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KCNN4 (NM_002250) Human Untagged Clone
CAT＃: SC121952
KCNN4 (untagged)-Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4)
CNY 5,488.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCA1; KCa3.1; KCA4; SK4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC121952 sequence for NM_002250 edited (data generated by NextGen Sequencing)
ATGGGCGGGGATCTGGTGCTTGGCCTGGGGGCCTTGAGACGCCGAAAGCGCTTGCTGGAG CAGGAGAAGTCTCTGGCCGGCTGGGCACTGGTGCTGGCAGGAACTGGCATTGGACTCATG GTGCTGCATGCAGAGATGCTGTGGTTCGGGGGGTGCTCGTGGGCGCTCTACCTGTTCCTG GTTAAATGCACGATCAGCATTTCCACCTTCTTACTCCTCTGCCTCATCGTGGCCTTTCAT GCCAAAGAGGTCCAGCTGTTCATGACCGACAACGGGCTGCGGGACTGGCGCGTGGCGCTG ACCGGGCGGCAGGCGGCGCAGATCGTGCTGGAGCTGGTGGTGTGTGGGCTGCACCCGGCG CCCGTGCGGGGCCCGCCGTGCGTGCAGGATTTAGGGGCGCCGCTGACCTCCCCGCAGCCC TGGCCGGGATTCCTGGGCCAAGGGGAAGCGCTGCTGTCCCTGGCCATGCTGCTGCGTCTC TACCTGGTGCCCCGCGCCGTGCTCCTGCGCAGCGGCGTCCTGCTCAACGCTTCCTACCGC AGCATCGGCGCTCTCAATCAAGTCCGCTTCCGCCACTGGTTCGTGGCCAAGCTTTACATG AACACGCACCCTGGCCGCCTGCTGCTCGGCCTCACGCTTGGCCTCTGGCTGACCACCGCC TGGGTGCTGTCCGTGGCCGAGAGGCAGGCTGTTAATGCCACTGGGCACCTTTCAGACACA CTTTGGCTGATCCCCATCACATTCCTGACCATCGGCTATGGTGACGTGGTGCCGGGCACC ATGTGGGGCAAGATCGTCTGCCTGTGCACTGGAGTCATGGGTGTCTGCTGCACAGCCCTG CTGGTGGCCGTGGTGGCCCGGAAGCTGGAGTTTAACAAGGCAGAGAAGCACGTGCACAAC TTCATGATGGATATCCAGTATACCAAAGAGATGAAGGAGTCCGCTGCCCGAGTGCTACAA GAAGCCTGGATGTTCTACAAACATACTCGCAGGAAGGAGTCTCATGCTGCCCGCAGGCAT CAGCGCAAGCTGCTGGCCGCCATCAACGCGTTCCGCCAGGTGCGGCTGAAACACCGGAAG CTCCGGGAACAAGTGAACTCCATGGTGGACATCTCCAAGATGCACATGATCCTGTATGAC CTGCAGCAGAATCTGAGCAGCTCACACCGGGCCCTGGAGAAACAGATTGACACGCTGGCG GGGAAGCTGGATGCCCTGACTGAGCTGCTTAGCACTGCCCTGGGGCCGAGGCAGCTTCCA GAACCCAGCCAGCAGTCCAAGTAG Clone variation with respect to NM_002250.2 >OriGene 5' read for NM_002250 unedited
GGCAAAGCGCNAAAAANACNNNACTCTCCCCCCNCNNCGGTTCAGAATTTGTNATACGAC TCACTATAGGGCGGCCGCGAATTCGCACCAGGGCAGGAAGCTGGCTGAGCCCCAAAACCC CGGGGGCCATGGGCGGGGATCTGGTGCTTGGCCTGGGGGCCTTGAGACGCCGAAAGCGCT TGCTGGAGCAGGAGAAGTCTCTGGCCGGCTGGGCACTGGTGCTGGCAGGAACTGGCATTG GACTCATGGTGCTGCATGCAGAGATGCTGTGGTTCGGGGGGTGCTCGTGGGCGCTCTACC TGTTCCTGGTTAAATGCACGATCAGCATTTCCACCTTCTTACTCCTCTGCCTCATCGTGG CCTTTCATGCCAAAGAGGTCCAGCTGTTCATGACCGACAACGGGCTGCGGGACTGGCGCG TGGCGCTGACCGGGCGGCAGGCGGCGCAAATCGTGCTGGAGCTGGTGGTGTGTGGGCTGC ACCCGGCGCCCGTGCGGGGCCCGCCGTGCGTGCAGGATTTAGGGGCGCCGCTGACCTCCC CGCAGCCCTGGCCGGGATTCCTGGGCCAAGGGGAAGCGCTGCTGTCCCTGGCCATGCTGC TGCGTCTCTACCTGGTGCCCCGCGCCGTGCTCCTGCGCAGCGGCGTCCTGCTCAACGCTT NCTACCGCAGCATCGGCGCTCTCAATCAAGTCCGCTTCCGCCACTGGTTCGTGGCCCAGC TTTACATGAACACGCNACCCTGGCCGNCTGCTGNCTCGCCTCACGCTTGGCCTCTGGCTG ACCACCGNCTGNGTGCTGTCCGNTGGCGANAAGCTGTTAATGCCACTGGGCACCTTTAGA CCACTTTGGCTGACCCCATACATTCCTGACATCGNTATGGGGAACTGTGCCGGCCACATG TGGGGCCAGATCTCTGGCTGGCACTGGAGCATGGTGTCTGCTGACAGCCCTGCTGTGGCC GGGTGGCCCN >OriGene 3' read for NM_002250 unedited
NCCGTTCTACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTT TTTTTGGCTTTAACATTTATTACAAAGAAAAGAGCAGAGGCTGGTGAGTTACACTTCTTC CTCCCCACCAGGTGCTCTCTGCAGCTCTGGAAAAATGGTGTCCTCTTTGTTGTCCCACCA GGGGGCGCCACCTCCAGCCCCGCCCCAGCCTCATACCCAGTTCTTCAGCTCGGCCAGCGG TAACTGAAGCCTCCCAGAATCCTGGATCCGGGCCCCTAGTACCCTCTTTCCCAGGGACCC AGGAGTCCTGCCTCCAGTCGCCTGCACTTGTAACTGAGAGCTGGAGGTCGTCCATAGCAG CATAGTGAGAGTGTTTTTGATGAGGGTATGCAGAGTGGGGGTGACCATGTTCCCACCTGG GGCCTCAGGTGGGCCAAGGCCTACCCACTTTAGCCAGCGTCCCCTCCAGCAGCCATCAGC AAGCCAACCCACTCCAAGCCAGGGCCCCCTTTGGTCCTTGCACTTGAGGTGCTTTGTTCA GGGCTGGGTCAGGAGTGGCAGAGACGATGTCCACCACCTCAGTACTGGGGAAAGTAGCCT GGTTCCTCCTCGTGGGTCCAGCTACTTGGACTGCTGGCTGGGTTCTGGAAGCTGCCTCGG CCCCAGGGCAGTGCTAAGCAGCTCAGTCAGGGCATCCAGCTTCCCCGCCAGCGTGTCAAT CTGTTTCTCCAGGGCCCGGTGTGAGCTGCTCANATTCTGCTGCAGGTCATACAGGATCAT GTGCATCTTGGAGATGTCCACCATGGAGTTCACTTGTTCCCGGAGCTCTCGTGTTTCAAC CGCACCCTGCGGACCCGTTGTGGCGGCCAGCAACTTGCGCTGATGCCTGCCGGCAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002250 |
| Insert Size | 2050 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002250.2, NP_002241.1 |
| RefSeq Size | 2240 bp |
| RefSeq ORF | 1284 bp |
| Locus ID | 3783 |
| UniProt ID | O15554 |
| Domains | SK_channel, CaMBD |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Gene Summary | The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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KCa3.1/IK1 Channel Regulation by cGMP-Dependent Protein Kinase (PKG) via Reactive Oxygen Species and CaMKII in Microglia: An Immune Modulating Feedback System?
,Ferreira, R;Wong, R;Schlichter, LC;,
Front Immunol
,PubMed ID 25904916
[KCNN4]
|
|
KCa3.1/IK1 channel regulation by cGMP-dependent protein kinase (PKG)via reactive oxygen species and CaMKII in microglia: An immunemodulating feedback system?
,Ferreira, R;Wong, R;Schlichter, L;,
Name: Frontiers in Immunology
[KCNN4]
|
|
PKA Reduces the Rat and Human KCa3.1 Current, CaM Binding, and Ca2+ Signaling, Which Requires Ser332/334 in the CaM-Binding C Terminus
,Wong, R;Schlichter, LC;,
J. Neurosci.
,PubMed ID 25274816
[KCNN4]
|
|
IK1 channel activity contributes to cisplatin sensitivity of human epidermoid cancer cells
,Elbert L. Lee, Yuichi Hasegawa, Takahiro Shimizu, and Yasunobu Okada,
Am J Physiol Cell Physiol, Jun 2008; 294: C1398 - C1406.
[KCNN4]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204457 | KCNN4 (Myc-DDK-tagged)-Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4) |
CNY 5,488.00 |
|
| RC204457L1 | Lenti ORF clone of Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC204457L2 | Lenti ORF clone of Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4), mGFP tagged |
CNY 5,890.00 |
|
| RC204457L3 | Lenti ORF clone of Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204457L4 | Lenti ORF clone of Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4), mGFP tagged |
CNY 7,888.00 |
|
| RG204457 | KCNN4 (tGFP-tagged) - Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4) |
CNY 7,088.00 |
|
| SC322489 | KCNN4 (untagged)-Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4) |
CNY 5,488.00 |
