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RPB2 (POLR2B) (NM_000938) Human Untagged Clone
CAT＃: SC119548
POLR2B (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B)
CNY 8,184.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hRPB140; POL2RB; RPB2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000938 |
| Insert Size | 4150 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000938.1, NP_000929.1 |
| RefSeq Size | 3748 bp |
| RefSeq ORF | 3525 bp |
| Locus ID | 5431 |
| UniProt ID | P30876 |
| Domains | RNA_pol_Rpb2_6, RNA_pol_Rpb2_7, RNA_pol_Rpb2_2, RNA_pol_Rpb2_1, RNA_pol_Rpb2_3, RNA_pol_Rpb2_4, RNA_pol_Rpb2_5 |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| Gene Summary | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200440 | POLR2B (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 8,176.00 |
|
| RC200440L3 | Lenti-ORF clone of POLR2B (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 13,590.00 |
|
| RC200440L4 | Lenti-ORF clone of POLR2B (mGFP-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 13,590.00 |
|
| RG200440 | POLR2B (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B) |
CNY 12,830.00 |
