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ADH1B (NM_000668) Human Untagged Clone
CAT＃: SC119751
ADH1B (untagged)-Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B)
CNY 5,488.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADH2; HEL-S-117 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119751 sequence for NM_000668 edited (data generated by NextGen Sequencing)
ATGAGCACAGCAGGAAAAGTAATCAAATGCAAAGCAGCTGTGCTATGGGAGGTAAAGAAA CCCTTTTCCATTGAGGATGTGGAGGTTGCACCTCCTAAGGCTTATGAAGTTCGCATTAAG ATGGTGGCTGTAGGAATCTGTCGCACAGATGACCACGTGGTTAGTGGCAACCTGGTGACC CCCCTTCCTGTGATTTTAGGCCATGAGGCAGCCGGCATCGTGGAGAGTGTTGGAGAAGGG GTGACTACAGTCAAACCAGGTGATAAAGTCATCCCGCTCTTTACTCCTCAGTGTGGAAAA TGCAGAGTTTGTAAAAACCCGGAGAGCAACTACTGCTTGAAAAATGATCTAGGCAATCCT CGGGGGACCCTGCAGGATGGCACCAGGAGGTTCACCTGCAGGGGGAAGCCCATTCACCAC TTCCTTGGCACCAGCACCTTCTCCCAGTACACGGTGGTGGATGAGAATGCAGTGGCCAAA ATTGATGCAGCCTCGCCCCTGGAGAAAGTCTGCCTCATTGGCTGTGGATTCTCGACTGGT TATGGGTCTGCAGTTAACGTTGCCAAGGTCACCCCAGGCTCTACCTGTGCTGTGTTTGGC CTGGGAGGGGTCGGCCTATCTGCTGTTATGGGCTGTAAAGCAGCTGGAGCAGCCAGAATC ATTGCGGTGGACATCAACAAGGACAAATTTGCAAAGGCCAAAGAGTTGGGTGCCACTGAA TGCATCAACCCTCAAGACTACAAGAAACCCATCCAGGAAGTGCTAAAGGAAATGACTGAT GGAGGTGTGGATTTTTCGTTTGAAGTCATCGGTCGGCTTGACACCATGATGGCTTCCCTG TTATGTTGTCATGAGGCATGTGGCACAAGCGTCATCGTAGGGGTACCTCCTGCTTCCCAG AACCTCTCAATAAACCCTATGCTGCTACTGACTGGACGCACCTGGAAGGGGGCTGTTTAT GGTGGCTTTAAGAGTAAAGAAGGTATCCCAAAACTTGTGGCTGATTTTATGGCTAAGAAG TTTTCACTGGATGCGTTAATAACCCATGTTTTACCTTTTGAAAAAATAAATGAAGGATTT GACCTGCTTCACTCTGGGAAAAGTATCCGTACCGTCCTGACGTTTTGA Clone variation with respect to NM_000668.4 143 a=>g;753 t=>c >OriGene 5' read for NM_000668 unedited
TCTTGGGATTTTGTAATCCCACTTTACTATAGGGCGGCACACACATTCGGCACGAGGCAA AGACTCACAGTCTGCTGGTGGGCAGAGAAGACAGAAACGACATGAGCACAGCAGGGAAAA GTAATCAAATGCAAAGCAGCTGTGCTATGGGAGGTAAAGAAACCCTTTTCCATTGAGGAT GTGGAGGTTGCACCTCCTAAGGCTTATGAAGTTCGCATTATTTATGGTGGCTGTAGGAAT CTGTCGCACAGATGACCACGTGGTTAGTGGCAACCTGGTGACCCCCCTTCCTGTGATTTT AGGCCATGAGGCAGCCGGCATCGTGGAGAGTGTTGGAGAAGGGGTGACTACAGTCAAACC AGGTGATAAAGTCATCCCGCTCTTTACTCCTCAGTGTGGAAAATGCAGAGTTTGTAAAAA CCCGGAGAGCAACTACTGCTTGAAAAATGATCTAGGCAATCCTCGGGGGACCCTGCAGGA TGGCACCAGGAGGTTCACCTGCAGGGGGAAGCCCATTCACCACTTCCTTGGCACCAGCAC CTTCTCCCAGTACACGGTGGTGGATGAGAATGCAGTGGCCAAAATTGATGCAGCCTCGCC CCTGGAGAAAGTCTGCCTCATTGGCTGTGGATTCTCGACTGGTTATGGGTCTGCAGTTAA CGTTGCCAATGTCACCCCANGCTCTACCTGTGCTGTGTTTGGCCTGAGGAGGGTCGGCCT ATCTGCTGTTATGGGCTGTNAAGCAGCTGGAGCAGCCAGAATCATTGCGGTGGACATNNC ACAGGGACAATTTTGCAAGGCCAAGAGTTGGGTGCCACTGAATGCATCACCCCTCAGACT ACAGGATACCATCCAGNAAGTGCTAAAGGAATGACTNGATGGAGTGTGGATTTTCGTTTG AAGTCATCGTCGGCTTGCACCATGAGG >OriGene 3' read for NM_000668 unedited
CTATGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAAGAAACACAG TAAATGATAAACTATTTTACTTTATGTCTAAGGTCTTTCATAATATGAAATAGAATGTAG ATATTGCAACAATAGCATTTTTGGAGACAGCTACCTCCTTTACCAGGAATAATCTTTGCA TGTCACATTTAGAGATAAAGCTCAAAATGCAAATCCTTCCCCTGAGAGTGGGAAAGCATT AACAAATGAGAGTGGGAAAAGCATTAACAAAGCATTAACACAGGTCTTTACATATTCAAA ATATTAAACTAATGCTAGGATTATAGACTTGATTTTAAGACATGGTAGTTAATAGAAAAG TTCTAGATTGAAAACAATTTTGCAAAAATATACATTTGTATATGTGTATATATGTATGTG TATATATATATCTACTAGGAAAATATATTGCTTAAGGTGTTTGTGCCATTTCTTAGTTAC CAATTTTACATTCTGCATTCACAGCATTTGCCATCCCATCTCTACACTTAAAGCCACCAT ATTACTTTTAATTACTTTATACACATTTTCAGTCATCATCTAAATAATCATTTATAGCCA CAAACTAATACTGTTTAGTCAATGAAGCACTTTCATCAGCAAAAATTACAGATTTTTTGA ACATGATTCTGGGAATAGTTCAGTTTTTACAATTAGTAATTGAGGCTTTGTCATATGAAG CAAGAAATCTGCTATTATTTTAAAAAATCAATTGTATATCGACCATAAAGAAATAGTATA AAAGTCATAAAAACACTGCCATTTACTGTAGTAAAATGTGGGGCATTTTATTTGAGTTCC TAAGAAATTTCCTGTATTTAGACTCTCGGCCTTCACTACTTGAAATGTGAGTCTATCCTT TACCTTACCCTAGCTTTTACCCCAGGGATTTGGTACATTTTTATTAAAAAAAGAAATTGT CGACGGTGAAACCGGACAGAGTTTGAAGACTTCATCACATGGGCCACA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000668 |
| Insert Size | 2790 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000668.3, NP_000659.2 |
| RefSeq Size | 2666 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 125 |
| UniProt ID | P00325 |
| Domains | ADH_zinc_N |
| Protein Families | Druggable Genome |
| Protein Pathways | Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism |
| Gene Summary | The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205391 | ADH1B (Myc-DDK-tagged)-Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) |
CNY 5,488.00 |
|
| RC205391L1 | Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205391L2 | Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), mGFP tagged |
CNY 5,890.00 |
|
| RC205391L3 | Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205391L4 | Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), mGFP tagged |
CNY 7,888.00 |
|
| RG205391 | ADH1B (tGFP-tagged) - Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) |
CNY 7,088.00 |
