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PDHA1 (NM_000284) Human Untagged Clone
CAT＃: SC119981
PDHA1 (untagged)-Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PDHA; PDHAD; PDHCE1A; PHE1A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119981 sequence for NM_000284 edited (data generated by NextGen Sequencing)
ATGAGGAAGATGCTCGCCGCCGTCTCCCGCGTGCTGTCTGGCGCTTCTCAGAAGCCGGCA AGCAGAGTGCTGGTAGCATCCCGTAATTTTGCAAATGATGCTACATTTGAAATTAAGAAA TGTGACCTTCACCGGCTGGAAGAAGGCCCTCCTGTCACAACAGTGCTCACCAGGGAGGAT GGGCTCAAATACTACAGGATGATGCAGACTGTACGCCGAATGGAGTTGAAAGCAGATCAG CTGTATAAACAGAAAATTATTCGTGGTTTCTGTCACTTGTGTGATGGTCAGGAAGCTTGC TGTGTGGGCCTGGAGGCCGGCATCAACCCCACAGACCATCTCATCACAGCCTACCGGGCT CACGGCTTTACTTTCACCCGGGGCCTTTCCGTCCGAGAAATTCTCGCAGAGCTTACAGGA CGAAAAGGAGGTTGTGCTAAAGGGAAAGGAGGATCGATGCACATGTATGCCAAGAACTTC TACGGGGGCAATGGCATCGTGGGAGCGCAGGTGCCCCTGGGCGCTGGGATTGCTCTAGCC TGTAAGTATAATGGAAAAGATGAGGTCTGCCTGACTTTATATGGCGATGGTGCTGCTAAC CAGGGCCAGATATTCGAAGCTTACAACATGGCAGCTTTGTGGAAATTACCTTGTATTTTC ATCTGTGAGAATAATCGCTATGGAATGGGAACGTCTGTTGAGAGAGCGGCAGCCAGCACT GATTACTACAAGAGAGGCGATTTCATTCCTGGGCTGAGAGTGGATGGAATGGATATCCTG TGCGTCCGAGAGGCAACAAGGTTTGCTGCTGCCTATTGTAGATCTGGGAAGGGGCCCATC CTGATGGAGCTGCAGACTTACCGTTACCACGGACACAGTATGAGTGACCCTGGAGTCAGT TACCGTACACGAGAAGAAATTCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTC AAGGACAGGATGGTGAACAGCAATCTTGCCAGTGTGGAAGAACTAAAGGAAATTGATGTG GAAGTGAGGAAGGAGATTGAGGATGCTGCCCAGTTTGCCACGGCCGATCCTGAGCCACCT TTGGAAGAGCTGGGCTACCACATCTACTCCAGCGACCCACCTTTTGAAGTTCGTGGTGCC AATCAGTGGATCAAGTTTAAGTCAGTCAGTTAA Clone variation with respect to NM_000284.3 >OriGene 5' read for NM_000284 unedited
GGGCACCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTCTGCTGG GGCACCTGAAGGAGACTTGGGGGCACCCGCGTCGTGCCTCCTGGGTTGTGAGGAGTCGCC GCTGCCGCCACTGCCTGTGCTTCATGAGGAAGATGCTCGCCGCCGTCTCCCGCGTGCTGT CTGGCGCTTCTCAGAAGCCGGCAAGCAGAGTGCTGGTAGCATCCCGTAATTTTGCAAATG ATGCTACATTTGAAATTAAGAAATGTGACCTTCACCGGCTGGAAGAAGGCCCTCCTGTCA CAACAGTGCTCACCAGGGAGGATGGGCTCAAATACTACAGGATGATGCAGACTGTACGCC GAATGGAGTTGAAAGCAGATCAGCTGTATAAACAGAAAATTATTCGTGGTTTCTGTCACT TGTGTGATGGTCAGGAAGCTTGCTGTGTGGGCCTGGAGGCCGGCATCAACCCCACAGACC ATCTCATCACAGCCTACCGGGCTCACGGCTTTACTTTCACCCGGGGCCTTTCCGTCCGAG AAATTCTCGCAGAGCTTACAGGACGAAAAGGAGGTTGTGCTAAAGGGAAAGGAGGATCGA TGCACATGTATGCCAAGAACTTCTACGGNGGCAATGGCATCGTGGGAGCGCAGGTGCCCC TGNGCGCTGGGATTGCTCTAGCCCTGTAGTATAATGGAAAAGATGAGGTCTGCCTGACTN TATATGGCGATGGTGCTGCTAACCAGNGCCAGATATTCGAAGCTTACAACATGGCAGCCT TTGTGGAAATTACCTTGTATTNTCATCTGTGAGAATAATCGCTATGGAATGGGAACGTCT GTTGAGAGAGCGGCAGCCAGCACTGATTACTANCAGAGAGGGCGATTCATTCCTGGGCTG AGAGTGGATGGAATGGATATCCTGT >OriGene 3' read for NM_000284 unedited
TTGGACCGCGGCCGCTTCTATAGTCGGTTTTTTTTTTTTTTTTTTAATCTTTAAGCACTC AATAATTCATCTTTTAATGCACTAATGTACAAACTGCATGCAATTACTACCTGCTCAATC TACACACTTAATGGAAGTTTCCAAGAATTTCATTGAATTTCATTGACTGGGTTTTCTTCC TCCTTAACCAAGTTGAGAACACTGTCTGGTAGCCCCCTGAAGGTATAACCTCTCCTTCTC CTCCCCTTAACTGACTGACTTAAACTTGATCCACTGATTGGCACCACGAACTTCAAAAGG TGGGTCGCTGGAGTAGATGTGGTAGCCCAGCTCTTCCAAAGGTGGCTCAGGATCGGCCGT GGCAAACTGGGCAGCATCCTCAATCTCCTTCCTCACTTCCACATCAATTTCCTTTAGTTC TTCCACACTGGCAAGATTGCTGTTCACCATCCTGTCCTTGAGAAGCATAATAAGGTCACT CTTACTTCTTACTTCCTGAATTTCTTCTCGTGTACGGTAACTGACTCCAGGGTCACTCAT ACTGTGTCCGTGGGAACGGTAAGTCTGCAACTCCATAAGAATGGCCCCTTTTCCCAATTT ACATTGGGCGCCAGCAAACCTTTGTTGCCCTTTGGGCCCCCAAGGAATCCTTTCCTTCCC CTTTAAACCCAAAAAGAAAAACCCCTCCTTTGTGGAAAAAAAGGGGGGGGGGGCGCTTTT TTAAAAAAAATGTCCCCTTCTTATAAGATTTTTTTCCCCAATAAAAAAAAAGGGGGTTTT CCCCCAACGCCCCGCCGGGGGAGGCCTAAAATTTTGGGCCGGGGTGGAACCCCCCCCCCC TCTTTTTTAATGAGGGGGACCATTTTTTTTTTTTTTTTTTTTTGGGCGGAGAGAAAAANC CCCCCGGGGGGGGGGGCGGTCTCTCCAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000284 |
| Insert Size | 1460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000284.1, NP_000275.1 |
| RefSeq Size | 1472 bp |
| RefSeq ORF | 1173 bp |
| Locus ID | 5160 |
| UniProt ID | P08559 |
| Domains | E1_dehydrog |
| Protein Families | Druggable Genome |
| Protein Pathways | Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis |
| Gene Summary | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
,Chu, J;Haynes, RD;Corbel, SY;Li, P;González-González, E;Burg, JS;Ataie, NJ;Lam, AJ;Cranfill, PJ;Baird, MA;Davidson, MW;Ng, HL;Garcia, KC;Contag, CH;Shen, K;Blau, HM;Lin, MZ;,
Nat. Methods
,PubMed ID 24633408
[PDHA1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201831 | PDHA1 (Myc-DDK-tagged)-Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 3,656.00 |
|
| RC201831L1 | Lenti ORF clone of Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201831L2 | Lenti ORF clone of Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201831L3 | Lenti ORF clone of Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201831L4 | Lenti ORF clone of Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG201831 | PDHA1 (tGFP-tagged) - Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,256.00 |
