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MSH2 (NM_000251) Human Untagged Clone
CAT＃: SC119995
MSH2 (untagged)-Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2)
CNY 10,272.00
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MMRCS2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119995 sequence for NM_000251 edited (data generated by NextGen Sequencing)
ATGGCGGTGCAGCCGAAGGAGACGCTGCAGTTGGAGAGCGCGGCCGAGGTCGGCTTCGTG CGCTTCTTTCAGGGCATGCCGGAGAAGCCGACCACCACAGTGCGCCTTTTCGACCGGGGC GACTTCTATACGGCGCACGGCGAGGACGCGCTGCTGGCCGCCCGGGAGGTGTTCAAGACC CAGGGGGTGATCAAGTACATGGGGCCGGCAGGAGCAAAGAATCTGCAGAGTGTTGTGCTT AGTAAAATGAATTTTGAATCTTTTGTAAAAGATCTTCTTCTGGTTCGTCAGTATAGAGTT GAAGTTTATAAGAATAGAGCTGGAAATAAGGCATCCAAGGAGAATGATTGGTATTTGGCA TATAAGGCTTCTCCTGGCAATCTCTCTCAGTTTGAAGACATTCTCTTTGGTAACAATGAT ATGTCAGCTTCCATTGGTGTTGTGGGTGTTAAAATGTCCGCAGTTGATGGCCAGAGACAG GTTGGAGTTGGGTATGTGGATTCCATACAGAGGAAACTAGGACTGTGTGAATTCCCTGAT AATGATCAGTTCTCCAATCTTGAGGCTCTCCTCATCCAGATTGGACCAAAGGAATGTGTT TTACCCGGAGGAGAGACTGCTGGAGACATGGGGAAACTGAGACAGATAATTCAAAGAGGA GGAATTCTGATCACAGAAAGAAAAAAAGCTGACTTTTCCACAAAAGACATTTATCAGGAC CTCAACCGGTTGTTGAAAGGCAAAAAGGGAGAGCAGATGAATAGTGCTGTATTGCCAGAA ATGGAGAATCAGGTTGCAGTTTCATCACTGTCTGCGGTAATCAAGTTTTTAGAACTCTTA TCAGATGATTCCAACTTTGGACAGTTTGAACTGACTACTTTTGACTTCAGCCAGTATATG AAATTGGATATTGCAGCAGTCAGAGCCCTTAACCTTTTTCAGGGTTCTGTTGAAGATACC ACTGGCTCTCAGTCTCTGGCTGCCTTGCTGAATAAGTGTAAAACCCCTCAAGGACAAAGA CTTGTTAACCAGTGGATTAAGCAGCCTCTCATGGATAAGAACAGAATAGAGGAGAGATTG AATTTAGTGGAAGCTTTTGTAGAAGATGCAGAATTGAGGCAGACTTTACAAGAAGATTTA CTTCGTCGATTCCCAGATCTTAACCGACTTGCCAAGAAGTTTCAAAGACAAGCAGCAAAC TTACAAGATTGTTACCGACTCTATCAGGGTATAAATCAACTACCTAATGTTATACAGGCT CTGGAAAAACATGAAGGAAAACACCAGAAATTATTGTTGGCAGTTTTTGTGACTCCTCTT ACTGATCTTCGTTCTGACTTCTCCAAGTTTCAGGAAATGATAGAANCAACTTTAGATATG GATCAGGTGGAAAACCATGAATTCCTTGTAAAACCTTCATTTGATCCTAATCTCAGTGAA TTAAGAGAAATAATGAATGACTTGGAAAAGAAGATGCAGTCAACATTAATAAGTGCAGCC AGAGATCTTGGCTTGGACCCTGGCAAACAGATTAAACTGGATTCCAGTGCACAGTTTGGA TATTACTTTCGTGTAACCTGTAAGGAAGAAAAAGTCCTTCGTAACAATAAAAACTTTAGT ACTGTAGATATCCAGAAGAATGGTGTTAAATTTACCAACAGCAAATTGACTTCTTTAAAT GAAGAGTATACCAAAAATAAAACAGAATATGAAGAAGCCCAGGATGCCATTGTTAAAGAA ATTGTCAATATTTCTTCAGGCTATGTAGAACCAATGCAGACACTCAATGATGTGTTAGCT CAGCTAGATGCTGTTGTCAGCTTTGCTCACGTGTCAAATGGAGCACCTGTTCCATATGTA CGACCAGCCATTTTGGAGAAAGGACAAGGAAGAATTATATTAAAAGCATCCAGGCATGCT TGTGTTGAAGTTCAAGATGAAATTGCATTTATTCCTAATGACGTATACTTTGAAAAAGAT AAACAGATGTTCCACATCATTACTGGCCCCAATATGGGAGGTAAATCAACATATATTCGA CAAACTGGGGTGATAGTACTCATGGCCCAAATTGGGTGTTTTGTGCCATGTGAGTCAGCA GAAGTGTCCATTGTGGACTGCATCTTAGCCCGAGTAGGGGCTGGTGACAGTCAATTGAAA GGAGTCTCCACGTTCATGGCTGAAATGTTGGAAACTGCTTCTATCCTCAGGTCTGCAACC AAAGATTCATTAATAATCATAGATGAATTGGGAAGAGGAACTTCTACCTACGATGGATTT GGGTTAGCATGGGCTATATCAGAATACATTGCAACAAAGATTGGTGCTTTTTGCATGTTT GCAACCCATTTTCATGAACTTACTGCCTTGGCCAATCAGATACCAACTGTTAATAATCTA CATGTCACAGCACTCACCACTGAAGAGACCTTAACTATGCTTTATCAGGTGAAGAAAGGT GTCTGTGATCAAAGTTTTGGGATTCATGTTGCAGAGCTTGCTAATTTCCCTAAGCATGTA ATAGAGTGTGCTAAACAGAAAGCCCTGGAACTTGAGGAGTTTCAGTATATTGGAGAATCG CAAGGATATGATATCATGGAACCAGCAGCAAAGAAGTGCTATCTGGAAAGAGAGCAAGGT GAAAAAATTATTCAGGAGTTCCTGTCCAAGGTGAAACAAATGCCCTTTACTGAAATGTCA GAAGAAAACATCACAATAAAGTTAAAACAGCTAAAAGCTGAAGTAATAGCAAAGAATAAT AGCTTTGTAAATGAAATCATTTCACGAATAAAAGTTACTACGTGA Clone variation with respect to NM_000251.1 1366 a=>n >OriGene 5' read for NM_000251 unedited
TGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGGAGGTTTCGACATGGC GGTGCAGCCGAAGGAGACGCTGCAGTTGGAGAGCGCGGCCGAGGTCGGCTTCGTGCGCTT CTTTCAGGGCATGCCGGAGAAGCCGACCACCACAGTGCGCCTTTTCGACCGGGGCGACTT CTATACGGCGCACGGCGAGGACGCGCTGCTGGCCGCCCGGGAGGTGTTCAAGACCCAGGG GGTGATCAAGTACATGGGGCCGGCAGGAGCAAAGAATCTGCAGAGTGTTGTGCTTAGTAA AATGAATTTTGAATCTTTTGTAAAAGATCTTCTTCTGGTTCGTCAGTATAGAGTTGAAGT TTATAAGAATAGAGCTGGAAATAAGGCATCCAAGGAGAATGATTGGTATTTGGCATATAA GGCTTCTCCTGGCAATCTCTCTCAGTTTGAAGACATTCTCTTTGGTAACAATGATATGTC AGCTTCCATTGGTGTTGTGGGTGTTAAAATGTCCGCAGTTGATGGCCAGAGACAGGTTGG AGTTGGGTATGTGGATTCCATACAGAGGAAACTAGGACTGTGTGAATTCCCTGATAATGA TCAGTTCTCCAATCTTGAGGCTCTCCTCATCCAGATTGGACCAAGGAATGTGTTTTACCC GGAGGAGAGACTGCTGGAGACATGGNGAAACTGAGACAGATAATTAAAGAGGAGGAATTC TGATCACAGAAGAANAAAGCTGACTNTTCACAAAGACATTATCAGACCTCACCCGNNTGT GAAGGCAAAGGGAGAGCAATGATAGTGCTNGATGCCAGATGGAGAATCAGGTGCAGTTCA NACTGGCTGCGGNATCAGTTTTAGACTCTATAGATGATCCACTTGGGACGTTGAACTGCT ACTTTGACTCGCCGNTATGATTGGATGGCGCNTCAGCCTAG >OriGene 3' read for NM_000251 unedited
CTCTGCATTTTTTTATAAAATTCAGCACATCACTTATTATTGCCTATGTCAATTGCAAAC AGTCCTCAGTTACAGCTCTCATTTTTCAAAATAAAAATCTTTGAAAATGTCCTCAAAGTA AAATATTACTAAATATCTTATTAAGTTGATAGCCCATGGGCACTGACAGTTAACACTATG GAAAAAGGGTTAATATAAAACAATATAAAACTATTACAGACAATAGCTTATCAATATTAC CTTCATTCCATTACTGGGATTTTTCACGTAGTAACTTTTATTCGTGAAATGATTTCATTT ACAAAGCTATTATTCTTTGCTATTACTTCAGCTTTTAGCTGTTTTAACTTTATTGTGATG TTTTCTTCTGACATTTCAGTAAAGGGCATTTGTTTCACCTTGGACAGGAACTCCTGAATA ATTTTTTCACCTTGCTCTCTTTCCAGATAGCACTTCTTTGCTGCTGGTTCCATGATATCA TATCCTTGCGATTCTCCAATATACTGAAACTCCTCAAGTTCCAGGGCTTTCTGTTTAGCA CACTCTATTACATGCTTAGGGAAATTAGCAAGCTCTGCAACATGAATCCCAAAACTTTGA TCACAGACACCTTTCTTCACCTGATAAAGCATAGTTAAGGTCTCTTCAGTGGTGAGTGCT GTGACATGTAGATTATTAACAGTTGGTATCTGATTGGCCAAGGCAGTAAGTTCATGACAA TGGGTTGCAAACATGCANAAAGCACCAAATCTTGTTGCAATGTATTCTGATATAGCCCAT GCTAACCAAATNCATCGTAGGTAGAAAGTTCTCTTCCAANTCATCTATGATTATTAATGA AATCTTTGGTGCAGACCTGAGGATAGAAGCAGTTTCCAACATTTCACCATGAACCGTGAG ACTNCTTTCAATNGACTGTTCACAGCCCCTACTCGGGCTAAGATGCAGTCACAATGGACA CCTCTGCTGACTAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000251 |
| Insert Size | 3660 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000251.1, NP_000242.1 |
| RefSeq Size | 3145 bp |
| RefSeq ORF | 2805 bp |
| Locus ID | 4436 |
| UniProt ID | P43246 |
| Domains | MutS_V, MutS_I, MutS_III, MutS_II, MutS_IV |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Colorectal cancer, Mismatch repair, Pathways in cancer |
| Gene Summary | This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205848 | MSH2 (Myc-DDK-tagged)-Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2) |
CNY 6,840.00 |
|
| RC205848L1 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), Myc-DDK-tagged |
CNY 9,240.00 |
|
| RC205848L2 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), mGFP tagged |
CNY 5,890.00 |
|
| RC205848L3 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205848L4 | Lenti ORF clone of Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2), mGFP tagged |
CNY 9,240.00 |
|
| RG205848 | MSH2 (tGFP-tagged) - Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2) |
CNY 8,440.00 |
