HIF1AN (BC015574) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FIH1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC015574 edited GGCACGAGGAGGCCTTGGGGAACCCACAAGAGGTGGGGCCCTTGTTGAACACAATGATCA AGGGCCGATACAACTAGCCTGCCAGGGGTCAAGGCCTCCTGCCAGGTGACTGCTATCCCG TCCACACCGCTTCATTGATGAGGACAGGAGACTCCAAGCGCTAGTATTGCACGCTGCACT TAATGGACTGGACTCTTGCCATGGCCCAGGAGTCAGGTGTTTGGAGCGAGGCAGGGCAGT TGGCACTCCACTCCTATTTGGAGGGACTTCATACCCTTGCCTCTTGTGCCCCAGCACCTT CTCTCTCTGCCCCCCGCCTAAAGTCCTGCATTCAGTGTGTGGAGTCCCAGCTTTTGGTTG TCATCATGTCTGTGTGTATGTTAGTCTGTCAACTTCGGAATGTGTGCGTGTGTGTGCATG CACACGCATGTATGTATCTGTTCCCTGTTCCTTCTGGGTCAGGCTGTCACTTCCGGCTCT CAGCCCTATCTCCTGCAACCTCAGTGCCTCAGCCTGAGAGAGAGATGAGATGCTCTTGGA CTCCCCACTGCATCTGGGCTGCAGGGCCAGAGCTAGTCTGACCATTAGGTCAGTCTGCCT CCTGACAGTTTTTGCGTAGTCAAGCTCTAGGCGGTATGGGAATGGCTACCGGGACTCTAA TGGGGTGAAAGAGAGGGGAGGCTTGCCTTTGAGAGCCTATATAGCCTTCCTGTGAGAGAG GATTAGATAGGGTTCCAACTGGGCCTACAAGCTCAAGCCATACATAAAAGGACCTTGGGA CATAAGAACCAATGATTGTGCATAAGTTCTAAATTAGAGACACATATAGTTTCTCTCTTT CAGCACCAGCTCTTGCCCCTATGCTGGGTACCAAGGGAGTTCTCCTAGCTGTGGCTTCTC TAGGTTCTAGGGGTGCAAGCCTCTGTGTGTTTGTTTGTGTGTGTCTGTGTGTGCGTATCC ACACTAGGGGTGCAAGCCTCTGGGTGTGTGTGTGTGTGTGCGTGCGTGTGTGTGTGTGTG TCCGTGTGTGTGTGTGTGTGTCCACACTGGCCAGCCTCCCTACTTACCAAGGTTCTCCAC TGCTTACCTTTTCCAGTGGGACAGTACAGTGTGAGCCCCCGGGAAGTACTGCCTGACCTA TCCTAAGCTTTTACACTTGGATTTTAGCCATCATATGTTGGCCAGGTCTCACTGCAGCCT GCCCGAGGCTAACTGGCTAGAGCCTCCAGGCCCTATGATGCTCCCTGCCCAGGCCATATC CTTTATTCCTGCTGAGCTTCCTGGCTGAATAGATGAAATGGGGTCAAGCCCAGGCAGCTC ATTCACTACCTGTGATCCACCTCAGGGCACGGGCAAACACATAGGCTTGCGTCTTAAAGC CAGCTCCTCTGCCAGACCCCGTTGTAATGTGCCACAACACCCTCAATAGTCAGGGCAACT GGTGGAGCATGGAAGTCGAATTTCCTTTTCTGTTAGGAGCTACTCCTGGGAACCCCTCTC AGGGCTGCAGCTTACAGGTGGGCAGCTGTGATTGCACAACTTGAAGGGCCATCATTCACA TCTATTCAGTGGGAGTGGGGTCCCTGGGATTGGGCAGTGTGGTGGCCCTGTGTCTCCTCA CCTCTGCTCCTGTCTTCATCACCTTCTCTCTGGAAGGGAAGAGGAGTTGGAAGGTCTCTG GTTTTCTTTTCTTTTTTTTTTTTTTTGCCAAAGGTTTACTTCCAGCATCTGAGCTCTGGC TCTCACCCCTGAAGCTCAGTTATAGTGCACTGATGAACTGAGAGGATGCGTGTGGATGTG TGTGCATGCCTGAGTGCGTTTTTTGGGGAGGGGTGTTTATTTTTAGTACCCCATTCTGGG GTTCTCTGATGCAGTGTGGATGTGAAGATATGGTACCTTCTCAAGTGTAGCTCTTTCAAA TATAGTCAATGCTGGGAAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015574 unedited GTGATTTGTTATCCCATACCTTATATGGCGGCCGCGACATTCGCACGAGGNAGGCCTTGG GGTAACCCACAAGAGGTGGGGCCCTTGTTGAACACAATGATCAAGGGCCGATACAACTAG CCTGCCAGGGGTCAAGGCCTCCTGCCAGGTGACTGCTATCCCGTCCACACCGCTTCATTG ATGAGGACAGGAGACTCCAAGCGCTAGTATTGCACTCTGCACTTAATGGACTGGACTCTT GCCATGGCCCAGGAGTCAGGTGTTTGGAGCGAGGCAGGGCAGTTGGCACTCCACTCCTAT TTGGAGGGACTTCATACCCTTGCCTCTTGTGCCCCAGCACCTTCTCTCTCTGCCCCCCGC CTAAAGTCCTGCATTCAGTGTGTGGAGTCCCAGCTTTTGGTTGTCATCATGTCTGTGTGT ATGTTAGTCTGTCAACTTCGGAATGTGTGCGTGTGTGTGCATGCACACGCATGTATGTAT CTGTTCCCTGTTCCTTCTGGGTCAGGCTGTCACTTCCGGCTCTCAGCCCTATCTCCTGCA ACCTCAGTGCCTCAGCCTGAGAGAGAGATGAGATGCTCTTGGACTCCCCACTGCATCTGG GCTGCAGGGCCAGAGCTAGTCTGACCATTAGGTCAGTCTGCCTCCTGACAGTTTTTGCGT AGTCAAGCTCTAGGCGGTATGGGAATGGCTACCGGGACTCTAATGGGGTGAAAGAGAGGG GAGGCTTGCCTTTGAGAGCCTATATAGCCTTCCTGTGAGAGAGGATTAGATAGGGTTCCA ACTGGGCCTACAAGCTCAAGCCATACATAAAAGGACCTTGNGACATAAGAACCAATGATT GTGCATAAGTTCTAAATTAGAGACCATATAGTTTCTCTCTTTCACACCAGCTCTTGCCCT ATGCTGGGTACCAAGGNAGTCTCCTAGCTGGGGCTCTCTAGGTCTAGGGTGCAGCCTCTT GTGTTGTTT
Restriction Sites	Please inquire
ACCN	BC015574
Insert Size	1982 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015574.1, AAH15574.1
RefSeq Size	1982 bp
Locus ID	55662
Protein Families	Druggable Genome
Gene Summary	Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.[UniProtKB/Swiss-Prot Function]

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