FAM125B (BC000122) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	family with sequence similarity 125, member B; FLJ00001; RGD1309709
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC000122 edited CTGCGGCGCCGGCTCCTGCCGCCTGGGCCCCGGGCCCGGCCCCTCCCGCGCCGCCCGGGC GATGAGAAGCTGCTTCTGCGTGAGACGGAGCCGGGACCCGCCGCCGCCGCAGCCACCGCC GCCGCCGCCCCAGCGGGGAACAGACCAGTCCACCATGCCTGAAGTCAAAGACCTCTCAGA AGCCTTGCCAGAAACGTCAATGGATCCCATCACGGGAGTCGGGGTGGTGGCTTCTCGGAA CCGAGCCCCGACAGGCTATGACGTAGTTGCACAGACAGCAGATGGTGTGGATGCTGACCT CTGGAAAGACGGCTTATTTAAATCCAAGGTTACCAGATACCTGTGTTTCACAAGATCATT TTCCAAAGAAAATAGTCATCTGGGGAACGTGTTAGTAGATATGAAGCTCATTGACATCAA GGACACACTGCCTGTGGGCTTCATCCCAATTCAGGAGACGGTGGACACACAGGAAGTGGC TTTTAGGAAGAAGAGGCTGTGCATTAAATTTATTCCACGGGATTCAACGGAAGCTGCGAT TTGTGACATTCGGATCATGGGCCGGACCAAGCAGGCCCCGCCTCAGTACACGTTTATTGG GGAACTGAACAGCATGGGGATCTGGTATCGAATGGGCAGAGTACCAAGAAATCATGACTC ATCTCAACCCACAACGCCTTCCCAGTCATCAGCTGCCTCCACCCCAGCCCCCAACCTTCC CAGGCACATCTCCCTAACACTTCCTGCCACCTTCCGAGGCAGGAACAGCACCCGGACGGA CTACGAGTACCAGCACTCCAATTTGTATGCCATATCAGCAATGGATGGTGTGCCTTTTAT GATTTCAGAGAAGTTTTCTTGTGTTCCAGAAAGTATGCAGCCCTTTGATCTCCTGGGAAT CACCATCAAATCTCTAGCAGAAATCGAAAAAGAGTACGAGTACAGCTTCCGCACAGAGCA GAGCGCAGCCGCCAGGCTCCCGCCCAGCCCCACCAGGTGTCAGCAGATCCCGCAGTCCTG AGGAGCCAGCGGCCACCTGCGGGGAGACCACCGCCGCCCAGACTACTGACGGCAGGGGCT GCTGCCCCCGCCTCCTCCTGCCGCCTCCGCCAGCCCTCCCTCCCACACTGCCCCAGCAGG GCTGGCCCGGAGACTGGGCAGCTAAGTGGGCGCATCCTGTCTTCAGCTGGCCTGACTGAC ACCCCGGCCTCCCTGGGGACATTGTTCATAACCATGACTAATCTGTGTGTGCTGTAGTGA CCAGCGGCTCCTAACGTGTCTGTGTGATGACCAGTTGTCCTCCAAAAACCTCACTCCTGG CAGGTGGGGGCAGCAGGAGGGCAGAGGGCAGAGCTCTGGCCACTTCTGCCCACTTCATTA GGGTTTGTGAACTTTGTCCTTCACCTCTTTTCGTGCCCTGGTTGTGAGATTGCCTCTAAC AGGTAATGCCAGGGGCCCTTCACTCCGCCCCCATGACTGGGAAGAGGCCTGTGGCAGCGC CGCTGGACCCTAGGAGGCTCAGAGGCAGTGGTGTGGGAGCCCTGTCTGCAAGGACGCAGA ATAAGCAGTGAGGGCGGCTGCAGGAGAGGAAGGGGCTCCCACAGCCCCCACTGATGCCGC TGCAGGCCCCTGTCGAGCTGGGGTCCCAGCCAGGTGCCCCCGCATGCCCTCCTGCAGTTG CTGGATGGATAGGGACACCAGGAAGAGGACAAACTGCATGGACTCAAGCGAGCTGGAGCC ATCTTCTCCATAGCATTACGGACTTAGCATAAGAGTAAATGACTGTGAACGTTGTAGTAA ACGGCAGCTTAAGATAAGTAAGCAGAGACAGTGTTAAGGACGAGTTGGTGTCTGTGGTAG CTTTTAGGCTGCTCCTAACCCACCATTTATTGCCTTCTGAGAGGTGGGTGAGGACAAGCA TGTGCCTGTGTGTGTGTGTGTGTGTGTGTGTATATGTGTGTGTGTGCACGCACATGCGTG TGTATAAGCCCACCTGAGTGGGGCTCGTGCAGGAGAACTGAGGCATGAAACTCTGGCTCA AACCTAGGAATTGAGAGCGTTTCTGTCTTTTGGGAGAGTACTTTTCTCCACGAGCCCTCT GGCCACTGTGGGAGGGAAGGACAAGGGTTCCCTTGGAAATGTGAAGGGTCTTGGCCTCAT CCCTCAGGTCCCCCCACAGCACTTCCCACTACTGCTTCTGTCCCTGGACAAGGAAGACCC TTAGGGATGACGTCCCCGCTGCATATTTATTCAAGGTGACTCTTGTACTTGGCAAGGGAA GTCCACTGTGTGATTGTCTGTATTCTTAATATAATTTGTTAAATAAACGTTTGTTTTAAC CTCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000122 unedited NCCAGGTACAGATATTTGTAATACGACTCACTATAGGGGGCGGCCGCGAATTCGCACGAG GCTGCGGCGCCGGCTCCTGCCGCCTGGGCCCCGGGCCCGGCCCCTCCCGCGCCGCCCGGG CGATGAGAAGCTGCTTCTGCGTGAGACGGAGCCGGGACCCGCCGCCGCCGCAGCCACCGC CGCCGCCGCCCCAGCGGGGAACAGACCAGTCCACCATGCCTGAAGTCAAAGACCTCTCAG AAGCCTTGCCAGAAACGTCAATGGATCCCATCACGGGAGTCGGGGTGGTGGCTTCTCGGA ACCGAGCCCCGACAGGCTATGACGTAGTTGCACAGACAGCAGATGGTGTGGATGCTGACC TCTGGAAAGACGGCTTATTTAAATCCAAGGTTACCAGATACCTGTGTTTCACAAGATCAT TTTCCAAAGAAAATAGTCATCTGGGGAACGTGTTAGTAGATATGAAGCTCATTGACATCA AGGACACACTGCCTGTGGGCTTCATCCCAATTCAGGAGACGGTGGACACACAGGAAGTGG CTTTTAGGAAGAAGAGGCTGTGCATTAAATTTATTCCACGGGATTCAACGGAAGCTGCGA TTTGTGACATTCGGATCATGGGCCGGACCAAGCAGGCCCCGCCTCAGTACACGTTTATTG GGGAACTGAACAGCATGGGGATCTGGTATCGAATGGGCAGAGTACCAAGAAATCATGACT CATCTCAACCCACAACGCCCTTCCAGTCATCAGCTGCCTCCACCCCAGCCCCCAACCTTC CCAGGCACATCTCCCTAACACTTCCTGCCACCCTTCCGAGGGCAGGACAGCACCCGGACG GACTACGAGTACCAGCACTCCCATTTGGTATGCCATATCAGCAATGGGATGGTGTGCCCT TTTATGATTTCCAC
Restriction Sites	Please inquire
ACCN	BC000122
Insert Size	2383 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC000122.1
RefSeq Size	2383 bp
Locus ID	89853
Protein Pathways	Endocytosis
Gene Summary	The protein encoded by this gene is a component of the ESCRT-I complex, a heterotetramer, which mediates the sorting of ubiquitinated cargo protein from the plasma membrane to the endosomal vesicle. ESCRT-I complex plays an essential role in HIV budding and endosomal protein sorting. Depletion and overexpression of this and related protein (MVB12A) inhibit HIV-1 infectivity and induce unusual viral assembly defects, indicating a role for MVB12 subunits in regulating ESCRT-mediated virus budding. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

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