EDG2 (LPAR1) (NM_001401) Human Untagged Clone

CAT#: SC119263

LPAR1 (untagged)-Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1



  "NM_001401" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
LPAR1 (EDG2) mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms edg-2; EDG2; Gpcr26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119263 sequence for NM_001401 edited (data generated by NextGen Sequencing)
ATGGCTGCCATCTCTACTTCCATCCCTGTAATTTCACAGCCCCAGTTCACAGCCATGAAT
GAACCACAGTGCTTCTACAACGAGTCCATTGCCTTCTTTTATAACCGAAGTGGAAAGCAT
CTTGCCACAGAATGGAACACAGTCAGCAAGCTGGTGATGGGACTTGGAATCACTGTTTGT
ATCTTCATCATGTTGGCCAACCTATTGGTCATGGTGGCAATCTATGTCAACCGCCGCTTC
CATTTTCCTATTTATTACCTAATGGCTAATCTGGCTGCTGCAGACTTCTTTGCTGGGTTG
GCCTACTTCTATCTCATGTTCAACACAGGACCCAATACTCGGAGACTGACTGTTAGCACA
TGGCTCCTTCGTCAGGGCCTCATTGACACCAGCCTGACGGCATCTGTGGCCAACTTACTG
GCTATTGCAATCGAGAGGCACATTACGGTTTTCCGCATGCAGCTCCACACACGGATGAGC
AACCGGCGGGTAGTGGTGGTCATTGTGGTCATCTGGACTATGGCCATCGTTATGGGTGCT
ATACCCAGTGTGGGCTGGAACTGTATCTGTGATATTGAAAATTGTTCCAACATGGCACCC
CTCTACAGTGACTCTTACTTAGTCTTCTGGGCCATTTTCAACTTGGTGACCTTTGTGGTA
ATGGTGGTTCTCTATGCTCACATCTTTGGCTATGTTCGCCAGAGGACTATGAGAATGTCT
CGGCATAGTTCTGGACCCCGGCGGAATCGGGATACCATGATGAGTCTTCTGAAGACTGTG
GTCATTGTGCTTGGGGCCTTTATCATCTGCTGGACTCCTGGATTGGTTTTGTTACTTCTA
GACGTGTGCTGTCCACAGTGCGACGTGCTGGCCTATGAGAAATTCTTCCTTCTCCTTGCT
GAATTCAACTCTGCCATGAACCCCATCATTTACTCCTACCGCGACAAAGAAATGAGCGCC
ACCTTTAGGCAGATCCTCTGCTGCCAGCGCAGTGAGAACCCCACCGGCCCCACAGAAGGC
TCAGACCGCTCGGCTTCCTCCCTCAACCACACCATCTTGGCTGGAGTTCACAGCAATGAC
CACTCTGTGGTTTAG

Clone variation with respect to NM_001401.3
>OriGene 5' read for NM_001401 unedited
GGGTTCACATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGCTTC
TCCTAGCATGACTTCGATCTGATCAGCAAACAAGAAAATTGTCTCCCGTAGTTCTGGGGC
GTGTTCACCACCTACAACCACAGAGCTGTCATGGCTGCCATCTCTACTTCCATCCCTGTA
ATTTCACAGCCCCAGTTCACAGCCATGAATGAACCACAGTGCTTCTACAACGAGTCCATT
GCCTTCTTTTATAACCGAAGTGGAAAGCATCTTGCCACAGAATGGAACACAGTCAGCAAG
CTGGTGATGGGACTTGGAATCACTGTTTGTATCTTCATCATGTTGGCCAACCTATTGGTC
ATGGTGGCAATCTATGTCAACCGCCGCTTCCATTTTCCTATTTATTACCTAATGGCTAAT
CTGGCTGCTGCAGACTTCTTTGCTGGGTTGGCCTACTTCTATCTCATGTTCAACACAGGA
CCCAATACTCGGAGACTGACTGTTAGCACATGGCTCCTTCGTCAGGGCCTCATTGACACC
AGCCTGACGGCATCTGTGGCCAACTTACTGGCTATTGCAATCGAGAGGACTTTTACGGGT
TTCCGCATGCAGCTCCACACACGGATGAGCAACCGGCGGGTAGTGGTGGTCATTGTGGTC
ATCTGGACTATGGCCATCGTTATGGGTGCTATACCCAGTGTGGGCTGGGACTGTATCTGT
GATATTGAAAATTGTTCCAACATGGCACCCCTCTACAGTGACTCTTACTTAGTCTTCTGG
GCCATTTTCAACTTGGTGACCTTTGTGGAAAGGGGGGGTCTCTATGCTCACACTCTTGGC
TATGGTCGCCAGAGAACATGAGAATGTCTCGGATAATTCTGGACCCCGCCCATCGGGAAA
CATGC
>OriGene 3' read for NM_001401 unedited
CGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGATATGACAATCCTTTATTC
ACTCGGCACATTTGGTTTCTTTGCATTTTCTTCCATTTTACATTGCAGGTGTGGCTACCA
AGAGCTGGATAACGAGTCCCTCAAACAAAGTTTGGAATTGCGAGATATATTGGGGTACCT
TGATTCTTGAGACAGTTGAATTCTTCTAGTTGATCTTGTTGTATTAAAAAGTCACTCTCA
ACTGAAGTGACCACTGCATTTCTTTTGTAAAAAGGTCATTTGACTGGCTTTTCCTCACAA
CTGCCACCCATGCAGTACAAAAGCAGGAAATCCCTTTGCATGAAAGGTGTAAGTACAAGA
TGACATTTCACATTTTTTTAAAAAAAGAATCCTTCATGGGAATATATCCTAATAATCAAT
TATATGGAGACAGTTTTATGTACACCAAATTTCTGCAACTTTATAATAATCAAAATTAGA
AACAACCTAAATAGTTAACAACATGGGAATGGTTAAATAAACTGAGTTGCATCCATTAAA
TGGAATATAATATAGCCATTAAAATTATGTTTTTGTAAAATTTTTAATGCCATAAGAAAA
TGTGGCAATTTTGCAATGAAAAAGATCTACTTATAAAACTGTTTACAGTATGACTCCAAT
TATGTAAAAAAAGTATACAATACACATATAGGCCTACATGGGGGTTGCTTTTTAAGGAGG
CTACTTCTGGGTTGCGATATTATAGAAATATTTTGCCTTTTATACATTNCGTATTTTTCA
AGTTTCTTGATGAGTTATTATTTTCAAAGACTACCAATTTTCCTCTGAATACTGGAATTA
AATGTACCTGGGATTTTTAATTATGGGAACAACTTTAGGCTTCTCTTCTTGACTTTTAGA
TAACTACACTTGGGATAAACTCCTTCCTGTTTAACTGGTC
Restriction Sites NotI-NotI     
ACCN NM_001401
Insert Size 2910 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001401.3, NP_001392.2
RefSeq Size 3104 bp
RefSeq ORF 1095 bp
Locus ID 1902
UniProt ID Q92633
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Gap junction, Neuroactive ligand-receptor interaction
Gene Summary The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Many transcript variants encoding a few different isoforms have been identified for this gene. [provided by RefSeq, Oct 2020]
Transcript Variant: This variant (1) represents the shorter transcript. Variants 1-26 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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