DAAM1 (NM_014992) Human Untagged Clone
CAT#: SC120568
DAAM1 (untagged)-Human dishevelled associated activator of morphogenesis 1 (DAAM1)
CNY 7,512.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC120568 sequence for NM_014992 edited (data generated by NextGen Sequencing)
ATGGCCCCAAGAAAGAGAGGTGGACGAGGTATTTCATTCATCTTTTGCTGTTTCCGAAAT AATGATCACCCAGAAATCACGTATCGGCTGCGAAATGATAGCAACTTTGCGCTTCAGACC ATGGAACCAGCATTGCCCATGCCCCCTGTGGAGGAGCTGGATGTCATGTTCAGTGAACTG GTGGATGAACTGGACCTCACAGACAAACACAGAGAAGCCATGTTTGCACTTCCAGCTGAG AAAAAATGGCAAATATACTGTAGCAAGAAAAAGGACCAGGAAGAAAACAAGGGAGCTACA AGTTGGCCTGAATTCTACATTGATCAGCTCAATTCCATGGCTGCTAGAAAATCTCTGCTG GCTTTAGAGAAGGAAGAAGAAGAAGAAAGAAGTAAAACTATAGAGAGTTTAAAGACAGCA CTGAGGACAAAACCAATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTATCATGT ATCCTCAACTTTCTAAAGACCATGGACTACGAGACCTCAGAGTCTCGAATACATACTTCT CTCATTGGCTGTATAAAGGCGTTAATGAACAACTCTCAAGGCCGGGCTCACGTCCTGGCT CATTCTGAGAGTATTAATGTAATTGCTCAGAGTCTGAGCACAGAGAACATTAAAACGAAG GTGGCCGTGCTGGAAATCTTGGGCGCCGTGTGCCTGGTTCCCGGGGGCCACAAGAAGGTT CTGCAGGCCATGCTGCACTACCAGAAGTATGCCAGCGAAAGGACCCGCTTTCAGACATTA ATTAACGACTTGGATAAAAGCACTGGGCGGTATCGAGATGAAGTGAGTCTCAAGACTGCC ATCATGTCCTTCATTAATGCAGTGCTCAGCCAAGGTGCAGGAGTGGAGAGTTTGGACTTT AGACTTCATCTTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTAATAGATAAATTA AGGGAACACGAAAATTCAACATTAGATAGGCATTTAGACTTTTTTGAAATGCTCCGAAAT GAAGATGAACTAGAATTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGTGCA ACTCAGATGTTTGAGCTGACCAGGAAGAGGCTGACACATAGTGAAGCTTACCCGCATTTC ATGTCCATCCTGCACCACTGCCTCCAAATGCCTTACAAGAGGAGTGGCAACACTGTTCAG TACTGGCTACTACTAGATAGAATTATACAGCAGATAGTTATCCAGAATGACAAAGGACAG GACCCTGACTCCACACCTTTGGAAAACTTTAATATTAAGAATGTCGTACGAATGTTGGTT AATGAAAATGAAGTTAAGCAGTGGAAAGAACAAGCGGAAAAAATGAGAAAAGAGCACAAT GAGCTACAGCAGAAACTGGAAAAGAAAGAACGAGAATGTGATGCTAAGACTCAAGAGAAG GAAGAGATGATGCAGACCTTAAATAAAATGAAAGAGAAACTTGAAAAGGAGACTACTGAG CATAAGCAAGTCAAGCAGCAGGTGGCGGACCTCACAGCACAGCTCCATGAGCTCAGCAGG AGGGCCGTCTGTGCTTCAATCCCAGGTGGACCCTCGCCTGGAGCACCAGGAGGGCCCTTT CCTTCCTCTGTGCCTGGATCTCTCCTTCCTCCCCCACCACCCCCACCTCTACCAGGTGGG ATGCTTCCCCCTCCACCGCCTCCCCTCCCTCCAGGTGGCCCTCCTCCTCCCCCAGGGCCT CCTCCCTTAGGGGCAATCATGCCACCTCCTGGTGCTCCAATGGGCCTAGCACTGAAGAAG AAAAGCATTCCTCAGCCCACAAATGCCCTGAAATCCTTCAACTGGTCTAAACTGCCCGAG AACAAACTGGAAGGAACAGTATGGACCGAAATTGATGATACAAAAGTCTTCAAAATTCTA GATCTTGAAGACCTGGAAAGAACCTTCTCTGCCTATCAAAGACAGCAGGATTTCTTTGTG AACAGTAACTCCAAGCAGAAAGAAGCAGATGCCATTGATGACACTCTGAGTTCCAAACTT AAAGTTAAAGAGCTTTCGGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTA TCGAGGTTGAAATTATCCAATGACGAAATCAAACGGGCAATTCTAACAATGGACGAACAG GAAGATCTGCCCAAGGACATGTTGGAACAGCTCTTGAAATTTGTTCCTGAAAAAAGTGAC ATTGACCTATTGGAGGAACATAAACACGAACTGGATCGGATGGCCAAGGCTGATAGGTTC CTTTTTGAGATGAGCCGAATTAATCACTATCAGCAAAGGTTGCAATCGCTGTACTTCAAA AAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAACCTAAAGTGGAAGCAATTCGTTCTGGC TCAGAAGAGGTGTTTAGGAGTGGTGCCCTCAAGCAGTTGCTGGAGGTGGTTTTGGCATTT GGAAATTATATGAATAAAGGTCAAAGAGGGAATGCATATGGATTCAAGATATCTAGCCTA AACAAAATTGCTGACACAAAATCCAGCATCGACAAAAACATTACCCTTTTGCACTATCTC ATCACTATTGTGGAAAATAAGTACCCCAGTGTTCTCAATCTAAATGAAGAATTGCGAGAT ATTCCTCAAGCTGCGAAAGTAAACATGACTGAGCTGGACAAAGAAATAAGTACCTTGAGA AGTGGCTTGAAAGCAGTAGAGACAGAGCTGGAATATCAGAAGTCTCAGCCCCCACAGCCC GGAGATAAGTTTGTGTCTGTTGTCAGCCAGTTCATCACAGTAGCCAGCTTCAGCTTCTCT GATGTTGAAGACCTTCTAGCAGAAGCTAAAGACCTGTTTACTAAAGCAGTGAAGCACTTT GGGGAAGAGGCTGGCAAAATACAACCAGATGAGTTCTTTGGCATTTTTGATCAATTTCTT CAAGCTGTGTCAGAAGCCAAACAAGAAAACGAAAATATGAGAAAGAAAAAGGAGGAAGAA GAACGTCGAGCTCGCATGGAAGCTCAGCTCAAAGAACAACGTGAAAGGGAACGTAAAATG AGAAAAGCTAAAGAGAATAGTGAAGAAAGCGGAGAGTTTGATGACCTTGTTTCAGCTTTA CGCTCAGGAGAAGTGTTTGACAAAGACCTTTCTAAATTGAAACGGAATCGCAAACGTATT ACCAACCAGATGACTGACAGCAGCAGAGAGAGACCAATCACAAAACTTAATTTCTAA Clone variation with respect to NM_014992.1 1389 a=>g >OriGene 5' read for NM_014992 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCCTACGAGCGGC TGTGAAGGAAACTGTTTAACCGGATCCCATTGTACCCAGAGTGCAGAGCCGCCTTTCCAG CATGCAGGGGCTGCTCAGCGTTTAGTCACATCAAGAAATAGAACAGAATTCAGCCATGGC CCCAAGAAAGAGAGGTGGACGAGGTATTTCATTCATCTTTTGCTGTTTCCGAAATAATGA TCACCCAGAAATCACGTATCGGCTGCGAAATGATAGCAACTTTGCGCTTCAGACCATGGA ACCAGCATTGCCCATGCCCCCTGTGGAGGAGCTGGATGTCATGTTCAGTGAACTGGTGGA TGAACTGGACCTCACAGACAAACACAGAGAAGCCATGTTTGCACTTCCAGCTGAGAAAAA ATGGCAAATATACTGTAGCAAGAAAAAGGACCAGGAAGAAAACAAGGGAGCTACAAGTTG GCCTGAATTCTACATTGATCAGCTCAATTCCATGGCTGCTAGAAAATCTCTGCTGGCTTT AGAGAAGGAAGAAGAAGAAGAAAGAAGTAAAACTATAGAGAGTTTAAAGACAGCACTGAG GACAAAACCAATGAGGGTTTGTAACCAGATTCATCGACTTGGATGGCCTATCATGTATCC TCAACTTTCTAAAGACCATGGACTACGAGACCTCAGAGTCTCGATACATACTTCTCTCAT TGGCTGTATAAAGGGCGTAATGAACAACTCTCAAGGNCGGGCTCACGTNCTGGCTCATTC TGAGAGTATTAATGGTATTTGCTCAAAGTCTGAGCACAGAGAACATTANNAACGANGGTT GGCCGTGCTTGGAAATCTTNGGCCGCCGTGTTGCTGGGTTNCCGGGGGCACAAANAAAGT TCTCGCAGNCCATGCTGCACTACCAAAGTATG >OriGene 3' read for NM_014992 unedited
TAGCTTTGAACCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGTAATC TTTATCCCTTCAAAAAATCTTCCTTTAAGTCTTGGGTGAGATATTTTATTATTTTTAAGG TTTATATATCTATATTGTTAGAACAAGCATACTCTGGCAATGTTGTTCTTTAAAAAAAGC ATGGATTGCATTTATATAGTGTTTTTTTCCAAAAATCTGAAGAAATTATCAAGAGCACTT GGCAGAGATAACTTGATGAAATCAGGGGAAAAAATTGATGTCCAACTTTTAAATATACAA AAACTTGATTGACATTCTACATAATTCAAATTGTGTGCAAGATGTTCTCAATAAACATTT ACAGACTGTATCCCCACACCAATGTCAGAAATTCAAAGCTGGTCCTATTACTGATGTGAT ACATCAACATCACAATGTCAGCTATCAAAAATCATTTTAAATCTATGTTTTTCCAACAAC TCCAGTTCCATCAATGAAAATAATTTTAAAGGATATCAAGCTTGGAAACCATAAAATATT TTGCAAGCTTTTTTTGTTTGTTTTGTTTGTTTCTTCTGTTTTGCCTTTGAATCAGGGAAA TGTCTCTTATCTCTTTCAGGAGATAGTATTGTGCTTCTGAACAGTATGGCGTTCGGNGAA AGAAAATGAGCCCACTCAATGTGAATGCATACACGAAAAGTTTTGNAGCACATACATTAA CATAANATCTTTTAACCATACGTTAATAGGTCTCCAGACAACACTCAGACATCTATATAC AATTTTTTAATATATACACACATACATTTACATACACACGTTCACACATTCACTCACAGA TGAAGAANAAATGCCACTNTTTGAATGCCAAGNANTGTAATGAAAACTTCTAGTACTTTT AAAGGCTGCTATGAGCTCTAAAAAAGAATCATGAAATNAAATAAATTTNGGAATGGNCCT TCTGCTCTTGAAAATTGGGGGGAAACTAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_014992 |
Insert Size | 4600 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014992.1, NP_055807.1 |
RefSeq Size | 4256 bp |
RefSeq ORF | 3237 bp |
Locus ID | 23002 |
UniProt ID | Q9Y4D1 |
Domains | FH2 |
Protein Pathways | Wnt signaling pathway |
Gene Summary | Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217675 | DAAM1 (Myc-DDK-tagged)-Human dishevelled associated activator of morphogenesis 1 (DAAM1) |
CNY 7,504.00 |
|
RC217675L1 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), Myc-DDK-tagged |
CNY 9,904.00 |
|
RC217675L2 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), mGFP tagged |
CNY 12,640.00 |
|
RC217675L3 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), Myc-DDK-tagged |
CNY 12,640.00 |
|
RC217675L4 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), mGFP tagged |
CNY 12,640.00 |
|
RG217675 | DAAM1 (tGFP-tagged) - Human dishevelled associated activator of morphogenesis 1 (DAAM1) |
CNY 9,104.00 |