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MSH6 (NM_000179) Human Untagged Clone
CAT＃: SC120035
MSH6 (untagged)-Human mutS homolog 6 (E. coli) (MSH6)
CNY 13,968.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GTBP; GTMBP; HNPCC5; HSAP; MMRCS3; p160 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120035 sequence for NM_000179 edited (data generated by NextGen Sequencing)
ATGTCGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCCGGCGCTGAGTGATGCC AACAAGGCCTCGGCCAGGGCCTCACGCGAAGGCGGCCGTGCCGCCGCTGCCCCCGGGGCC TCTCCTTCCCCAGGCGGGGATGCGGCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTG GCGCGCTCCGCGTCACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTA GCGCCTGCTGCCCCCACCAGTTGTGACTTCTCACCAGGAGATTTGGTTTGGGCCAAGATG GAGGGTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTTTGATGGAACATTCATC CGCGAGAAAGGGAAATCAGTCCGTGTTCATGTACAGTTTTTTGATGACAGCCCAACAAGG GGCTGGGTTAGCAAAAGGCTTTTAAAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAG AAGGGAGGTCATTTTTACAGTGCAAAGCCTGAAATACTGAGAGCAATGCAACGTGCAGAT GAAGCCTTAAATAAAGACAAGATTAAGAGGCTTGAATTGGCAGTTTGTGATGAGCCCTCA GAGCCAGAAGAGGAAGAAGAGATGGAGGTAGGCACAACTTACGTAACAGATAAGAGTGAA GAAGATAATGAAATTGAGAGTGAAGAGGAAGTACAGCCTAAGACACAAGGATCTAGGCGA AGTAGCCGCCAAATAAAAAAACGAAGGGTCATATCAGATTCTGAGAGTGACATTGGTGGC TCTGATGTGGAATTTAAGCCAGACACTAAGGAGGAAGGAAGCAGTGATGAAATAAGCAGT GGAGTGGGGGATAGTGAGAGTGAAGGCCTGAACAGCCCTGTCAAAGTTGCTCGAAAGCGG AAGAGAATGGTGACTGGAAATGGCTCTCTTAAAAGGAAAAGCTCTAGGAAGGAAACGCCC TCAGCCACCAAACAAGCAACTAGCATTTCATCAGAAACCAAGAATACTTTGAGAGCTTTC TCTGCCCCTCAAAATTCTGAATCCCAAGCCCACGTTAGTGGAGGTGGTGATGACAGTAGT CGCCCTACTGTTTGGTATCATGAAACTTTAGAATGGCTTAAGGAGGAAAAGAGAAGAGAT GAGCACAGGAGGAGGCCTGATCACCCCGATTTTGATGCATCTACACTCTATGTGCCTGAG GATTTCCTCAATTCTTGTACTCCTGGGATGAGGAAGTGGTGGCAGATTAAGTCTCAGAAC TTTGATCTTGTCATCTGTTACAAGGTGGGGAAATTTTATGAGCTGTACCACATGGATGCT CTTATTGGAGTCAGTGAACTGGGGCTGGTATTCATGAAAGGCAACTGGGCCCATTCTGGC TTTCCTGAAATTGCATTTGGCCGTTATTCAGATTCCCTGGTGCAGAAGGGCTATAAAGTA GCACGAGTGGAACAGACTGAGACTCCAGAAATGATGGAGGCACGATGTAGAAAGATGGCA CATATATCCAAGTATGATAGAGTGGTGAGGAGGGAGATCTGTAGGATCATTACCAAGGGT ACACAGACTTACAGTGTGCTGGAAGGTGATCCCTCTGAGAACTACAGTAAGTATCTTCTT AGCCTCAAAGAAAAAGAGGAAGATTCTTCTGGCCATACTCGTGCATATGGTGTGTGCTTT GTTGATACTTCACTGGGAAAGTTTTTCATAGGTCAGTTTTCAGATGATCGCCATTGTTCG AGATTTAGGACTCTAGTGGCACACTATCCCCCAGTACAAGTTTTATTTGAAAAAGGAAAT CTCTCAAAGGAAACTAAAACAATTCTAAAGAGTTCATTGTCCTGTTCTCTTCAGGAAGGT CTGATACCCGGCTCCCAGTTTTGGGATGCATCCAAAACTTTGAGAACTCTCCTTGAGGAA GAATATTTTAGGGAAAAGCTAAGTGATGGCATTGGGGTGATGTTACCCCAGGTGCTTAAA GGTATGACTTCAGAGTCTGATTCCATTGGGTTGACACCAGGAGAGAAAAGTGAATTGGCC CTCTCTGCTCTAGGTGGTTGTGTCTTCTACCTCAAAAAATGCCTTATTGATCAGGAGCTT TTATCAATGGCTAATTTTGAAGAATATATTCCCTTGGATTCTGACACAGTCAGCACTACA AGATCTGGTGCTATCTTCACCAAAGCCTATCAACGAATGGTGCTAGATGCAGTGACATTA AACAACTTGGAGATTTTTCTGAATGGAACAAATGGTTCTACTGAAGGAACCCTACTAGAG AGGGTTGATACTTGCCATACTCCTTTTGGTAAGCGGCTCCTAAAGCAATGGCTTTGTGCC CCACTCTGTAACCATTATGCTATTAATGATCGTCTAGATGCCATAGAAGACCTCATGGTT GTGCCTGACAAAATCTCCGAAGTTGTAGAGCTTCTAAAGAAGCTTCCAGATCTTGAGAGG CTACTCAGTAAAATTCATAATGTTGGGTCTCCCCTGAAGAGTCAGAACCACCCAGACAGC AGGGCTATAATGTATGAAGAAACTACATACAGCAAGAAGAAGATTATTGATTTTCTTTCT GCTCTGGAAGGATTCAAAGTAATGTGTAAAATTATAGGGATCATGGAAGAAGTTGCTGAT GGTTTTAAGTCTAAAATCCTTAAGCAGGTCATCTCTCTGCAGACAAAAAATCCTGAAGGT CGTTTTCCTGATTTGACTGTAGAATTGAACCGATGGGATACAGCCTTTGACCATGAAAAG GCTCGAAAGACTGGACTTATTACTCCCAAAGCAGGCTTTGACTCTGATTATGACCAAGCT CTTGCTGACATAAGAGAAAATGAACAGAGCCTCCTGGAATACCTAGAGAAACAGCGCAAC AGAATTGGCTGTAGGACCATAGTCTATTGGGGGATTGGTAGGAACCGTTACCAGCTGGAA ATTCCTGAGAATTTCACCACTCGCAATTTGCCAGAAGAATACGAGTTGAAATCTACCAAG AAGGGCTGTAAACGATACTGGACCAAAACTATTGAAAAGAAGTTGGCTAATCTCATAAAT GCTGAAGAACGGAGGGATGTATCATTGAAGGACTGCATGCGGCGACTGTTCTATAACTTT GATAAAAATTACAAGGACTGGCAGTCTGCTGTAGAGTGTATCGCAGTGTTGGATGTTTTA CTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGGTCCTATGTGTCGCCCAGTAATTCTG TTGCCGGAAGATACCCCCCCCTTCTTAGAGCTTAAAGGATCACGCCATCCTTGCATTACG AAGACTTTTTTTGGAGATGATTTTATTCCTAATGACATTCTAATAGGCTGTGAGGAAGAG GAGCAGGAAAATGGCAAAGCCTATTGTGTGCTTGTTACTGGACCAAATATGGGGGGCAAG TCTACGCTTATGAGACAGGCTGGCTTATTAGCTGTAATGGCCCAGATGGGTTGTTACGTC CCTGCTGAAGTGTGCAGGCTCACACCAATTGATAGAGTGTTTACTAGACTTGGTGCCTCA GACAGAATAATGTCAGGTGAAAGTACATTTTTTGTTGAATTAAGTGAAACTGCCAGCATA CTCATGCATGCAACAGCACATTCTCTGGTGCTTGTGGATGAATTAGGAAGAGGTACTGCA ACATTTGATGGGACGGCAATAGCAAATGCAGTTGTTAAAGAACTTGCTGAGACTATAAAA TGTCGTACATTATTTTCAACTCACTACCATTCATTAGTAGAAGATTATTCTCAAAATGTT GCTGTGCGCCTAGGACATATGGCATGCATGGTAGAAAATGAATGTGAAGACCCCAGCCAG GAGACTATTACGTTCCTCTATAAATTCATTAAGGGAGCTTGTCCTAAAAGCTATGGCTTT AATGCAGCAAGGCTTGCTAATCTCCCAGAGGAAGTTATTCAAAAGGGACATAGAAAAGCA AGAGAATTTGAGAAGATGAATCAGTCACTACGATTATTTCGGGAAGTTTGCCTGGCTAGT GAAAGGTCAACTGTAGATGCTGAAGCTGTCCATAAATTGCTGACTTTGATTAAGGAATTA TAG Clone variation with respect to NM_000179.2 >OriGene 5' read for NM_000179 unedited
TTGGATTTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGCGCGGTAGAT GCGGTGCTTTTAGGAGCTCCGTCCGACAGAACGGTTGGGCCTTGCCGGCTGTCGGTATGT CGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCCGGCGCTGAGTGATGCCAACA AGGCCTCGGCCAGGGCCTCACGCGAAGGCGGCCGTGCCGCCGCTGCCCCCGGGGCCTCTC CTTCCCCAGGCGGGGATGCGGCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTGGCGC GCTCCGCGTCACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTAGCGC CTGCTGCCCCCACCAGTTGTGACTTCTCACCAGGAGATTTGGTTTGGGCCAAGATGGAGG GTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTTTGATGGAACATTCATCCGCG AGAAAGGGAAATCAGTCCGTGTTCATGTACAGTTTTTTGATGACAGCCCAACAAGGGGCT GGGTTAGCAAAAGGCTTTTAAAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAGAAGG GAGGTCATTTTTACAGTGCANAGCCTGANATACTGAGAGCAATGCAACGTGCAGATGAAG CCTTAATAAAGACAGATTAAGAGGCTTNGATTGGCAGTTTGTGATGAGCCCTCAGAGCCA GAAGAGGAAGAAGAGATGGANGTAGGGCACACTTTACGTACAGATAAGAGTGAAGAAAGA TATGAAATTGAGAGTGAAGAGGAAGTACAGCCTAAGACACAGGGATCTAGCGAAGTAGCC GCCAAATAAAAAAACGAAGGGTCATATCAGATTCTGAGAGTGACATGGNTGGCTCTGATG TGGAATTTAAGCCCAGAACCTAAGGAGGAAAGAN >OriGene 3' read for NM_000179 unedited
CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTTTAAAAAATAAAG TTTATTAGATCATAATGTTGTCTGAATTTACCACCTTTGTCAGAAGTCAACTCAAAGCTT CCAATGTAGTCAGTCTATAATTCCTTAATCAAAGTCAGCAATTTATGGACAGCTTCAGCA TCTACAGTTGACCTTTCACTAGCCAGGCAAACTTCCCGAAATAATCGTAGTGACTGATTC ATCTTCTCAAATTCTCTTGCTTTTCTATGTCCCTTTTGAATAACTTCCTCTGGGAGATTA GCAAGCCTTGCTGCATTAAAGCCATAGCTTTTAGGACAAGCTCCCTTAATGAATTTATAG AGGAACGTAATAGTCTCCTGGCTGGGGTCTTCACATTCATTTTCTACCATGCATGCCATA TGTCCTAGGCGCACAGCAACATTTTGAGAATAATCTTCTACTAATGAATGGTAGTGAGTT GAAAATAATGTACGACATTTTATAGTCTCAGCAAGTTCTTTAACAACTGCATTTGCTATT GCCGTCCCATCAAATGTTGCAGTACCTCTTCCTAATTCATCCACAAGCACCAGAGAATGT GCTGTTGCATGCATGAGTATGCTGGCAGTTTCACTTAATTCAACAAAAAATGTACTTTCA CCTGACATTATTCTGTCTGAGGCACCAAGTCTAGTAAACACTCTATCAATTGGTGTGAGC CTGCACACTTCAGCAGGGACGTAACAACCCATCTGGGCCATTACAGCTAATAAGCCAGCC TGTCTCATAAGCGTAGACTTGCCCCNCATATTTGGTCCAGTAACAAGCACACATAGGGCT TGCCAATTTCCTGCTCCTCTTCTCACAGNCTATTAGAATGTCATTAGGATAAAATCATCT CCAAAAAAGTCTTGTATGCAGGATGCGGGATCTTTAGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000179 |
| Insert Size | 4310 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000179.1, NP_000170.1 |
| RefSeq Size | 4264 bp |
| RefSeq ORF | 4083 bp |
| Locus ID | 2956 |
| UniProt ID | P52701 |
| Domains | PWWP, MutS_V, MutS_I, MutS_III, MutS_II, MutS_IV |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Colorectal cancer, Mismatch repair, Pathways in cancer |
| Gene Summary | This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Mismatch-repair protein MSH6 is associated with Ku70 and regulates DNA double-strand break repair
,Ankita Shahi, Jung-Hee Lee, Yoonsung Kang, Sung Haeng Lee, Jin-Won Hyun, In-Youb Chang, Jae-Yeoul Jun, and Ho Jin You,
Nucleic Acids Res., Nov 2010; 10.1093/nar/gkq1095
[MSH6]
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Modulation of mismatch repair and genomic stability by miR-155
,Nicola Valeri, Pierluigi Gasparini, Muller Fabbri, Chiara Braconi, Angelo Veronese, Francesca Lovat, Brett Adair, Ivan Vannini, Francesca Fanini, Arianna Bottoni, Stefan Costinean, Sukhinder K. Sandhu, Gerard J Nuovo, Hansjuerg Alder, Roberta Gafa, Federica Calore, Manuela Ferracin, Giovanni Lanza, Stefano Volinia, Massimo Negrini, Michael A. McIlhatton, Dino Amadori, Richard Fishel, and Carlo M. Croce,
PNAS, Apr 2010; 107: 6982 - 6987
[MSH6]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202469 | MSH6 (Myc-DDK-tagged)-Human mutS homolog 6 (E. coli) (MSH6) |
CNY 13,960.00 |
|
| RC202469L1 | Lenti-ORF clone of MSH6 (Myc-DDK-tagged)-Human mutS homolog 6 (E. coli) (MSH6) |
CNY 13,490.00 |
|
| RC202469L2 | Lenti-ORF clone of MSH6 (mGFP-tagged)-Human mutS homolog 6 (E. coli) (MSH6) |
CNY 13,490.00 |
|
| RC202469L3 | Lenti-ORF clone of MSH6 (Myc-DDK-tagged)-Human mutS homolog 6 (E. coli) (MSH6) |
CNY 13,490.00 |
|
| RC202469L4 | Lenti-ORF clone of MSH6 (mGFP-tagged)-Human mutS homolog 6 (E. coli) (MSH6) |
CNY 16,360.00 |
|
| RG202469 | MSH6 (tGFP-tagged) - Human mutS homolog 6 (E. coli) (MSH6) |
CNY 15,560.00 |
|
| SC320735 | MSH6 (untagged)-Human mutS homolog 6 (E. coli) (MSH6) |
CNY 13,968.00 |
