CYP51A1 (NM_000786) Human Untagged Clone
CAT#: SC119686
CYP51A1 (untagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1
CNY 3,800.00
Product images
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC119686 sequence for NM_000786 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCGGCTGGGATGCTGCTGCTGGGCTTGCTGCAGGCGGGTGGGTCGGTGCTG GGCCAGGCGATGGAGAAGGTGACAGGCGGCAACCTCTTGTCCATGCTGCTGATCGCCTGC GCCTTCACCCTCAGCCTGGTCTACCTGATCCGTCTGGCCGCCGGCCACCTGGTCCAGCTG CCCGCAGGGGTGAAAAGTCCTCCATACATTTTCTCCCCAATTCCATTCCTTGGGCATGCC ATAGCATTTGGGAAAAGTCCAATTGAATTTCTAGAAAATGCATATGAGAAGTATGGACCT GTATTTAGTTTTACCATGGTAGGCAAGACATTTACTTACCTTCTGGGGAGTGATGCTGCT GCACTGCTTTTTAATAGTAAAAATGAAGACCTGAATGCAGAAGATGTCTACAGTCGCCTG ACAACACCTGTGTTTGGGAAGGGAGTTGCATACGATGTGCCTAATCCAGTTTTCTTGGAG CAGAAGAAAATGTTAAAAAGTGGCCTTAACATAGCCCACTTTAAACAGCATGTTTCTATA ATTGAAAAAGAAACAAAGGAATACTTTGAGAGTTGGGGAGAAAGTGGAGAAAAAAATGTG TTTGAAGCTCTTTCTGAGCTCATAATTTTAACAGCTAGCCATTGTTTGCATGGAAAGGAA ATCAGAAGTCAACTCAATGAAAAGGTAGCACAGCTGTATGCAGATTTGGATGGAGGTTTC AGCCATGCAGCCTGGCTCTTACCAGGTTGGCTGCCTTTGCCTAGTTTCAGACGCAGGGAC AGAGCTCATCGGGAAATCAAGGATATTTTCTATAAGGCAATCCAGAAACGCAGACAGTCT CAAGAAAAAATTGATGACATTCTCCAAACTTTACTAGATGCTACATACAAGGATGGGCGT CCTTTGACTGATGATGAAGTAGCAGGGATGCTTATTGGATTACTCTTGGCAGGGCAGCAT ACATCCTCAACTACTAGTGCTTGGATGGGCTTCTTTTTGGCCAGAGACAAAACACTTCAA AAAAAATGTTATTTAGAACAGAAAACAGTCTGTGGAGAGAATCTGCCTCCTTTAACTTAT GACCAGCTCAAGGATCTAAATTTACTTGATCGCTGTATAAAAGAAACATTAAGACTTAGA CCTCCTATAATGATCATGATGAGAATGGCCAGAACTCCTCAGACTGTGGCAGGGTATACC ATTCCTCCAGGACATCAGGTGTGTGTTTCTCCCACTGTCAATCAAAGACTTAAAGACTCA TGGGTAGAACGCCTGGACTTTAATCCTGATCGCTACTTACAGGATAACCCAGCATCAGGG GAAAAGTTTGCCTATGTGCCATTTGGAGCTGGGCGTCACCGTTGTATTGGGGAAAATTTT GCCTATGTTCAAATTAAGACAATTTGGTCCACTATGCTTCGTTTATATGAATTTGATCTC ATTGATGGATACTTTCCCACTGTGAATTATACAACTATGATTCACACCCCTGAAAACCCA GTTATCCGTTACAAACGAAGATCAAAATGA Clone variation with respect to NM_000786.3 1359 t=>c >OriGene 5' read for NM_000786 unedited
GGATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCTGCCAGCTT CTCTCGTTCCGTCGATTGGGAGGAGCGGTGGCGACCTCGGCCTTCAGTGTTTCCGACGGA GTGAATGGCGGCGGCGGCTGGGATGCTGCTGCTGGGCTTGCTGCAGGCGGGTGGGTCGGT GCTGGGCCAGGCGATGGAGAAGGTGACAGGCGGCAACCTCTTGTCCATGCTGCTGATCGC CTGCGCCTTCACCCTCAGCCTGGTCTACCTGATCCGTCTGGCCGCCGGCCACCTGGTCCA GCTGCCCGCAGGGGTGAAAAGTCCTCCATACATTTTCTCCCCAATTCCATTCCTTGGGCA TGCCATAGCATTTGGGAAAAGTCCAATTGAATTTCTAGAAAATGCATATGAGAAGTATGG ACCTGTATTTAGTTTTACCATGGTAGGCAAGACATTTACTTACCTTCTGGGGAGTGATGC TGCTGCACTGCTTTTTAATAGTAAAAATGAAGACCTGAATGCAGAAGATGTCTACAGTCG CCTGACAACACCTGTGTTTGGGAAAGGGAGTTGCATACGATGTGCCTAATCCAGTTTTCT TGGAGCAGAAGAAATGTTAAAAAGTGGCCTTACATAGCCCACTTTAACAGCATGTTTCTA TTATTGAAAAAGAACAAGGAATCTTTGAGAGTTGGGGAAGAAAGTGAGAAAAAATGGGGT TGAAGCTCTTCTGACTCATATTTTACAGCTAGCCATTGTTGCATGGAAAGGAATTCAAAG TCACTCATGGAAAGGTACACAGCGATGCAATTGNNNGGGGGGGGGGTTTAACCTTGCACC TGGCTTTACCCGGTTGCTGGCTGT >OriGene 3' read for NM_000786 unedited
GTACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTCAAAAAAAATTTTATTCTGAAAATTACCAAAAATTTTGCCTTCCCA TTTAAAGTTTAACATGAGTCTTCCAGGGTTTAAAAAAAACCTGTATTTTCCTTAAGGCAA ATTTTTTGGGCTTCCCCTAGGGAAACCAACCGATGGGGTTTTTTTTCACCCTTCTCATTT AGTTGGGCCCCGAGTTCCAATTGGAAATAAGTGGATTCCTAAACAAAAGGGGCTTTACAA AAGGGGCCCATTTTTTTTCCTCCCAGAGGCCCTTGTTTCAAATTTTTTTAAACCCAAAGT TCACTGATCTCACTTTTAAAAAAAAAAAAAGTTGGTTACCGGTTTTAATCAATTTGAGTG GTACCAACCTTCCCTAATGGGATTCTAAAAAGGAACCAGGGAAGGCCAGGGGCGGGGGCT CATGCCTGTAATCCCACCCCTTTGGGAGGCCAAGGGGGGGGGATCACAAGGTCAGGATTT CAAAACCACCCTACCCAACTTAGGGAACCCCCTGTCTTTCCTAAAAATCCAAAAATTTAC CCAGCCTTGGGGGCAGGGCCCTGTAATCCCAACTCCTTGGGAGGCTGAGGCAAAAAATTG CTTGACCCCGGGAGGCAAAGGATGCATTGACCTGAAATCGCCCCACTGCCTCTCAGCCTG GGCAACAGAGCGAGACTCCTTCTCAAAAAAAAAAAAAAAAGGGAAGCGGGGAACACCTGA GCCAAAGCAAATTATCCTCTAGGACTTGACTTTGGACTCCACATAAGGGGACCTTGGAAA ACTAAATCCTTTTTTCAGGAAAAAAATTGGTATTTTTTANAATTTGACCAAGAACTGGAC CGCCTTTACATAATAATTACATTTTTTAGGATTCTATCAATTGGCN |
Restriction Sites | NotI-NotI |
ACCN | NM_000786 |
Insert Size | 3090 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000786.2, NP_000777.1 |
RefSeq Size | 3381 bp |
RefSeq ORF | 1530 bp |
Locus ID | 1595 |
UniProt ID | Q16850 |
Domains | p450 |
Protein Families | Druggable Genome, P450, Transmembrane |
Protein Pathways | Metabolic pathways, Steroid biosynthesis |
Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
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RC207078 | CYP51A1 (Myc-DDK-tagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1 |
CNY 3,792.00 |
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RC207078L3 | Lenti ORF clone of Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
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RC207078L4 | Lenti ORF clone of Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
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RG207078 | CYP51A1 (tGFP-tagged) - Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1 |
CNY 5,392.00 |
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SC322571 | CYP51A1 (untagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1 |
CNY 3,800.00 |
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