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PLOD3 (NM_001084) Human Untagged Clone
CAT＃: SC119470
PLOD3 (untagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3)
CNY 8,120.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LH3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119470 sequence for NM_001084 edited (data generated by NextGen Sequencing)
ATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCCGCTGCTGCTGCCCCCT GCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCCAGAGAAGCTGCTG GTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCTGCGCTCTGCGGAG TTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCGAGGGGGTGATGTG GCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGAAATGGAGAAATAC GCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGTGATTCTGGCCGGC AGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCTGCTCTTCTCTGCA GAGAGCTTCTGCTGGCCCGAGTGGGGGCTGGCGGAGCAGTACCCTGAGGTGGGCACGGGG AAGCGCTTCCTCAATTCTGGTGGATTCATCGGTTTTGCCACCACCATCCACCAAATCGTG CGCCAGTGGAAGTACAAGGATGATGACGACGACCAGCTGTTCTACACACGGCTCTACCTG GACCCAGGACTGAGGGAGAAACTCAGCCTTAATCTGGATCATAAGTCTCGGATCTTTCAG AACCTCAACGGGGCTTTAGATGAAGTGGTTTTAAAGTTTGATCGGAACCGTGTGCGTATC CGGAACGTGGCCTACGACACGCTCCCCATTGTGGTCCATGGAAACGGTCCCACTAAGCTG CAGCTCAACTACCTGGGAAACTACGTCCCCAATGGCTGGACTCCTGAGGGAGGCTGTGGC TTCTGCAACCAGGACCGGAGGACACTCCCGGGGGGGCAGCCTCCCCCCCGGGTGTTTCTG GCCGTGTTTGTGGAACAGCCTACTCCGTTTCTGCCCCGCTTCCTGCAGCGGCTGCTACTC CTGGACTATCCCCCCGACAGGGTCACCCTTTTCCTGCACAACAACGAGGTCTTCCATGAA CCCCACATCGCTGACTCCTGGCCGCAGCTCCAGGACCACTTCTCAGCTGTGAAGCTCGTG GGGCCGGAGGAGGCTCTGAGCCCAGGCGAGGCCAGGGACATGGCCATGGACCTGTGTCGG CAGGACCCCGAGTGTGAGTTCTACTTCAGCCTGGACGCCGACGCTGTCCTCACCAACCTG CAGACCCTGCGTATCCTCATTGAGGAGAACAGGAAGGTGATCGCCCCCATGCTGTCCCGC CACGGCAAGCTGTGGTCCAACTTCTGGGGCGCCCTGAGCCCCGATGAGTACTACGCCCGC TCCGAGGACTACGTGGAGCTGGTGCAGCGGAAGCGAGTGGGTGTGTGGAATGTACCATAC ATCTCCCAGGCCTATGTGATCCGGGGTGATACCCTGCGGATGGAGCTGCCCCAGAGGGAT GTGTTCTCGGGCAGTGACACAGACCCGGACATGGCCTTCTGTAAGAGCTTTCGAGACAAG GGCATCTTCCTCCATCTGAGCAATCAGCATGAATTTGGCCGGCTCCTGGCCACTTCCAGA TACGACACGGAGCACCTGCACCCCGACCTCTGGCAGATCTTCGACAACCCCGTCGACTGG AAGGAGCAGTACATCCACGAGAACTACAGCCGGGCCCTGGAAGGGGAAGGAATCGTGGAG CAGCCATGCCCGGACGTGTACTGGTTCCCACTGCTGTCAGAACAAATGTGTGATGAGCTG GTGGCAGAGATGGAGCACTACGGCCAGTGGTCAGGCGGCCGGCATGAGGATTCAAGGCTG GCTGGAGGCTACGAGAATGTGCCCACCGTGGACATCCACATGAAGCAGGTGGGGTACGAG GACCAGTGGCTGCAGCTGCTGCGGACGTATGTGGGCCCCATGACCGAGAGCCTGTTTCCC GGTTACCACACCAAGGCGCGGGCGGTGATGAACTTTGTGGTTCGCTACCGGCCAGACGAG CAGCCGTCTCTGCGGCCACACCACGACTCATCCACCTTCACCCTCAACGTTGCCCTCAAC CACAAGGGCCTGGACTATGAGGGAGGTGGCTGCCGCTTCCTGCGCTACGACTGTGTGATC TCCTCCCCGAGGAAGGGCTGGGCACTCCTGCACCCCGGCCGCCTCACCCACTACCACGAG GGGCTGCCAACGACCTGGGGCACACGCTACATCATGGTGTCCTTTGTCGACCCCTGA Clone variation with respect to NM_001084.4 >OriGene 5' read for NM_001084 unedited
GGTGGATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCTGTGGCGC CGGCATCTGGAGCTTTCTGTAGCCTCCGGATACGCCTTTTTTTCAGGGCGTAGCCCCAGC CAAGCTGCTCCCCGCGGCGGCCGCACAGCAGCCCGAGCGCCCCCTTTCCAGAGCTCCCCT CCGGAGCTGGGATCCAGGCGCGTAGCGGAGATCCCAGGATCCTGGGTGCTGTCTGGGCCC GCTCCCCACCATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCCGCTGCT GCTGCCCCCTGCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCCAGA GAAGCTGCTGGTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCTGCG CTCTGCGGAGTTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCGAGG GGGTGATGTGGCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGAAAT GGAGAAATACGCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGTGAT TCTGGCCGGCAGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCTGCT CTTCTCTGCAGAGAGCTTCTGCTGGCCCGAGTGGGGGCTGGCGGAGCAGTACCCTGAGGT GGGCACGGGGAAAGCGCTTCCTCCATTCTTGTGGATTCATCCGGTTTTGCCACACCATCC ACCATATCGTGCGCCAGTGGCAGTACAANGATGATGACGACGACCCGCTGTTCTACACAC GCTCTACCTGGAACCAGGACTGAAGGAGGAACTCACCCTTATCCTGGATCTAAGTCTTCG GATCTTTCAGAAC >OriGene 3' read for NM_001084 unedited
GCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACTTACAAAC TGTCCTTTATTCAAAGGGAGACTGCGGAACATTAATAATTTATCACGCGGGGAGTCCCCA AAAGCCCTGTGCCCACGAACCCCTGTGGGCGGAGGAAAAAGGCGGGGACTCCGGGAGCTT CCTGAGAGGGCCGTGTCTTGGGAGCAAGGTGACATATTCAGTTCAGGCACGCGGAACATG AACTCAGGAAGGGGGGAGACAGAGAGACCCATCCCCCAACTCCCAGGACGGGGGCCAGGC CCCCTAAAAAGGCACATTGGCAGGGCAGGTTTGGCAAAGGGGTTGAGTGTCAGGGGTCGA CAAAGGACACCATGATGTAGCGTGTGCCCCAGGTCGTTGGCAGCCCCTCGTGGTATTGGG TGAGGCGGCCGGGGTGCAGGATTGCCCACCCCTTCCTCGGGGAGGAGATCACACAGTCGT AGCGCAGGAAGCGGCAGCCACCTCCCTCATAGTCCAGGCCCTTGTGGTTGAGGGCAACGT TGAGGGTGAAGGTGGATGATTCGTGGTGTGGCCGCATAGACGGCTGCTCGTCTGGCCGGT AGCGAACCACAAAGTTCATCACCGCCCGCGCCTTGTTGTGGAACCCGGGAAACGGCCTCT CGGTCATGGGGCCCACATACGTCCGCAGCAGCTGCAGCCCCTGGTCCTCGTACCCACCTG CTTCATGTGAATGTCACCGTGGGCACTTTCTCGTAGCCTCAGCCAGCCTTGATTCTAATG CCGGCGCCTGACCCTGGCCGTAGGCTCATTTTTGCACCAGCTTATAAAATTTGTTTTGAC GGAGTGGGACCATTCCCGTCCGGCATGTTTGTCCAGTATTCTTCCCTCCAGGGCCAGCTG TATTCTCGGGGAGACCGCTCCTCACCCAGGGNTTGCAAATACTGCAAGGCGGGGGAGTGC TCCGGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001084 |
| Insert Size | 2710 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001084.4, NP_001075.1 |
| RefSeq Size | 2995 bp |
| RefSeq ORF | 2217 bp |
| Locus ID | 8985 |
| UniProt ID | O60568 |
| Domains | 2OG-FeII_Oxy, P4Hc |
| Protein Pathways | Lysine degradation |
| Gene Summary | The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Collagen beta (1- O ) galactosyltransferase 1(GLT25D1)is required for the secretion of high molecular weight adiponectin and affects lipid accumulation
,Webster, JA;Yang, Z;Kim, YH;Loo, D;Mosa, RM;Li, H;Chen, C;,
Biosci. Rep.
,PubMed ID 28428430
[PLOD3]
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Other Versions
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| RG203445 | PLOD3 (tGFP-tagged) - Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3) |
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| SC324563 | PLOD3 (untagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3) |
CNY 8,120.00 |
