CLCNKB (BC020873) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Chloride channel, kidney, B; chloride channel Kb; ClC-K2; CLCKB; hClC-Kb; MGC24087; OTTHUMP00000011120
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC020873 edited GGGGGGAGGATGTTGATTGTTGGAACACACACCTGTCCAGGTTCAGGGGAGCTGGAGGCT CTGTGAGAGGAGGGCCAGCTCAGCCACAGCAGGAGGACTGACAGGGGCCTGATGGAGGAG TTTGTGGGGCTGCGTGAAGGCTCCTCAGGGAACCCTGTGACTCTGCAGGAGCTGTGGGGC CCCTGTCCCCGCATCCGCCGAGGCATCCGAGGTGGCCTGGAGTGGCTGAAGCAGAAGCTC TTCCGCCTGGGCGAGGACTGGTACTTCCTGATGACCCTCGGGGTGCTCATGGCCCTGGTC AGCTGTGCCATGGACTTGGCTGTTGAGAGTGTGGTCCGAGCGCACCAGTGGCTGTACAGG GAGATTGGGGACAGCCACCTGCTCCGGTATCTCTCCTGGACTGTGTACCCTGTGGCCCTC GTCTCTTTCTCTTCGGGCTTCTCTCAGAGCATCACACCCTCCTCTGGAGGTTCTGGAATC CCGGAGGTGAAGACCATGTTGGCGGGTGTGGTCTTGGAGGACTACCTGGATATCAAGAAC TTTGGGGCCAAAGTGGTGGGCCTCTCCTGCACCCTGGCCTGTGGCAGCACCCTCTTCCTC GGCAAAGTGGGCCCTTTCGTGCACCTGTCTGTGATGATGGCTGCCTACCTGGGCCGTGTG CGCACCACGACCATCGGGGAGCCTGAGAACAAGAGCAAGCCTGTGTACTCCGCTCTGGCC ACCTTGGTTCTCGCCTCCATCACCTACCCACCCAGCGCCGGCCGCTTCCTAGCTTCTCGG CTGTCCATGAAGCAGCATCTGGACTCGCTGTTCGACAACCACTCCTGGGCGCTGATGACC CAGAACTCCAGCCCACCCTGGCCCGAGGAGCTCGACCCCCAGCACCTGTGGTGGGAATGG TACCACCCGCGGTTCACCATCTTTGGGACCCTTGCCTTCTTCCTGGTTATGAAGTTCTGG ATGCTGATTCTGGCCACCACCATCCCCATGCCTGCCGGGTACTTCATGCCCATCTTTGTC TATGGAGCTGCTATCGGGCGCCTCTTTGGGGAGACTCTCTCTTTTATCTTCCCTGAGGGC ATCGTGGCTGGAGGGATCACCAATCCCATCATGCCAGGGGGGTATGCTCTGGCAGGGGCT GCAGCCTTCTCAGGGGCTGTGACCCACACCATCTCCACGGCGCTGCTGGCCTTCGAGGTG ACCGGCCAGATAGTGCATGCACTGCCCGTGCTGATGGCGGTGCTGGCAGCCAACGCCATT GCACAGAGCTGCCAGCCCTCCTTCTATGATGGCACCGTCATTGTCAAGAAGCTGCCATAC CTGCCACGGATTCTGGGCCGCAACATCGGTTCCCACCGCGTGAGGGTGGAGCACTTCATG AACCACAGCATCACCACACTGGCCATCACATTAATGAATGATGAGATTGGAGTACACTGT CACCAAGGGCAGGCACAGATGGCTTCTGGGGTTGTCTGGTTCCCAGTGAGAGGCTCCTGA GAAAAATAAAGCTGGTTCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020873 unedited NNNCCACTTTGTTCGCATTTGTGTACGACTCATATAGGCGGCCGCGTAATTCGCCATTAC GGCCGGGGGGGGGGAGGATGTTGATTGTTGGAACACACACCTGTCCAGGTTCAGGGGAGC TGGAGGCTCTGTGAGAGGAGGGCCAGCTCAGCCACAGCAGGAGGACTGACAGGGGCCTGA TGGAGGAGTTTGTGGGGCTGCGTGAAGGCTCCTCAGGGAACCCTGTGACTCTGCAGGAGC TGTGGGGCCCCTGTCCCCGCATCCGCCGAGGCATCCGAGGTGGCCTGGAGTGGCTGAAGC AGAAGCTCTTCCGCCTGGGCGAGGACTGGTACTTCCTGATGACCCTCGGGGTGCTCATGG CCCTGGTCAGCTGTGCCATGGACTTGGCTGTTGAGAGTGTGGTCCGAGCGCACCAGTGGC TGTACAGGGAGATTGGGGACAGCCACCTGCTCCGGTATCTCTCCTGGACTGTGTACCCTG TGGCCCTCGTCTCTTTCTCTTCGGGCTTCTCTCAGAGCATCACACCCTCCTCTGGAGGTT CTGGAATCCCGGAGGTGAAGACCATGTTGGCGGGTGTGGTCTTGGAGGACTACCTGGATA TCAAGAACTTTGGGGCCAAAGTGGTGGGCCTCTCCTGCACCCTGGCCTGTGGCAGCACCC TCTTCCTCGGCAAAGTGGGCCCTTTCGTGCACCTGTCTGTGATGATGGCTGCCTACCTGG GCCGTGTGCGCACCACGACCCATCGGGAGCCTGAGAACAAGAGCAAGCCTGTGTACTCCG CTCTGGCCCCTTGGTTCTCGCCTTCATCACCTACCCACCCAGCGCCGGCCGCTTCCTAGC TTCTCGGCTGTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC020873 unedited GCATGTCACTTCCACGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGGGCCGAGGCGGCCG ACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGGGAACCAGCTTTATTTTTCTCA GGAGCCTCTCACTGGGAACCAGACAACCCCAGAAGCCATCTGTGCCTGCCCTTGGTGACA GTGTACTCCAATCTCATCATTCATTAATGTGATGGCCAGTGTGGTGATGCTGTGGTTCAT GAAGTGCTCCACCCTCACGCGGTGGGAACCGATGTTGCGGCCCAGAATCCGTGGCAGGTA TGGCAGCTTCTTGAACAATGACGGTGCCATCATAGAAAGAGGGCTGGCAGCTCTGTGCAA TGGCGTTGGGCTGCCAGCCACNCGCCATCAGCACGGGCCAGTGCATTGCACTTATCTTGC CCGGGTCACCCTCGAAAGGGCCANCAANCGCCCGTTGGNAAAATGGGTGGTGGGGGTTCA CCAAGGCCCCCCTGTAAGAAAGGGGCTTGGCAAGCCCCCCCTTGCCCCAGGAGAGCATTT ACCCCCCCCCCTTGGGCCATTGTGATGGGGGGAATTTGGGGGTGGATTCCCCCTTTCCCA AGCCCCACCGGAATTGGCCCCTCCAGGGGGAGAGGAATATAAAAGGAAGAGGAGTTCTTC CCCCAAAAAGAAGGCCGCCCCGGATTAGCAAGCTTCCATAAGACAAAAGATGGGGCATGA AAGTACCCCGGCAGGCATGGGNGATGGTGGTTGCCAAGAATCAGCATCCAGAACTTCTAA CCCGGAAGAAGGCAAGGTCCCA
Restriction Sites	Please inquire
ACCN	BC020873
Insert Size	1552 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC020873.1, AAH20873.1
RefSeq Size	1552 bp
RefSeq ORF	1386 bp
Locus ID	1188
Protein Families	Druggable Genome, Transmembrane
Gene Summary	The protein encoded by this gene is a member of the family of voltage-gated chloride channels. Chloride channels have several functions, including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. This gene is expressed predominantly in the kidney and may be important for renal salt reabsorption. Mutations in this gene are associated with autosomal recessive Bartter syndrome type 3 (BS3). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

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