HEMK1 (BC000781) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HEMK; MTQ1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC000781 edited CAACTATATCTGAGGACCAGAGCCATTTTGGGGCACCAGAGCTTGTGACCTCTCCATCTC CACCCAGCTGGGTCCAGGGGCCACTCTCAGCACTCACCTCAGCAGCTGACATCATAAAGC AGACTTGGGAACCTGGAAGCACTCTGGAGAACCTTTCCCTGAGACATGGAGCTTTGGGGC CGAATGCTGTGGGCCCTCCTGTCTGGCCCAGGGAGGAGGGGAAGTACCCGGGGCTGGGCC TTCAGCTCATGGCAACCCCAACCACCTCTGGCTGGGTTATCCAGTGCCATAGAACTGGTC AGCCACTGGACTGGGGTCTTTGAGAAGAGGGGTATCCCTGAGGCCCGGGAATCCAGTGAG TACATCGTGGCTCATGTCCTTGGAGCCAAAACATTTCAGAGCCTGAGGCCGGCACTTTGG ACCCAGCCCTTGACCTCTCAGCAACTACAGTGTATCCGGGAGCTGAGTAGCCGTCGATTG CAGAGGATGCCGGTGCAGTACATCCTTGGAGAGTGGGACTTCCAGGGGCTCAGCCTAAGG ATGGTGCCCCCAGTGTTTATTCCGCGGCCAGAAACAGAGGAACTGGTTGAGTGGGTGCTG GAAGAGGTGGCCCAGAGGTCCCATGCTGTGGGATCCCCAGGCAGCCCCCTCATTCTGGAG GTGGGCTGCGGATCAGGAGCCATCTCCCTCAGCCTGCTGAGCCAGCTCCCCCAGAGCCGA GTCATTGCTGTGGATAAGCGGGAAGCTGCTATCTCTCTGACCCATGAGAATGCTCAGAGG CTTCGGTTGCAGGACAGGATTTGGATCATCCACCTCGACATGACCTCAGAAAGGAGCTGG ACACACCTGCCCTGGGGCCCCATGGACCTGATTGTCAGCAACCCTCCCTACGTCTTCCAC CAGGACATGGAGCAGCTGGCCCCTGAGATCCGCAGCTATGAAGACCCCGCGGCCCTGGAT GGTGGGGAGGAGGGCATGGACATCATTACCCACATTCTGGCCTTGGCACCCCGGCTCCTG AAAGACTCTGGTAGTATCTTCTTAGAAGTGGACCCAAGGCACCCGGAGCTTGTCAGCAGC TGGCTTCAGAGCCGGCCTGACCTGTACCTTAATCTTGTGGCTGTGCGCAGGGACTTCTGT GGGAGGCCCCGGTTCCTGCATATCCGGAGGTCTGGGCCATAGCATGGCTGCCCTGTGGAT GCCTTGTCAGTGCCGCCAGCCTGACCAGAGGGGAGGTGGATGGCACTTTCCAGAGCCCAG GTTCTTATGGCATTTCCCAGGGTTCTGTGATTTCCCCATGCTCTGCATTTCTAGGATATT TCTAGGACACCTGGATTGGCTCCATCACATCAGAGTGGCTGAGGGCAGTTGCTCTGTGTT GGTGAAATTGCTGTGGGGGTATCGGGGGATATGGCCAGTAAAGTATTGAGAGACTAAAAA AAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000781 unedited NACGTCAGGAATTGTATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGCAACTAT ATCTGAGGACCAGAGCCATTTTGGGGCACCAGAGCTTGTGACCTCTCCATCTCCACCCAG CTGGGTCCAGGGGCCACTCTCAGCACTCACCTCAGCAGCTGACATCATAAAGCAGACTTG GGAACCTGGAAGCACTCTGGAGAACCTTTCCCTGAGACATGGAGCTTTGGGGCCGAATGC TGTGGGCCCTCCTGTCTGGCCCAGGGAGGAGGGGAAGTACCCGGGGCTGGGCCTTCAGCT CATGGCAACCCCAACCACCTCTGGCTGGGTTATCCAGTGCCATAGAACTGGTCAGCCACT GGACTGGGGTCTTTGAGAAGAGGGGTATCCCTGAGGCCCGGGAATCCAGTGAGTACATCG TGGCTCATGTCCTTGGAGCCAAAACATTTCAGAGCCTGAGGCCGGCACTTTGGACCCAGC CCTTGACCTCTCAGCAACTACAGTGTATCCGGGAGCTGAGTAGCCGTCGATTGCAGAGGA TGCCGGTGCAGTACATCCTTGGAGAGTGGGACTTCCAGGGGCTCAGCCTAAGGATGGTGC CCCCAGTGTTTATTCCGCGGCCAGAAACAGANGAACTGGTTGAGTGNGTGCTGGAAAGAG TAGCCCANNAGTCCNATGCTGNTGGATCCCCATGCAGCCCCCTCATTCTGGNNAGTGGGC TGCGGATCAGGAGCCATCTCCCTCAGCCTGCTGAGCCAGCTCCCCCAGAGCGAGTCATTG CTGTGGATAANCCGGGAGCTGCTATCTCTCTTGACCATGAAAATGCTCAANAGGCTTCCG GTGCAAGAAAAGATTTGGAATCATCCCACTCGGACTGGACCTCAAAAGGAGCTGGACACA CCTGGCCTGGGG
Restriction Sites	Please inquire
ACCN	BC000781
Insert Size	1461 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC000781.2, AAH00781.1
RefSeq Size	1452 bp
Locus ID	51409
Protein Families	Druggable Genome
Protein Pathways	Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
Gene Summary	N5-glutamine methyltransferase responsible for the methylation of the glutamine residue in the universally conserved GGQ motif of the mitochondrial translation release factor MTRF1L.[UniProtKB/Swiss-Prot Function]

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