BRSK1 (BC016681) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BR serine/threonine kinase 1; FLJ43009; KIAA1811; protein kinase SAD1A; SAD1 kinase
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC016681 edited GACTAGTTCTAGATCGCGAGCGGCTGGGAGGGGTGGGATGCCAGGGTTCCTGAGAGGCAA CGGGCTAGGGACTCGGACTTATGGGTCCTGGGGGAAGAGGACACAGCTAGAGAAGGAGTC TAGGGGTCAGAGGCAGGAGGGGCTAAGCTAGGAGTCCAGGACTCCCAGAGGCGGGAAACA CGAGCCAGACCCGTGCCTGGAGCCAGCCCCTGGCCGCCGGGTAGCCATGCGGAGCCTGCC ATCCAACGGAGAGCTGGACCCCGACGTCCTAGAGAGCATGGCATCACTGGGCTGCTTCAG GGACCGCGAGAGGCTGCATCGCGAGCTGCGCAGTGAGGAGGAGAACCAAGAAAAGATGAT ATATTATCTGCTTTTGGATCGGAAGGAGCGGTATCCCAGCTGTGAGGACCAGGACCTGCC TCCCCGGAATGATGTTGACCCCCCCCGGAAGCGTGTGGATTCTCCCATGCTGAGTCGTCA CGGGAAGCGGCGACCAGAGCGGAAGTCCATGGAAGTCCTGAGCATCACCGATGCCGGGGG TGGTGGCTCCCCTGTACCCACACGACGGGCCTTGGAGATGGCCCAGCACAGCCAGAGATC CCGTAGCGTCAGTGGAGCCTCCACGGGTCTGTCCTCCAGCCCTCTAAGCAGCCCAAGGAG TCCGGTCTTTTCCTTTTCACCGGAGCCGGGGGCTGGAGATGAGGCTCGAGGCGGGGGCTC CCCGACTTCCAAAACGCAGACGCTGCCTTCTCGGGGCCCCAGGGGTGGGGGCGCCGGGGA GCAGCCCCCGCCCCCCAGTGCCCGCTCCACACCCCTGCCCGGCCCCCCAGGCTCCCCGCG CTCCTCTGGCGGGACCCCCTTGCACTCGCCTCTGCACACGCCCCGGGCCAGTCCCACCGG GACCCCGGGGACAACACCACCCCCCAGCCCCGGCGGTGGCGTCGGGGGAGCCGCCTGGAG GAGTCGTCTCAACTCCATCCGCAACAGCTTCCTGGGCTCCCCTCGCTTTCACCGGCGCAA GATGCAGGTCCCTACCGCTGAGGAGATGTCCAGCTTGACGCCAGAGTCCTCCCCGGAGCT GGCAAAACGCTCCTGGTTCGGGAACTTCATCTCCTTGGACAAAGAAGAACAAATATTCCT CGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCAGACATCGTCCATGCCTTTCTGTC GATCCCCAGCCTGAGTCACAGTGTGCTGTCACAGACCAGCTTCAGGGCCGAGTACAAAGC CAGTGGCGGCCCCTCCGTCTTCCAAAAGCCCGTCCGCTTCCAGGTGGACATCAGCTCCTC TGAGGGTCCAGAGCCCTCCCCGCGACGGGACGGCAGCGGAGGTGGTGGCATCTACTCCGT CACCTTCACTCTCATCTCGGGTCCCAGCCGTCGGTTCAAGCGAGTGGTGGAGACCATCCA GGCACAGCTCCTGAGCACTCATGACCAGCCCTCCGTGCAGGCCCTGGCAGACGAGAAGAA CGGGGCCCAGACCCGGCCTGCTGGTGCCCCACCCCGAAGCCTGCAGCCCCCACCCGGCCG CCCAGACCCAGAGCTGAGCAGCTCTCCCCGCCGAGCCCCCCCCAAGGACAAGAAGCTCCT GGCCACCAACGGGACCCCTCTGCCCTGACCCCACGGGGCCGGGGAGGGAGGGGACCCCCC TCCACCCCCCTTCCGTGCCCCCCAACTGTGAATCTGTAAATAAGGCCCAAGGAACATGTC GGGAGGGGGGTGGACACAAAAACCGGCCTTGCCCTGCAGGGATGGGGCTCCACAGGCCGT GCCCAACTGGGGGTGGTTCTAGGGGAACAGGGGGCGGGGGAGCTGTTTCTATTTTATTTA TTGATTAATTTATTATTTTATTTATTGATCAATCTCTCTGCGGGGTGGGGTGGGGGAGGG ACGGGAGCTGGTTGGGGTGGCTTAGCAGATCCGGACAGGGCCCTCTGTCCCTGTGTCGTC CCCAACCCCCTCTTCCCGGGCCCCTCCTCCCCTGGTCCTCCCCCCACGACCTTCTGTACG GATTTGCTCTCCGGAAGGAATTCTGGTTTCGCGTGATCCTGCCTGCGTCCGTGTCTCTGA TTCCGCCGGCGGCAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC016681 unedited GGTTACGATTTGTATACGACTTATATAGGCGGCCGACGATTCCCGGGATATCGTCGACCC ACGCGTCCGGACTAGTTCTAGATCGCGAGCGGCTGGGAGGGGTGGGATGCCAGGGTTCCT GAGAGGCAACGGGCTAGGGACTCGGACTTATGGGTCCTGGGGGAAGAGGACACAGCTAGA GAAGGAGTCTAGGGGTCAGAGGCAGGAGGGGCTAAGCTAGGAGTCCAGGACTCCCAGAGG CGGGAAACACGAGCCAGACCCGTGCCTGGAGCCAGCCCCTGGCCGCCGGGTAGCCATGCG GAGCCTGCCATCCAACGGAGAGCTGGACCCCGACGTCCTAGAGAGCATGGCATCACTGGG CTGCTTCAGGGACCGCGAGAGGCTGCATCGCGAGCTGCGCAGTGAGGAGGAGAACCAAGA AAAGATGATATATTATCTGCTTTTGGATCGGAAGGAGCGGTATCCCAGCTGTGAGGACCA GGACCTGCCTCCCCGGAATGATGTTGACCCCCCCCGGAAGCGTGTGGATTCTCCCATGCT GAGTCGTCACGGGAAGCGGCGACCAGAGCGGAAGTCCATGGAAGTCCTGAGCATCACCGA TGCCGGGGGTGGTGGCTCCCCTGTACCCACACGACGGGGCCTTGGAGATGGCCCAGCACA GCCAGAGATCCCGTACCGTCAGTGGAGCCTCCACGGGTCTGTCCTCCAGCCCTCTAAGCA GCCAANGGNAGTCCGGTCTTTTCCTTTTCACCGNAACCCGGGGCTGGAGATGAGGCTCGA GGCGGGGGGCTCCCCGACTTCCAAAACGCAGACGCTGCCCTTCTCGGGGCCCCAAGGGTG GGGGCGCCCGGGGAGCAACCCCCGCCCCCCAATGCCCGGTTCACAACCCCTGGCGGGGCC CCCAGGCTACCCGGGCTTCTCTGGGGGGGAACCCTT
Restriction Sites	Please inquire
ACCN	BC016681
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC016681.1, AAH16681.1
RefSeq Size	2128 bp
Locus ID	84446
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.[UniProtKB/Swiss-Prot Function]

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