KCC4 (SLC12A7) (BC007760) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp434F076; KCC4; potassium/chloride transporter KCC4; solute carrier family 12 (potassium/chloride transporters), member 7
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC007760 edited GGCACGAGGGCAGCGCGGGCCGGGCCGGGACGGGGACTGTCGGCTGCAGGCGGCCATGCC CACCAACTTCACCGTGGTGCCCGTGGAGGCTCACGCCGACGGCGGCGGGGACGAGACTGC CGAGCGGACGGAGGCTCCGGGCACCCCCGAGGGCCCCGAGCCCGAGCGCCCCAGCCCGGG AGATGGAAATCCAAGAGAAAACAGCCCATTCCTCAACAATGTCGAGGTGGAACAAGAGAG CTTCTTTGAAGGGAAGAACATGGCACTTTTCGAGGAGGAGATGGACAGTAACCCCATGGT GTCCTCGCTGCTCAACAAGCTGGCCAACTACACCAACCTGAGCCAGGGCGTGGTGGAGCA CGAGGAGGACGAGGAGAGCCGGCGGCGGGAGGCCAAGGCTCCGCGCATGGGCACCTTCAT CGGCGTCTACCTGCCGTGCCTGCAGAACATCCTGGGCGTCATCCTCTTCCTGCGCCTGAC GTGGATCGTGGGGGTGGCTGGTGTCCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCAC ATGTACAATGCTGACCGCCATTTCCATGAGTGCGATCGCTACCAACGGTGTGGTCCCAGC TGGCGGGTCCTACTACATGATATCGCGCTCGCTGGGACCCGAGTTTGGAGGCGCTGTCGG CCTCTGCTTCTACCTGGGCACGACGTTTGCAGGGGCCATGTATATTTTGGGGACCATCGA GATTTTTCTGACAGGCACGGAGGACGGCGTGGGCAGCCTGGGCCTGGGCTTGGCCCAGGG AAACAGACGGCAGACACACCTGTCCCCCAGTGATGCCGCCCAAGCTGCCCATGGGGCTTC CTACGGAAGTTTCTAGGCCCGTCACCTAGGGCTCTCCTGTTCAGCCTTAACAGGCTCAGC AAATCAGGGCGTGGCTGGACGATTTCCTTGCATCTGAGGGCAGACGCTGCTACCGGAGTG ACCTGGACGTGGCCAGATCTTCTCGCAGGTCACAAGAAGCCAGTGAGCCCTTGCCTTGGT TTCTGGAAGTTCTTTTCCTTGGCTGGATTTACCCAGTGGTTAGGTTGCATTTCTACCCCA TCCAGAACATTCTTGGAAGAGCACCCGGAGCTGAAGCTGTCCCTGATGATGAAGGTGAAA CGTCAGCCCTGGCCATGGCTCCGCTCAGGGCCCCGGTCACCTCCGAGTCACTCTGTTCCT TGACTGTCTTTGTGTTTCTGTACCTCAAGGCACTGAAGCTGGAGGACTCTGTCCATGCCC GTGTCACCCTCGTGTGGGAGCCTCTGGGCTCGGCAGGTCCACATTTCATGAGCTGAGGCG TGGGCCAGGGCCATCTGGAAAGGGAACTCGGCTTTTCCAGAACGTGGTGGATCATCTGTC GGGTGTGTGGTGAACACGTTCAGTTCATCAGGGCCTACGCTCCGGGAAGGGGCCCCCAGC TGTGGCTCTGCCATGCCGGGCTGTGTTTGCAGCTGTCCGAGTCTCCATCCGCCTTTAGAA AACCAGCCACTTCTTTTCATAAGCACTGACAGGGCCCAGCCCACAGCCACAGGTGCGATC AGTGCCTCACGCAGGCAAATGCACTGAAACCCAGGGGCACACGCGCGCAGAGTGAACAGT GAGTTCCCCCGACAGCCCACGACAGCCAGGACTGCCCTCCCCACCCCACCCCACCCCAGG AGCACGGCACACAGTTCAGCCTCTGAGCTGGCTCACACGTGCCATCCCCACCCCGGTGCT CCAGGGAAGGAGGACACGGACCCGACGTGGGAGGTCCTCAGGCAGCAGTGGCGCCTGGTG TCAGGTCTGTCTGGCTGAGTCCCGGGCGTCCCCTGCCATGGCCTGTGCCTTGCATGGAGG CGGCGGTGGCACTGAAGAGATAGCTTTCAAGGGCCCAACACTTTGCACTTCGGCTGGCTG TGAGTTTCTGCTTTGTAGGTTGTGGTCACATTTGCAGGCTGCGGGCAGTGGCACCGACTT GGGCCTCCCTTTCTATGTGGCATATTTATTTATTTAAACACCCCAGGGAGTTACGTGGTA ACAAGGTTGTCCATAAAGAGGTTGCTTCTATATACTAGAGGCCCCAGATGGCCAGGCCTT GGGCTACGTCTGGCTTGCATGGTCTCCCAAGGGAATCAGCCCCATCAACAAAGTTCAAAT CGGGGCAGAGGCTGCACTTGTGCCCCCAGATGTTTCTGAGGAGCCAGACTAGGGCTGGCA TTGCTGTAGAGTGACGGCTGCTGCCCAGAGCGTGTCCCAGACATCACAGCGGGGCTCAGC AGTTCCCACAGCCTCTGCCTGCCTTGGCTAAGCATGAGTTAAGCAGCAAAACGCTCCTCC ATGTCTGGATGGGGCCGGCAGGTCCTGTGTCCCCTGCACCTGGAGGAGAGCAGGCTAGAG GCACAGCGGCCACATGGTGCTGGCTCTGAACGTTGGTTGGTGGCTGGAAAACAGCCCTGC TTCTGAGGGCCGCTCAGTTCTGCACACGAAACCACCTCCTGAGGGCTCAGCTCTGCCCCC GCCCTGGGCTGCAGCCTCTGCACGCAAGCACCAGGCATCCTTTGTGTTGTCAACTCCGTG TAACCAGTAACTACAGCCATTTACAATTGACTCCGTTTCCTTTTGTAGGTTTCCCTGTCT GTCTGTGTTAGTAGAAAAATAAAATCCTATGAAATCTGAAAAAAAAAAAAAAAAAAAAAA A 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC007760 unedited NNAAGTACAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCAGC GCGGGCCGGGCCGGGACGGGGACTGTCGGCTGCAGGCGGCCATGCCCACCAACTTCACCG TGGTGCCCGTGGAGGCTCACGCCGACGGCGGCGGGGACGAGACTGCCGAGCGGACGGAGG CTCCGGGCACCCCCGAGGGCCCCGAGCCCGAGCGCCCCAGCCCGGGAGATGGAAATCCAA GAGAAAACAGCCCATTCCTCAACAATGTCGAGGTGGAACAAGAGAGCTTCTTTGAAGGGA AGAACATGGCACTTTTCGAGGAGGAGATGGACAGTAACCCCATGGTGTCCTCGCTGCTCA ACAAGCTGGCCAACTACACCAACCTGAGCCAGGGCGTGGTGGAGCACGAGGAGGACGAGG AGAGCCGGCGGCGGGAGGCCAAGGCTCCGCGCATGGGCACCTTCATCGGCGTCTACCTGC CGTGCCTGCAGAACATCCTGGGCGTCATCCTCTTCCTGCGCCTGACGTGGATCGTGGGGG TGGCTGGTGTCCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCACATGTACAATGCTGA CCGCCATTTCCATGAGTGCGATCGCTACCAACGGTGTGGTCCCAGCTGGCGGGTCCTACT ACATGATATCGCGCTCGCTGGGACCCGAGTTTGGAGGCGCTGTCGGCCTCTGCTTCTACC TGGGCACGACGTTTGCAGGGGCCATGTATATTTTGGGGACCATCGAGATTTTTCTGACAG GCACGNGAGACGGCGTGGGCAGCCTGGGCCTGGGCTGGCCCAGGGAACAGACGCAGAACA CCTGTCCCCCATGATGCCGCCAAGCTGCCCTGGGGCTCCTACGGAGTTTCTAGC
Restriction Sites	Please inquire
ACCN	BC007760
Insert Size	2701 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC007760.1, AAH07760.1
RefSeq Size	2701 bp
RefSeq ORF	801 bp
Locus ID	10723
Protein Families	Transmembrane
Gene Summary	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.