PLA2G1B (BC005386) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MGC119834; MGC119835; phospholipase A2, group IB; phospholipase A2, group IB (pancreas); PLA2; PLA2A; PPLA2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC005386 edited AGTTCTTTTCTCACCTTGACTGCAAGATGAAACTCCTTGTGCTAGCTGTGCTGCTCACAG TGGCCGCCGCCGACAGCGGCATCAGCCCTCGGGCCGTGTGGCAGTTCCGCAAAATGATCA AGTGCGTGATCCCGGGGAGTGACCCCTTCTTGGAATACAACAACTACGGCTGCTACTGTG GCTTGGGGGGCTCAGGCACCCCCGTGGATGAACTGGACAAGCAAAAACAAAGAGTGTGAG GCCTTCATTTGCAACTGCGACCGCAACGCTGCCATCTGCTTTTCAAAAGCTCCATATAAC AAGGCACACAAGAACCTGGACACCAAGAAGTATTGTCAGAGTTGAATATCACCTCTCAAA AGCATCACCTCTATCTGCCTCATCTCACACTGTACTCTCCAATAAAGCACCTTGTTGAAA GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC005386 unedited NNGGTTTGAGTCAAATTTGTATACGACTCATATAGGCGGCACGCGAATCGGCCATTACGG CCGGGGAGTTCTTTTCTCACCTTGACTGCAAAATGAAACTCCTTGTGCTAGCTGTGCTGC TCACAGTGGCCGCCGCCGACAGCGGCATCAGCCCTCGGGCCGTGTGGCAGTTCCGCAAAA TGATCAAGTGCGTGATCCCGGGGAGTGACCCCTTCTTGGAATACAACAACTACGGCTGCT ACTGTGGCTTGGGGGGCTCATGCACCCCCGTGGATGAACTGGACAAGCAAAAACAAAGAG TGTGAGGCCTTCATTTGCAACTGCGACCGCAACGCTGCCATCTGCTTTTCAAAAGCTCCA TATAACAAGGCACACAAGAACCTGGACACCAAGAAGTATTGTCAGAGTTGAATATCACCT CTCAAAAGCATCACCTCTATCTGCCTCATCTCACACTGTACTCTCCAATAAAGCACCTTG TTGAAAGACAAANNANAAAAAAAAAAAAAAAAAAAAAAACATGTCGGCCGCCTCGGCCCT CGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCC TGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGC CTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTA TGGGGTGGAGGGGGNTGGGTATTGGAGCAAGGGGCAAGTTGGGAAAGACACCTGTAGGGC CTGCGGGGTCTATTGGGAACCAGCTGGATGCAGTGCNCAATCTTGGCTACTGCAATCTCG CCTCCTGGGTTCAGCGATT
Restriction Sites	Please inquire
ACCN	BC005386
Insert Size	453 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005386.1, AAH05386.1
RefSeq Size	453 bp
Locus ID	5319
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
Gene Summary	This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

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