Amino terminal enhancer of split (AES) (NM_001130) Human Untagged Clone

CAT#: SC119415

AES (untagged)-Human amino-terminal enhancer of split (AES), transcript variant 2



  "NM_001130" in other vectors (4)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 5 publications.

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-AES Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AES; AES-1; AES-2; ESP1; GRG; Grg-5; GRG5
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119415 sequence for NM_001130 edited (data generated by NextGen Sequencing)
ATGATGTTTCCACAAAGCAGGCATTCGGGCTCCTCGCACCTACCCCAGCAACTCAAATTC
ACCACCTCGGACTCCTGCGACCGCATCAAAGACGAATTTCAGCTACTGCAAGCTCAGTAC
CACAGCCTCAAGCTCGAATGTGACAAGTTGGCCAGTGAGAAGTCAGAGATGCAGCGTCAC
TATGTGATGTACTACGAGATGTCCTACGGCTTGAACATCGAGATGCACAAACAGGCTGAG
ATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCTGCCCTACCTCTCCCAAGAGCAC
CAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCAGGTCACCGCTCCCGAGCTGAAC
TCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTCCCAGCTGCAGGCCCTGGCCCTG
CCCTTGACCCCACTACCCGTGGGGCTGCAGCCGCCTTCGCTGCCGGCGGTCAGCGCAGGC
ACCGGCCTCCTCTCGCTGTCCGCGCTGGGTTCCCAGGCCCACCTCTCCAAGGAAGACAAG
AACGGGCACGATGGTGACACCCACCAGGAGGATGATGGCGAGAAGTCGGATTAG

Clone variation with respect to NM_001130.5
>OriGene 5' read for NM_001130 unedited
TAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGATCGGCGCCGGGACAA
AGGCGCGGCCGCCCGGCGCCCCCAGCAGCCCGAGCCGGGGCGCACAGCCGGGGCGCAGCC
CGCGCCCCCCGCCGCGATTGACATGATGTTTCCACAAAGCAGGCATTCGGGCTCCTCGCA
CCTACCCCAGCAACTCAAATTCACCACCTCGGACTCCTGCGACCGCATCAAAGACGAATT
TCAGCTACTGCAAGCTCAGTACCACAGCCTCAAGCTCGAATGTGACAAGTTGGCCAGTGA
GAAGTCAGAGATGCAGCGTCACTATGTGATGTACTACGAGATGTCCTACGGCTTGAACAT
CGAGATGCACAAACAGGCTGAGATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCT
GCCCTACCTCTCCCAAGAGCACCAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCA
GGTCACCGCTCCCGAGCTGAACTCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTC
CCAGCTGCAGGCCCTGGCCCTGCCCTTGACCCCACTACCCGTGGGGCTGCAGCCGCCTTC
GCTGCCGGCGGTCAGCGCAGGCACCGGCCTNCTCTCGCTGTCCGCGCTGGGTTCCCAGGC
CCACCTCTCCAGGGAGACAGAACGGGCACGATGGTGACACCCACCAGGAGGATGATGGCG
AGAAAGTCGATTANCAAGGNGGCCGGACGGNGAGGGTTGGAGGGNGGACCAGAGGGANAC
AGAGGCACNGAGAGAAAAGGATGTTTATCACAAGACACAGCGGAGCTCGGGGATGGNCTA
ACTCCATAGTATTTATGGTGGCCGNNCGCGNNGGCCCCAGCCAGCTTGCAAGCCCACTNT
AGCTTTNTTNNCTACCCCATACGGNNNTTCTTCCTCCTCCCNTGCAGNCTGGNTATGTGG
ATACCTGCCCTGACTGGTTGAGCAAGCTAGG
>OriGene 3' read for NM_001130 unedited
CGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACT
TCTCACATCTTTATTGGAAAGGCACAGCTAAGCCCACCCTTGATACAGCATTTTCCACTT
CTCTCTGTAGAGATCAGACGATTGAACACGAGATAAGCTGACTATATACAGACACAGGTG
TGGGTGTTGCTTTTTTTTAAAAACACTTTTGGCTTTTTTTTTTTTTAATATCCCCTTTCT
TAAAAGACAAGCTAGTATACTGGAAAAAGAAAAAAATAATAATAAAATAAAAACCAAGAC
AACTTTAGTACCCTCATCTTTATTTGGGAAGGGGAGGGGGAATCCTGGGTCGCCCACCCT
TACCCTGGTCCTCCCAGCTCAGCTAAGCTCGTCCCTCGTGCCCCCCCTTTTGTGGGCGAT
GGGAAAGGACCAGGTGGGCGTGGAGGTGTCTGGAACTAACAAAGGTGGTGAGTGGGGCAA
GTGGAGGTGGGAGCATACCTGGGACCCGGGGTCGGGGGAGACTCGGGGTGCCCAGGACGG
GAAAGGGGCAGCTAGCATTGCGTGCATGCAGTACCAGGGTGAGAGGGCTGTGGCCCAGGC
AGACTGTCGGTTACACATGTTCAAAACGGGGGAAGGGCCGGGGCTGCTGCGCTTCGCGAG
GTCTTGCTCCCTTGGGACCTGGTCTCCCATCTGACCCTCCAGGCCTTAGCTTGCCTCACA
CGTCAGGGCAGGTATCCACCTAACGGNCTGCAGGGGAGGAGGAGGGAANCCCGGATGGGG
TANGAAAAAGCTANAGTGGCCTGCAACTGGCTGGGGCCCCGCCGCGGCCCATAATACTTG
GGATTATCCATCCGACTCGCTGGTCTGGCTAAATTCTCTTCGCGCCCGGGTCCCCCTTGC
CCCCTTCAACTCCGGTCGGCCCTGTATACGATTCGCTATCCCTGGGG
Restriction Sites NotI-NotI     
ACCN NM_001130
Insert Size 1650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001130.5, NP_001121.2
RefSeq Size 1687 bp
RefSeq ORF 594 bp
Locus ID 166
UniProt ID Q08117
Domains TLE_N
Protein Families Druggable Genome, Transcription Factors
Gene Summary The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (5)

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