAGGAAGGCCGGGTGATTGTGGCTGAATTTGACTCGTTTGTGCTGTTAA CACCATATGTACCTAATGCACGCCCGAGGTCTGGGTACAACTGAAGTACCCGCCACGCCT GGGATGAAGCCTTTCGCAGATCCCTGAAGGGCCTGGCTTCCCCAAAGCCACTTGTGCTGT GGGNAGACTCCATGTGGACCATTGAAGAAATGACCTCCCCACCCCNCAGGGAACANAAGN AATGCTGCTTCACGCCCCAAGACCGCCACGCTTTTGGGG >OriGene 3' read for NM_001641 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGAAACTATGGCTCTTT ATTTTCATGTGGATAATTCAAACAAAGTCATTAGTAGTCTTTGTTCAATTTTTTTTTAAA AAACAAAAAACCCTCAAATAAAAAATCTTGGGCTTAAAAGAACTCTATCACAGGAGCCTG GTTGGAGGATTCCTAGTTTTATACATGAGAAATAGAATGCAGATTTCTCTGAAGAGTGTT TAAAGAAGGAATGGTAGTTGAGGGGGCTTATTTCCCAGGCTCAAAGTGATTTAGGGGTGG TGTCACAGTGCTAGGTATAGGGTGATAGGACAGTGATCACTGCCGAGGGCCTTGGAACGG ATCTTGCTGTCACACAATGCAGGTAACAGAGAGTGGGACAACAAAAAGTAATCAAGGCGC CAACCAACATTCTTGGATCGAGCATTCATCATATAAGTCCAAAAGGTGTAGGCATAGGGT GTGTTGGGGTAGAAGTGCCTAAAGCTGTCAGCCAGTGGCACAGCCTGCAGTAATTCCCCG AAGCCTTGGCGCTCTTGTGGCGTGAAGCCAGCATTCTTTTTGTTCCCCTTTGGGGTTGCA AAGGTCAATTTCCTTCATGGGGCCACATTTGAGGTCTCCACACAGCACAAGGGGGCTTTT GGGGAAGCCACGGCCCTTCAAGGAACTGGCGAAAAGGCTTTATTCCCAACGCCTTGCCGG TCCTCCAATTCGTACCAAACCCTGGGCCTGCATTAAGGACAATAGGCTGGTTCCCCCCAC AACCGATTCAAATTCAGACCCCATCCCCCGGGCCTCCTCGATAATGGCTCTTCTCCCCCT ATGCGGAAAAAACCTTTGAGGGGGCCCTGGCGGGAAAACAAGCCCCACCCCACTGTTCCC TTTCTGGTCCAGAGGAATTGACCACCTCTGGAGAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001641 |
| Insert Size | 1540 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001641.2, NP_001632.2 |
| RefSeq Size | 1574 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 328 |
| UniProt ID | P27695 |
| Domains | Exo_endo_phos |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Base excision repair |
| Gene Summary | The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.[provided by RefSeq, Dec 2019] Transcript Variant: This variant (1) contains the full-length first exon and is the longest transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Distinct Roles of Ape1 Protein, An Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing
,Wang, H;Wang, X;Chen, G;Zhang, X;Tang, X;Park, D;Cucinotta, FA;Yu, DS;Deng, X;Dynan, WS;Doetsch, PW;Wang, Y;,
J. Biol. Chem.
,PubMed ID 25210033
[APEX1]
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Altered endoribonuclease activity of apurinic/apyrimidinic endonuclease 1 variants identified in the human population
,Kim, WC;Ma, C;Li, WM;Chohan, M;Wilson Iii, DM;Lee, CH;,
PLoS ONE
,PubMed ID 24595156
[APEX1]
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APEX1 Regulation of Aldosterone Synthase Gene Transcription Is Disrupted by a Common Polymorphism in Humans
,Frances McManus, William Sands, Louise Diver, Scott M. MacKenzie, Robert Fraser, Eleanor Davies, and John M. Connell,
Circ. Res., Jul 2012; 111: 212 - 219.
[APEX1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201732 | APEX1 (Myc-DDK-tagged)-Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1 |
CNY 2,400.00 |
|
| RC201732L1 | Lenti ORF clone of Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201732L2 | Lenti ORF clone of Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201732L3 | Lenti ORF clone of Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201732L4 | Lenti ORF clone of Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG201732 | APEX1 (tGFP-tagged) - Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1 |
CNY 4,000.00 |
|
| SC320587 | APEX1 (untagged)-Human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1 |
CNY 2,400.00 |
