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Lysosomal acid lipase (LIPA) (NM_000235) Human Untagged Clone
CAT＃: SC120024
LIPA (untagged)-Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CESD; LAL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120024 sequence for NM_000235 edited (data generated by NextGen Sequencing)
ATGAAAATGCGGTTCTTGGGGTTGGTGGTCTGTTTGGTTCTCTGGACCCTGCATTCTGAG GGGTCTAGAGGGAAACTGACAGCTGTGGATCCTGAAACAAACATGAATGTGAGTGAAATT ATCTCTTACTGGGGATTCCCTAGTGAGGAATACCTAGTTGAGACAGAAGATGGATATATT CTGTGCCTTAACCGAATTCCTCATGGGAGGAAGAACCATTCTGACAAAGGTCCCAAACCA GTTGTCTTCCTGCAACATGGCTTGCTGGCAGATTCTAGTAACTGGGTCACAAACCTTGCC AACAGCAGCCTGGGCTTCATTCTTGCTGATGCTGGTTTTGACGTGTGGATGGGCAACAGC AGAGGAAATACCTGGTCTCGGAAACATAAGACACTCTCAGTTTCTCAGGATGAATTCTGG GCTTTCAGTTATGATGAGATGGCAAAATATGACCTACCAGCTTCCATTAACTTCATTCTG AATAAAACTGGCCAAGAACAAGTGTATTATGTGGGTCATTCTCAAGGCACCACTATAGGT TTTATAGCATTTTCACAGATCCCTGAGCTGGCTAAAAGGATTAAAATGTTTTTTGCCCTG GGTCCTGTGGCTTCCGTCGCCTTCTGTACTAGCCCTATGGCCAAATTAGGACGATTACCA GATCATCTCATTAAGGACTTATTTGGAGACAAAGAATTTCTTCCCCAGAGTGCGTTTTTG AAGTGGCTGGGTACCCACGTTTGCACTCATGTCATACTGAAGGAGCTCTGTGGAAATCTC TGTTTTCTTCTGTGTGGATTTAATGAGAGAAATTTAAATATGTCTAGAGTGGATGTATAT ACAACACATTCTCCTGCTGGAACTTCTGTGCAAAACATGTTACACTGGAGCCAGGCTGTT AAATTCCAAAAGTTTCAAGCCTTTGACTGGGGAAGCAGTGCCAAGAATTATTTTCATTAC AACCAGAGTTATCCTCCCACATACAATGTGAAGGACATGCTTGTGCCGACTGCAGTCTGG AGCGGGGGTCACGACTGGCTTGCAGATGTCTACGACGTCAATATCTTACTGACTCAGATC ACCAACTTGGTGTTCCATGAGAGCATTCCGGAATGGGAGCATCTTGACTTCATTTGGGGC CTGGATGCCCCTTGGAGGCTTTATAATAAAATTATTAATCTAATGAGGAAATATCAGTGA Clone variation with respect to NM_000235.2 67 g=>a >OriGene 5' read for NM_000235 unedited
CTTTTGTATACGACTAACTATAGGCGGCCGCGATTCGCCGAGCGCGGCCCGGCAGACACT CCGATGAAAAGCGGTTCTTGGGGTTGGTGGTCTGTTTGGTTCTCTGGACCCTGCATTCTG AGGGGTCTAGAGGGAAACTGACAGCTGTGGATCCTGAAACAAACATGAATGTGAGTGAAA TTATCTCTTACTGGGGATTCCCTAGTGAGGACATACCTAGTTGAGACAGAAGATGGATAT ATTCTGTGCCTTAACCGAATTCCTCATGGGAGGAAGAACCATTCTGACAAAGGTCCCAAA CCAGTTGTCTTCCTGCAACATGGCTTGCTGGCAGATTCTAGTAACTGGGTCACAAACCTT GCCAACAGCAGCCTGGGCTTCATTCTTGCTGATGCTGGTTTTGACGTGTGGATGGGCAAC AGCAGAGGAAATACCTGGTCTCGGAAACATAAGACACTCTCAGTTTCTCAGGATGAATTC TGGGCTTTCAGTTATGATGAGATGGCAAAATATGACCTACCAGCTTCCATTAACTTCATT CTGAATAAAACTGGCCAAGAACAAGTGTATTATGTGGGTCATTCTCAAGGCACCACTATA GGTTTTATAGCATTTTCACAGATCCCTGAGCTGGCTAAAAGGATTAAAATGTTTTTTGCC CTGNGTCCTGTGGCTTCCGTCGCCTTCTGTACTAGCCCTATGGCCAAATTANGACGATTA CCAGATCATCTCATTAAGGACTTATTTGGAGACAAAGAATTTCTTCCCCAGAGTGCNGTN TTGAAGTGGCTGGGTACCCACGTTTGCACTCATGTCATACTGAAGGAGCTCTGTGGAAAT CTCTGTTTCCTCCTGTGTGGATTAATGAGAGGAATTTAATATGTCTAGAGTGATGTATAT ACACACATTCTCTGCTGGAACTNCTGTGCAAACATGTACACTGGAGCCAGCTGTNTAATT CCAAGTTTCAGCCTTTGC >OriGene 3' read for NM_000235 unedited
NNTTTTTTGCTTTGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTG CCTATAAGTCTCACTCAATTTTATTGAAAAGTTTAAGACTTTAAAAGTTACAAAGTAGTA TTCGCCCTGCCTATATTAACCATTAACAAGCATTGTACTGTTAGTGCAGCTAAAGTAATT ATATCAGAAAAACATATCAATTGTCATTTTGTCCACCGGACAGTATTGTAAGGAAATGAT GCTGCAAGAAAAGCAGACAAATATGGACTTCCCAACATAGGGACGTGAAGCAGGTGTATT AAAAATAAGGATGCATATAAAACTAAGACACAAAATGAATCCCTGAGCTGAGTTTGCATC CGAAAACATCACGTTCACACTTCATTTGGGTGAAAATGAACAAGGACACTTCTCATGGGC AACCACGGGGCTTTGGCTTCACACAGATGTGATCAATAACGACAGTAGCGGGCTCCAAGA GTACATTAATCTCAGAAAAAATAGTGGTATAGTCTCCACAGGGATTTGCTTCTCAGATAA GTAGAACTATGGCCTGCCATGAAATTGAGAGCTCATGAATATAGATGGTTGAGTGTATCA GTCTTCAAAGCATTTAAAACATACTTTTGTTTTTGTTTTATCAGTGCAATTTGTTTTTGA AGACGCCGGANAACTATTTCATTTAACCTGAGCAATTAAAACGTGTCAAATCACAGCTGT CACAGTTAGGTTTGATAAAATTAATTGTTAGCATCCTTATTACTGGCACAGCTGTGATAT TTCTGGATTTGACTATGCACCTGCTGCNAGAAACATGATAAATGTCATTTAGATCTCTAC TACAAGACCGTTAGACTAGAGAGCTCTTTAAGGCAGGACATGAAACTGCGTNACTTTATA CTCGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000235 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000235.2, NP_000226.2 |
| RefSeq Size | 2654 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 3988 |
| UniProt ID | P38571 |
| Domains | abhydrolase, abhydro_lipase |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome, Steroid biosynthesis |
| Gene Summary | This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) lacks an internal segment in the 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201637 | LIPA (Myc-DDK-tagged)-Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2 |
CNY 5,488.00 |
|
| RC201637L1 | Lenti ORF clone of Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201637L2 | Lenti ORF clone of Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2, mGFP tagged |
CNY 7,888.00 |
|
| RC201637L3 | Lenti ORF clone of Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201637L4 | Lenti ORF clone of Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201637 | LIPA (tGFP-tagged) - Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2 |
CNY 7,088.00 |
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