ethanolamine kinase (ETNK1) (BC006111) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EKI; EKI1; ethanolamine kinase 1; Nbla10396; putative protein product of Nbla10396
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC006111 edited CTCCGACAACAGGAATTTTCTCCGAGAGCGGGCCGGGCTCAGTTCAGCTGCTGTCCAGAC CCGGATCGGCAACAGTGCCGCCTCCAGACGTTCTCCTGCCGCTCGCCCGCCCGTCCCAGC GCCCCCAGCCCTCCCGCGAGGGCGCCCCGGGACGGAAGGATCCACCAGTCTGTCGGCGCC CGCCGTTCTCGTGGTCGCCGTCGCCGTCGTCGTGGTGGTAGTCTCCGCCGTCGCCTGGGC CATGGCCAATTACATCCACGTCCCTCCCGGCTCCCCGGAGGTGCCCAAGCTGAACGTCAC CGTTCAGGATCAGGAGGAGCATCGCTGCCGGGAGGGGGCCCTGAGCCTCCTGCAACACCT GCGGCCTCACTGGGACCCCCAGGAGGTGACCCTGCAGCTCTTCACAGATGGAATCACAAA TAAACTTATTGGCTGTTACGTGGGAAACACCATGGAGGATGTAGTCCTGGTGAGAATTTA TGGCAATAAGACTGAGTTATTAGTCGATCGAGATGAGGAAGTAAAGAGTTTTCGAGTGTT GCAGGCTCATGGGTGTGCACCACAACTCTACTGTACCTTCAATAATGGACTATGCTATGA ATTTATACAAGGAGAAGCACTGGATCCAAAGCATGTCTGCAACCCAGCCATTTTCAGTTT ATCATCGTTGACTCTTTGCAAAGGAAAAACTACAAGATGTTTTGGATTAACCGGCTGCAG AGGGTCAAGGCTTCTGCTTAGTTTTTTCTAGTTAGTGTTTGAGTTGAAGTGTTATACATT TCTTACTGCCGATTGCTTATTACTTAATTGTTCATGTTTTCAGAGTTCTTGCCTATTAAA ATTTTATTAATTTGGTTAACAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC006111 unedited NAAAAAGTTCAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTC CGACAACAGGAATTTTCTCCGAGAGCGGGCCGGGCTCAGTTCAGCTGCTGTCCAGACCCG GATCGGCAACAGTGCCGCCTCCAGACGTTCTCCTGCCGCTCGCCCGCCCGTCCCAGCGCC CCCAGCCCTCCCGCGAGGGCGCCCCGGGACGGAAGGATCCACCAGTCTGTCGGCGCCCGC CGTTCTCGTGGTCGCCGTCGCCGTCGTCGTGGTGGTAGTCTCCGCCGTCGCCTGGGCCAT GGCCAATTACATCCACGTCCCTCCCGGCTCCCCGGAGGTGCCCAAGCTGAACGTCACCGT TCAGGATCAGGAGGAGCATCGCTGCCGGGAGGGGGCCCTGAGCCTCCTGCAACACCTGCG GCCTCACTGGGACCCCCAGGAGGTGACCCTGCAGCTCTTCACAGATGGAATCACAAATAA ACTTATTGGCTGTTACGTGGGAAACACCATGGAGGATGTAGTCCTGGTGAGAATTTATGG CAATAAGACTGAGTTATTAGTCGATCGAGATGAGGAAGTAAAGAGTTTTCGAGTGTTGCA GGCTCATGGGTGTGCACCACAACTCTACTGTACCTTCAATAATGGACTATGCTATGAATT TATACAAGGAGAAGCACTGGATCCAAAGCATGTCTGCAACCCAGCCATTTTCAGTTTATC ATCGTTGACTCTTTGCAAAGGAAAAACTACAAGATGTTTTGGATTAACCGGCTGCAGAAG GTCAAGGCTTCTGCTTAGTTTTTTCTAGTTAGTGTTTGAGTTGAAAGTGTATACATTTCT TACTGCCGATTGCTTATTACTTAATTGTTCATGTTTTTCAAAGTTCTTGCCTATAA
Restriction Sites	Please inquire
ACCN	BC006111
Insert Size	878 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006111.1, AAH06111.2
RefSeq Size	878 bp
Locus ID	55500
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Glycerophospholipid metabolism, Metabolic pathways
Gene Summary	This gene encodes an ethanolamine kinase, which functions in the first committed step of the phosphatidylethanolamine synthesis pathway. This cytosolic enzyme is specific for ethanolamine and exhibits negligible kinase activity on choline. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

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