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DAP5 (EIF4G2) (NM_001418) Human Untagged Clone
CAT＃: SC119270
EIF4G2 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1
CNY 6,648.00
CNY 7,220.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAG1; DAP5; NAT1; P97 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119270 sequence for NM_001418 edited (data generated by NextGen Sequencing)
GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGA GGAAGTAGGGGTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGG AAAACCCCAGGGCAAAACGCTCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGAC AACTCCGCAGCAAACAACTCCGCAAACGAAAAAGAACGACATGATGCAATCTTCAGGAAA GTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTGACAAGCTATGCCTTGAGCTC CTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCTGATTGTGGAC AAAGCCCTANNAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGATTGGCA GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGC ACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGAACTAGA AATGTTGATGTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAGA GCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTT GATCTTATTCACGAATCTATCCTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAG AGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGATAATGAGGACA GTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCGA ATGTGCTCCTTGATGTTAAGTAAGGAATTGCCAGCAAGGATTCGTTTCCTGCTGCAGGAT ACCGTAGAGTTGCGAGAACACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCA AAGACGATCAATCAAATTCGTCAAGATGNNNTAAAAGATCTAGGGGTGTTTATTCCTGCT CCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGGAGGGACCGTTCATGCCACCCAGG ATGAAAATGGATAGGGACCCACTTGGAGGACTTGCTGATATGTTTGGACAAATGCCAGGT AGCGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGT CATCGTTCAAATCAACTCTTCAATGGCCATGGGGGACACATCATGCCTCCCACACAATCG CAGTTTGGAGAGATGGGAGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCAT AACCAGAGTCAGGGACTCTTATCCCAGCTGCAAGGACAGTCGAAGGATATGCCACCTCGG TTTTCTAAGAAAGGACAGCTTAATGCAGATGAGATTAGCCTGAGGCCTGCTCAGTCGTTC CTAATGAATAAAAATCAAGTGCCAAAGCTTCAGCCCCAGATAACTATGATTCCTCCTAGT GCACAACCACCACGCACTCAAACACCACCTCTGGGACAGACACCTCAGCTTGGTCTCAAA ACTAATCCACCACTTATCCAGGAAAAGCCTGCCAAGACCAGCAAAAAGCCACCACCGTCA AAGGAAGAACTCCTTAAACTAACTGAAACTGTTGTGACTGAATATCTAAATAGTGGAAAT GCAAATGAGGCTGTCAATGGTGTAAGAGAAATGAGGGCTCCTAAACACTTTCTTCCTGAG ATGTTAAGCAAAGTAATCATCCTGTCACTAGATAGAAGCGATGAAGATAAAGAAAAAGCA AGTTCTTTGATCAGTTTACTCAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAG GCTTTCCTGAATGTATTGGACCAGTGTCCCAAACTGGAGGTTGACATCCCTTTGGTGAAA TCCTATTTAGCACAGTTTGCAGCTCGTGCCATCATTTCAGAGCTGGTGAGCATTTCAGAA CTAGCTCAACCACTAGAAAGTGGCACCCATTTTCCTCTCTTCCTACTTTGTCTTCAGCAG TTAGCTAAATTACAAGATCGAGAATGGTTAACAGAACTTTTTCAACAAAGCAAGGTCAAT ATGCAGAAAATGCTCCCAGAAATTGATCAGAATAAGGACCGCATGTTGGAGATTTTGGAA GGAAAGGGACTGAGTTTCTTATTCCCACTCCTCAAATTGGAGAAGGAACTGTTGAAGCAA ATAAAGTTGGATCCATCCCCTCAAACCATATATAAATGGATTAAAGATAACATCTCTCCC AAACTTCATGTAGATAAAGGATTTGTGAACATCTTAATGACTAGCTTCTTACAGTACATT TCTAGTGAAGTAAACCCCCCCAGCGATGAAACAGATTCATCCTCTGCTCCTTCCAAAGAA CAGTTAGAGCAGGAAAAACAACTACTACTATCTTTCAAGCCAGTAATGCAGAAATTTCTT CATGATCACGTTGATCTACAAGTCAGTGCCCTGTATGCTCTCCAGGTGCACTGCTATAAC AGCAACTTCCCAAAAGGCATGTTACTTCGCTTTTTTGTGCACTTCTATGACATGGAAATT ATTGAAGAAGAAGCTTTCTTGGCTTGGAAAGAAGATATAACCCAAGAGTTTCCGGGAAAA GGCAAGGCTTTGTTCCAGGTGAATCAGTGGCTAACCTGGTTAGAAACTGCTGAAGAAGAA GAATCAGAGGAAGAAGCTGACTAA Clone variation with respect to NM_001418.3 370 g=>n;371 a=>n;989 c=>n;990 a=>n;991 g=>n;1572 g=>a >OriGene 5' read for NM_001418 unedited
AATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCACGCCTTAGCGC CAGCAGTGAGTCGGAGCTCTATGGAGGTGGCAGCGGGTACCGAGTGGCGGCTGCAGCAGC GACTCCTCTGAGCTGAGTTTGAGGCCGTCCCCGACTCCTTCCTCCCCCTTCCCTCCCCCT TTTTTTTGTTTTCCGTTCCCCTTTCCCCTCCCTTCCCTATCCCCGACGACCGGATCCTGA GGAGGCAGCTGCGGTGGCAGCTGCTGAGTTCTCGGTGAAGGTATTTCATTTCTCCTGTCC CCTCCCCTCCCCACCCCATCTATTAATATTATTCTTTTGAAGATTCTTCGTTGTCAAGCC GCCAAAGTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGC GGAGGAGGAAGTAGGGGTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTC CTGGGGAAAACCCCAGGGCAAAACGCTCAGAAATGGATTCCTGCACGAAGCACTAGACGA GATGACAACTCCGCAGCAAACAACTCCGCAAACGAAAAAGAACGACATGATGCAATCTTC AGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTGACAAGCTATGCCTT GAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTANAGGGGTCATACTGCTGATT GTGGACAAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGA TTGGCAGAGATGCACCAAACTTTGATGCCCAGCAGCAGAGGGTCACCAGNACAGAAGCAA GCCCACTTCAGACGCTNCTAATTCC >OriGene 3' read for NM_001418 unedited
NNNAAAAAATTAACTTGAACCGCGCCGCATACTANGATCGATNNTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTCAAAGTTTCACAATGTTTATTGATAAATACAAGTTTAT AAAATCAGGGCATGAACATGACTTGATAAATTAAGTAAACTTAATTTCAATACTATAATA GGAGGGACCAATTCAAATTCTCACCATTTGTTTCACACCCACAAAAACCACTTCAAGGGC ATTAACGATCTCTCAAAACTGATCAGTTTTGTGCAAGTAAACCATGTTTCTTTTAAAAAG ACTTGTGCACTTGCCCAGGCTCAAGTATATTAAATCTACCACATAAAGCCCATTACTAGA GGTAGATTACAGGCAATTACTTTTACGGCAACACCCTCAATTCAGTTAAAATTTTTTCGT AACAACAAATGGATAATCAAATTTTGCAACAACTCAGTTTTACTGACCAAAGGGCTTTTC TACAGTATTCAGGGTTGCTATTAAGTTTTCTAACTAAAAACACCTTATAAAACTGGCAAC AAAAAGGGCCCTTGAATAAACTGCCTTTGCTTGAAACTTATGAAGAAATTTTTGGCGGCT GGTAATTACCTTCTAAACTTTAAAACCCCCTAAAATTTTGGGGGGCCCCTTGGATAAAAC TTCCCCTCAAAAAAACCTTTGCCAAGGAAAACCTACTGGCCGGCCTTGGTTTAAAATTTG GAAAACTTTCCCGGAATGAATTTCCCGGGGGTTGGGAAAGGCCGTTCCTTTAACCCCCGT TTTTTGGCAAATTAAAGGTTGGCGGGGTTTTAAAAAACTTTTCTCTTATTTTTTTTTTAA AAGTTTTACACGGTTTCCCCCTTTTTCCCTGAAAAAAACTTGTCTTTTCCGAGAATTTTG GGTAATTTTTTTTCAAACCAAAAGCTTTTTTAAAATTCCCGGCAAAAAGGCCAAAAAAAA AATCACTCCTTTTGAAATTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001418 |
| Insert Size | 3860 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001418.1, NP_001409.1 |
| RefSeq Size | 3820 bp |
| RefSeq ORF | 2724 bp |
| Locus ID | 1982 |
| UniProt ID | P78344 |
| Domains | eIF5C, MA3 |
| Protein Families | Transcription Factors |
| Protein Pathways | Viral myocarditis |
| Gene Summary | Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (1). Both variants 1 and 3 encode the same isoform. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207227 | EIF4G2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1 |
CNY 6,640.00 |
|
| RC207227L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207227L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207227L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207227L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1, mGFP tagged |
CNY 9,040.00 |
|
| RG207227 | EIF4G2 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 1 |
CNY 8,240.00 |
