ALKBH3 (NM_139178) Human Untagged Clone

CAT#: SC120570

ALKBH3 (untagged)-Human alkB, alkylation repair homolog 3 (E. coli) (ALKBH3)



  "NM_139178" in other vectors (6)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit anti-ALKBH3 Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ABH3; DEPC-1; DEPC1; hABH3; PCA1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_139178 edited
ATGGAGGAAAAAAGACGGCGAGCCCGAGTTCAGGGAGCCTGGGCTGCCCCTGTTAAAAGC
CAGGCCATTGCTCAGCCAGCTACCACTGCTAAGAGCCATCTCCACCAGAAGCCTGGCCAG
ACCTGGAAGAACAAAGAGCATCATCTCTCTGACAGAGAGTTTGTGTTCAAAGAACCTCAG
CAGGTAGTACGTAGAGCTCCTGAGCCACGAGTGATTGACAGAGAGGGTGTGTATGAAATC
AGCCTGTCACCCACAGGTGTATCTAGGGTCTGTTTGTATCCTGGCTTTGTTGACGTGAAA
GAAGCTGACTGGATATTGGAACAGCTTTGTCAAGATGTTCCCTGGAAACAGAGGACCGGC
ATCAAAGAGGATATAACTTATCAGCAACCAAGACTTACAGCATGGTATGGAGAACTTCCT
TACACTTATTCAAGAATCACTATGGAACCAAATCCTCACTGGCACCCTGTGCTGCGCACA
CTAAAGAACCGCATTGAAGAGAACACTGGCCACACCTTCAACTCCTTACTCTGCAATCTT
TATCGCAATGAGAAGGACAGCGTGGACTGGCACAGTGATGATGAACCCTCACTAGGGAGG
TGCCCCATTATTGCTTCACTAAGTTTTGGTGCCACACGCACATTTGAGATGAGAAAGAAG
CCACCACCAGAAGAGAATGGAGAGTACACATATGTGGAAAGAGTGAAGATACCCTTGGAT
CATGGGACCTTGTTAATCATGGAAGGAGCGACACAAGCTGACTGGCAGCATCGAGTGCCC
AAAGAATACCACTCTAGAGAACCGAGAGTGAACCTGACCTTTCGGACAGTCTATCCAGAC
CCTCGAGGGGCACCCTGGTGA
>OriGene 5' read for NM_139178 unedited
GGGGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCGCGCC
CGGCTCCGCCCGCAAGTGCGCCTTCCTGACTTACTGCTGGGTGCGCGGGGCTGGGGGTGC
GAGTACCACCCCTGAAGTCTCTTCCTGGGCGACCTCCGGGGCCTCATTCTAGGCCTCCTT
AAAGAGAAGGATCTAAATTAGGAAAAGGAAGTGCCCTTATCCACGACCAAGCTCTTCCAC
CTGCGGAGCTCGCTTAGTCTGCACCTCAACCGTGCGGAAAGTGACTGCCCTGTTTACTGA
GGAAAAACTGGGGCTCAGAAAGATACCATGGAGTAGTTTGAAACAGGAACAAAATCTTCT
GAAAGCTCGGAGCAGAAGCCTTTTTGGTCAACATGGAGGAAAAAAGACGGCGAGCCCGAG
TTCAGGGAGCCTGGGCTGCCCCTGTTAAAAGCCAGGCCATTGCTCAGCCAGCTACCACTG
CTAAGAGCCATCTCCACCAGAAGCCTGGCCAGACCTGGAAGAACAAAGAGCATCATCTCT
CTGACAGAGAGTTTGTGTTCAAAGAACCTCAGCAGGTAGTACGTAGAGCTCCTGAGCCAC
GAGTGATTGACAGAGAGGGTGTGTATGAAATCAGCCTGTCACCCACAGGTGTATCTAGGG
TCTGTTTGTATCCTGGCTTTGTTGACGTGAAAGAAGCTGACTGGATATTGGAACAGCTTT
GTCAAGATGTTCCCTGGAAACAGAGGACCGGCATCAAAGAGGATATAACTTATCAGCAAC
CAAGACTTACAGCATGGTATGGAGAACTTCCTTACACTTATTCAAGAATCACTATGGAAC
CAAATNCTCACTGGCACCCTGTGCTGCGCACACTAAAGAACCGCATTTGAGAGAACACTG
GCCACACTTNCACTCCTTACTCTGCAATCTT
>OriGene 3' read for NM_139178 unedited
NNGGCCTTGACCTGCCCATANATTTAATTGCCTAAGNAGATAGCCATTTTGAGCTGTGGT
TAGGGATAACTGTTCCCACAGTAGACCTATTACCAACATGAGTTGCTGGCTTTCATTTAA
TAGGACTCCAAGCTGGCAAACAAACCCCACCATCTTCCCAACAGCCACATCATGAGATCT
AGCAGCACCAGCCTCTTGAAGTGGCTTCTCAGTGGAAGGTTTGCTCCGTTTCAGTGAAGC
TTCTCTCTCAAAGCTCTGACGTCACCAGGGTGCCCCTCGAGGGTCTGGATAGACTGTCCG
AAAGGTCAGGTTCACTCTCGGTTCTCTAGAGTGGTATTCTTTGGGCACTCGATGCTGCCA
GTCAGCTTGTGTCGCTCCTTCCATGATTAACAAGGTCCCATGATCCAAGGGTATCTTCAC
TCTTTCCACATATGTGTACTCTCCATTCTCTTCTGGTGGTGGCTTCTTTCTCATCTCAAA
TGTGCGTGTGGCACCAAAACTTAGTGAAGCAATAATGGGGCACCTCCCTAGTGAGGGTTC
ATCATCACTGTGCCAGTCCACGCTGTCCTTCTCATTGCGATAAAGATTGCAGAGTAAGGA
GTTGAAGGTGTGGCCAGTGTTCTCTTCAATGCGGTTCTTTAGTGTGCGCAGCACAGGGTG
CCAGTGAGGATTTGGTTCCATAGTGATTCTTGAATAAGTGTAAGGAAGTTCTCCATACCA
TGCTGTAAGTCTTGGTTGCTGATAAGTTATATCCTCTTTGATGCCGGTCCTCTGTTTCCA
GGGAACATCTTGACAAAGCTGTTCCCATATCCAGTCAGCTTCTTTCACGTCAACAAAGCC
AGGATACAAACAGACCCTAGATACACCTGTGGGGTGACAGGCTGATTTCATAA
Restriction Sites NotI-NotI     
ACCN NM_139178
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_139178.1, NP_631917.1
RefSeq Size 1520 bp
RefSeq ORF 861 bp
Locus ID 221120
UniProt ID Q96Q83
Domains 2OG-FeII_Oxy
Protein Families Druggable Genome
Gene Summary The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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