Hsp47 (SERPINH1) (NM_001235) Human Untagged Clone
CAT#: SC119367
SERPINH1 (untagged)-Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2
CNY 3,656.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AsTP3; CBP1; CBP2; gp46; HSP47; OI10; PIG14; PPROM; RA-A47; SERPINH2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC119367 sequence for NM_001235 edited (data generated by NextGen Sequencing)
ATGCGCTCCCTCCTGCTTCTCAGCGCCTTCTGCCTCCTGGAGGCGGCCCTGGCCGCCGAG GTGAAGAAACCTGCAGCCGCAGCAGCTCCTGGCACTGCGGAGAAGTTGAGCCCCAAGGCG GCCACGCTTGCCGAGCGCAGCGCCGGCCTGGCCTTCAGCTTGTACCAGGCCATGGCCAAG GACCAGGCAGTGGAGAACATCCTGGTGTCACCCGTGGTGGTGGCCTCGTCGCTGGGGCTC GTGTCGCTGGGCGGCAAGGCGACCACGGCGTCGCAGGCCAAGGCAGTGCTGAGCGCCGAG CAGCTGCGCGACGAGGAGGTGCACGCCGGCCTGGGCGAGCTGCTGCGCTCACTCAGCAAC TCCACGGCGCGCAACGTGACCTGGAAGCTGGGCAGCCGACTGTACGGACCCAGCTCAGTG AGCTTCGCTGATGACTTCGTGCGCAGCAGCAAGCAGCACTACAACTGCGAGCACTCCAAG ATCAACTTCCGCGACAAGCGCAGCGCGCTGCAGTCCATCAACGAGTGGGCCGCGCAGACC ACCGACGGCAAGCTGCCCGAGGTCACCAAGGACGTGGAGCGCACGGACGGCGCCCTGTTA GTCAACGCCATGTTCTTCAAGCCACACTGGGATGAGAAATTCCACCACAAGATGGTGGAC AACCGTGGCTTCATGGTGACTCGGTCCTATACCGTGGGTGTCATGATGATGCACCGGACA GGCCTCTACAACTACTACGACGACGAGAAGGAAAAGCTGCAAATCGTGGAGATGCCCCTG GCCCACAAGCTCTCCAGCCTCATCATCCTCATGCCCCATCACGTGGAGCCTCTCGAGCGC CTTGAAAAGCTGCTAACCAAAGAGCAGCTGAAGATCTGGATGGGGAAGATGCAGAAGAAG GCTGTTGCCATCTCCTTGCCCAAGGGTGTGGTGGAGGTGACCCATGACCTGCAGAAACAC CTGGCTGGGCTGGGCCTGACTGAGGCCATTGACAAGAACAAGGCCGACTTGTCACGCATG TCAGGCAAGAAGGACCTGTACCTGGCCAGCGTGTTCCACGCCACCGCCTTTGAGTTGGAC ACAGATGGCAACCCCTTTGACCAGGACATCTACGGGCGCGAGGAGCTGCGCAGCCCCAAG CTGTTCTACGCCGACCACCCCTTCATCTTCCTAGTGCGGGACACCCAAAGCGGCTCCCTG CTATTCATTGGGCGCCTGGTCCGGCCTAAGGGTGACAAGATGCGAGACGAGTTATAG Clone variation with respect to NM_001235.3 234 a=>g;598 c=>t >OriGene 5' read for NM_001235 unedited
TTTGGATTTGTAATACGACTTTCCTATAGGCGGCCGACGAATTCGCACGAGGCGCGGCTC GAGAGCGAGAGTCACGTCCCGGCGCTAGCCCAGCCCGACCCATGCCCACCGTGGTGCACG CAAACCACTTCCTGGCCATGCGCTCCCTCCTGCTTCTCAGCGCCTTCTGCCTCCTGGAGG CGGCCCTGGCCGCCGAGGTGAAGAAACCTGCAGCCGCAGCAGCTCCTGGCACTGCGGAGA AGTTGAGCCCCAAGGCGGCCACGCTTGCCGAGCGCAGCGCCGGCCTGGCCTTCAGCTTGT ACCAGGCCATGGCCAAGGACCAGGCAGTGGAGAACATCCTGGTGTCACCCGTGGTGGTGG CCTCGTCGCTGGGGCTCGTGTCGCTGGGCGGCAAGGCGACCACGGCGTCGCAGGCCAAGG CAGTGCTGAGCGCCGAGCAGCTGCGCGACGAGGAGGTGCACGCCGGCCTGGGCGAGCTGC TGCGCTCACTCAGCAACTCCACGGCGCGCAACGTGACCTGGAAGCTGGGCAGCCGACTGT ACGGACCCAGCTCAGTGAGCTTCGCTGATGACTTCGTGCGCAGCAGCAAGCAGCACTACA ACTGCGAGCACTCCAAGATCAACTTCCGCGACAAGCGCAGCGCGCTGCAGTCCATCAACG AGTGGGCCGCGCATACCACCGACAGCNAGCTGCCCGATGTCACCAAGGACGTGGAGCGCA CGGACGGCGCCCTGTTAGTCAACGCCATGTTCTTCAGCCACACTGGGATGAGAAATTCAC CCACAGATGGTGGGACACCGTGGCTTTCTGGTGACTCGGTCCTTACCGTGGGTGTCATGA TGATGCACCGGACAGCCTCTACACTACTACGACGACGAGAAGAATAGCTGCAAATCGTGG AGATGCCCCTGGCCACAAGCTCTCN >OriGene 3' read for NM_001235 unedited
TTATGGAACGCGGCCCGCTTTTCTAGAAGTCGAAGTTTTTTCCTTTTTTTTTTTGGCATT GGGAAAGTTTTATTGAAAAAAAAAAGTACAAATAAGTTCTTGGATTGATAGCAACAAAGG CTCATGTTCCCCCTTCCCTCCCTATCTTTGAAGAACTAAAAAAGGAAGAAACAAAACAAA AAGTTCATCCCCACAACGCCAGACACGATGCTTCTTGACCAGAGTCTGCCCAGAAGCCCC TCCTGGGAGCTCCTTCTCAATCCGCCTCACTGCGGCCAGGTCATTCTGGGGGTGCCTGGT CCCAGGGGCTGCAGCACCTAGTTTTATAGTTGGGAGAGGTTGGGATAGAGCTGGGGAGGC AGCTGAGGTGTTTTAGTGTCAGAAGAGGGCTGGCTGACCCCCCTAGCTCAATTTGGTCTC ACAGGTGAGAAGGTACCTGGCTATAAATATGAATACTGATTGAGGCAGGCTTGATCTGGG ACTTTCAGGGCAGGCAGAATGACTATGGCCCAGGTCCCACAGGATGTGGAGTTTCCGGGC TCAGCATCATGGTATCTGGGGCCCATGTCCAAGTGGAGTGAATTCTAGCTGGGAAGGTCC GCTCAAGCACACGGGAACCCCGGTCTGCTTTTCCACCCCCACCCCATGGAGTATCCAAGG CTGGTACCTCACCTTCCCCCCAACCCCATAGCACCCATGTGTCTCAGGAGCCTTTGGATG CCTCCTGCCATCCTGTGTGCACCCCTGAGCCCCTATACTCGGCTTGCATCTTGTCACCCC TTAAGCCGGACCAGCCGCCCAATGGATAACAGGAAGCCGCTTTTGTTCGTCCCGACCTAC GAAGAAGAAAGGCGGGGGCCGCGTATAACAGCTTGGAGGCTGGCCAACTCCTCCGCCCGC AAAGGCCCTGCCAAAGGGTTGCCATCTGTGGCCACTCAAGGCGGGGGGTGGAACAGCTGT CCAGTACAGGGCCTTTTGCTGAATGGGGAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_001235 |
Insert Size | 2250 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001235.2, NP_001226.2 |
RefSeq Size | 2208 bp |
RefSeq ORF | 1257 bp |
Locus ID | 871 |
UniProt ID | P50454 |
Domains | SERPIN |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Over-Expression of HSP47 Augments Mouse Embryonic Stem Cell Smooth Muscle Differentiation and Chemotaxis
,Wong, MM;Yin, X;Potter, C;Yu, B;Cai, H;Di Bernardini, E;Xu, Q;,
PLoS ONE, Jan 2014.
,PubMed ID 24454956
[SERPINH1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209847 | SERPINH1 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2 |
CNY 3,656.00 |
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RC209847L1 | Lenti ORF clone of Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC209847L2 | Lenti ORF clone of Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC209847L3 | Lenti ORF clone of Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC209847L4 | Lenti ORF clone of Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2, mGFP tagged |
CNY 6,056.00 |
|
RG209847 | SERPINH1 (tGFP-tagged) - Human serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant 2 |
CNY 5,256.00 |