MTRF1 (BC027731) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MGC47721; mitochondrial translational release factor 1; mitochontrial peptide chain release factor 1; MRF1; MTTRF1; OTTHUMP00000018315; OTTHUMP00000018316; RF1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC027731 edited GGCACGAGGCGGGTTTGTCGGGCTGAAATGTGGCGGGTCTCGGAAGGTTCCGACCTCAGT AAAGAGAGCTAACGTGTATTCTTCTTTTTCTTAGCGTGTTGCTTTGTCGCCCACGCTGGT TGCAGTGGTGCGATCTCAGTTCACTGCAACCTTCGCCTCCCAGGTTCAAGCGATTATCCT GCCTCAGCTTCCCGAGCAGCTAGGACTACAGGTGTGTGCCATCACGCTCAGCTAATTTTT TTGGTATTTTTAGTAGAGATGGGGTTTTCCCATGTTGGCCAGGCTGGTCTCGAACTCCTG ACCTCAGATGATCTGCCCGCCTCGGCCTCCCAAAGTGGTAGGATTACAGGCGTGAGCCAC TGTGCTCGGCACATTTTGATGCTGAGATGAATCGTCACCTGTGTGTTTGGCTTTTTAGAC ATCCATCTCTTAATGGTTACCTCCAGTGTCACATCCAGCTCCATTCTCATCAATTTAGAC AGATACATCTTGATACAAGGCTGCAAGTTTTTAGACAAAACAGGAATTGCATTCTTCATC TGTTAAGTAAGAATTGGTCCAGGAGATATTGCCATCAAGACACCAAGATGCTCTGGAAGC ATAAAGCACTACAGAAATATATGGAGAACCTGAGTAAGGAGTACCAAACACTTGAGCAAT GTCTGCAGCATATCCCTGTGAATGAGGAAAACCGAAGGTCCTTGAACAGAAGGCATGCTG AGTTGGCACCTCTTGCAGCCATTTACCAAGAAATTCAGGAGACTGAACAAGCAATTGAAG AATTAGAATCAATGTGTAAAAAGACAGAGTCTTGCTCTGTGGCCCAGGCTGGAATGCAGT AGTGTAATCATAGCTCACTTTAACCTTGAACTCCTGGACTCAAGCAATCCTCCAACCTCA GCCTCCCAAGTAGCTGGGACTACAGGCCTAAATAAACAAGATGAAAAGCAGTTACAAGAA CTTGCACTGGAAGAAAGGCAAACCATTGATCAAAAAATCAACATGTTGTACAATGAGCTT TTCCAGAGCCTTGTGCCAAAGGAGAAATATGACAAAAATGATGTTATTTTAGAGGTGACA GCTGGAAGGACTACTGGAGGTGACATCTGCCAACAATTTACCCGAGAAATATTTGACATG TACCAGAATTATTCGTGCTATAAACACTGGCAATTTGAACTTCTGAATTATACACCAGCA GATTATGGTAGGCATATTGAGGAATTTGCCTGTAGGAGATACTGAAAATTATTTTACCTC TGATCTTGATTAGAACCATATTGTCCCATTTTTTTTTGAAACTTTTGGTGTATATCTACT AAACACTGTGGCATAGGCTCATCAATCACAGTGGCTTACTATTATAGCAGAGTTGTTAGC TAGGAGAAGACAGTAGAAAGGAGGGAAGCATTTGCTTAGGGAGAAATATGTGAATTAGAG AAAATTCCCCTGAGGAACTTAGATACGTATCTTGCCTCAAAATCATTTTTCTCTATTATT GAAAGCAAAGGTATATTAAGGTTTTTACTTTAAATAACTTTATAAAATAATATAAGTATT ACTAATTAGAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC027731 unedited GGGNNCGGCCGCGCAATTCGGCACGAGGCGGGTTTGTCGGGCTGAAAGTGGCGGGTCTCG GAAGGTTCCGACCTCAGTAAAGAGAGCTAACGTGTATTCTTCTTTTTCTTAGCGTGTTGC TTTGTCGCCCACGCTGGTTGCAGTGGTGCGATCTCAGTTCACTGCAACCTTCGCCTCCCA GGTTCAAGCGATTATCCTGCCTCAGCTTCCCGAGCAGCTAGGACTACAGGTGTGTGCCAT CACGCTCAGCTAATTTTTTTGGTATTTTTAGTAGAGATGGGGTTTTCCCATGTTGGCCAG GCTGGTCTCGAACTCCTGACCTCAGATGATCTGCCCGCCTCGGCCTCCCAAAGTGGTAGG ATTACAGGCGTGAGCCACTGTGCTCGGCACATTTTGATGCTGAGATGAATCGTCACCTGT GTGTTTGGCTTTTTAGACATCCATCTCTTAATGGTTACCTCCAGTGTCACATCCAGCTCC ATTCTCATCAATTTAGACAGATACATCTTGATACAAGGCTGCAAGTTTTTAGACAAAACA GGAATTGCATTCTTCATCTGTTAAGTAAGAATTGGTCCAGGAGATATTGCCATCAAGACA CCAAGATGCTCTGGAAGCATAAAGCACTACAGAAATATATGGAGAACCTGAGTAAGGAGT ACCAAACACTTGAGCAATGTCTGCAGCATATCCCTGTGAATGAGGGAAAACCGAAGTCCT TGAACAGAANGCATGCTGAGTTGGCACCTCTTGCAGCCATTTACCAAGAAATTCANGAGA CTGNNACAGCATTGNAGAATTAGNNATCAATGTGTAAAAGACAGAGTCTTGCTCTGTGC
Restriction Sites	Please inquire
ACCN	BC027731
Insert Size	1595 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027731.1, AAH27731.1
RefSeq Size	1595 bp
Locus ID	9617
Gene Summary	The protein encoded by this gene was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear. [provided by RefSeq, Jul 2008]

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