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Smoothened (SMO) (NM_005631) Human Untagged Clone
CAT＃: SC122724
SMO (untagged)-Human smoothened, frizzled family receptor (SMO)
CNY 8,656.00
| Cited in 7 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CRJS; FZD11; Gx; PHLS; SMOH |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005631 edited
GGGCTGAAGACAACTTGGATTGCGAGGCTAGGGCTTGGGGAGTCGTGCATCCCGTTCCGG GCCTCCGCAGCCCAACATGGGCCCCGGGTTCCAAAGTTTGCGAAGTTGGGCGCCGAGGGG CCGGGGCGCGCGGAGCGTCCGGGGGGGCCCGGGCCCGGATTCTCTGGGCGCACAGGTCGC CTGAGCCGCCTCCGCGGCCGCCGAGGTCGTGCGTGTGGCCGGGGGGCTCCGAGGAGCAGG CGGGGGCGCCGGGGCTTTTGCTGAGTTGGCGGGGTTGGCCATGGCCGCTGCCCGCCCAGC GCGGGGGCCGGAGCTCCCGCTCCTGGGGCTGCTGCTGCTGCTGCTGCTGGGGGACCCGGG CCGGGGGGCGGCCTCGAGCGGGAACGCGACCGGGCCTGGGCCTCGGAGCGCGGGCGGGAG CGCGAGGAGGAGCGCGGCGGTGACTGGCCCTCCGCCGCCGCTGAGCCACTGCGGCCGGGC TGCCCCCTGCGAGCCGCTGCGCTACAACGTGTGCCTGGGCTCGGTGCTGCCCTACGGGGC CACCTCCACACTGCTGGCCGGAGACTCGGACTCCCAGGAGGAAGCGCACGGCAAGCTCGT GCTCTGGTCGGGCCTCCGGAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTG TGCCGTATACATGCCCAAGTGTGAGAATGACCGGGTGGAGCTGCCCAGCCGTACCCTCTG CCAGGCCACCCGAGGCCCCTGTGCCATCGTGGAGAGGGAGCGGGGCTGGCCTGACTTCCT GCGCTGCACTCCTGACCGCTTCCCTGAAGGCTGCACGAATGAGGTGCAGAACATCAAGTT CAACAGTTCAGGCCAGTGCGAAGTGCCCTTGGTTCGGACAGACAACCCCAAGAGCTGGTA CGAGGACGTGGAGGGCTGCGGCATCCAGTGCCAGAACCCGCTCTTCACAGAGGCTGAGCA CCAGGACATGCACAGCTACATCGCGGCCTTCGGGGCCGTCACGGGCCTCTGCACGCTCTT CACCCTGGCCACATTCGTGGCTGACTGGCGGAACTCGAATCGCTACCCTGCTGTTATTCT CTTCTACGTCAATGCGTGCTTCTTTGTGGGCAGCATTGGCTGGCTGGCCCAGTTCATGGA TGGTGCCCGCCGAGAGATCGTCTGCCGTGCAGATGGCACCATGAGGCTTGGGGAGCCCAC CTCCAATGAGACTCTGTCCTGCGTCATCATCTTTGTCATCGTGTACTACGCCCTGATGGC TGGTGTGGTTTGGTTTGTGGTCCTCACCTATGCCTGGCACACTTCCTTCAAAGCCCTGGG CACCACCTACCAGCCTCTCTCGGGCAAGACCTCCTACTTCCACCTGCTCACCTGGTCACT CCCCTTTGTCCTCACTGTGGCAATCCTTGCTGTGGCGCAGGTGGATGGGGACTCTGTGAG TGGCATTTGTTTTGTGGGCTACAAGAACTACCGATACCGTGCGGGCTTCGTGCTGGCCCC AATCGGCCTGGTGCTCATCGTGGGAGGCTACTTCCTCATCCGAGGAGTCATGACTCTGTT CTCCATCAAGAGCAACCACCCCGGGCTGCTGAGTGAGAAGGCTGCCAGCAAGATCAACGA GACCATGCTGCGCCTGGGCATTTTTGGCTTCCTGGCCTTTGGCTTTGTGCTCATTACCTT CAGCTGCCACTTCTACGACTTCTTCAACCAGGCTGAGTGGGAGCGCAGCTTCCGGGACTA TGTGCTATGTCAGGCCAATGTGACCATCGGGCTGCCCACCAAGCAGCCCATCCCTGACTG TGAGATCAAGAATCGCCCGAGCCTTCTGGTGGAGAAGATCAACCTGTTTGCCATGTTTGG AACTGGCATCGCCATGAGCACCTGGGTCTGGACCAAGGCCACGCTGCTCATCTGGAGGCG TACCTGGTGCAGGTTGACTGGGCAGAGTGACGATGAGCCAAAGCGGATCAAGAAGAGCAA GATGATTGCCAAGGCCTTCTCTAAGCGGCACGAGCTCCTGCAGAACCCAGGCCAGGAGCT GTCCTTCAGCATGCACACTGTGTCCCACGACGGGCCCGTGGCGGGCTTGGCCTTTGACCT CAATGAGCCCTCAGCTGATGTCTCCTCTGCCTGGGCCCAGCATGTCACCAAGATGGTGGC TCGGAGAGGAGCCATACTGCCCCAGGATATTTCTGTCACCCCTGTGGCAACTCCAGTGCC CCCAGAGGAACAAGCCAACCTGTGGCTGGTTGAGGCAGAGATCTCCCCAGAGCTGCAGAA GCGCCTGGGCCGGAAGAAGAAGAGGAGGAAGAGGAAGAAGGAGGTGTGCCCGCTGGCGCC GCCCCCTGAGCTTCACCCCCCTGCCCCTGCCCCCAGTACCATTCCTCGACTGCCTCAGCT GCCCCGGCAGAAATGCCTGGTGGCTGCAGGTGCCTGGGGAGCTGGGGACTCTTGCCGACA GGGAGCGTGGACCCTGGTCTCCAACCCATTCTGCCCAGAGCCCAGTCCCCCTCAGGATCC ATTTCTGCCCAGTGCACCGGCCCCCGTGGCATGGGCTCATGGCCGCCGACAGGGCCTGGG GCCTATTCACTCCCGCACCAACCTGATGGACACAGAACTCATGGATGCAGACTCGGACTT CTGAGCCTGCAGAGCAGGACCTGGGACAGGAAAGAGAGGAACCAATACCTTCAAGGCTCT TCTTCCTCACCGAGCATGCTTCCCTAGGATCCCGTCTTCCAGAGAACCTGTGGGCTGACT GCCCTCCGAAGAGAGTTCTGGATGTCTGGCTCAAAGCAGCAGGACTGTGGGAAAGAGCCT AACATCTCCATGGGGAGGCCTCACCCCAGGGACAGGGCCCTGGAGCTCAGGGTCCTTGTT TCTGCCCTGCCAGCTGCAGCCTGGTTGGCAGCATCTGCTCCATCGGGGCAGGGGGTATGC AGAGCTTGTGGTGGGGCAGGAACGGTGGAGGCAGAGGTGACAGTTCCCAGAGTGGGCTTT GGTGGCCAGGGAGGCAGCCTAGCCTATGTCTGGCAGATGAGGGCTGGCTGCCGTTTTCTG GGCTGATGGGTGCCCTTTCCTGGCAGTCTCAGTCCAAAAGTGTTGACTGTGTCATTAGTC CTTTGTCTAAGTAGGGCCAGGGCACCGTATTCCTCTCCCAGGTGTTTGTGGGGCTGGAAG GACCTGCTCCCACAGGGGCCATGTCCTCTCTTAATAGGTGGCACTACCCCAAACCCATCT TTTGTTCTCCTATATCCTCCTTCTCCTGTTCCATTTCAGTTCAGTTTCAGCGGTGCCAAC CTCTTTGCGTTTCCTTTTTGTTGATGAGGACCCAGAGCTGCTGCACACACTCACCTCTAA CCCCCTCCCCTCGCTGCTGGGCCCCATCTCCACAGGAGAGACTGGTTCGGCTCTAGGGCC TCAGTCTGGAGTGGGATAGGAGCAGTGAGTGACAAAGCCTCTGAAAGATGCATCATCTCT TCCTCACACCCATTTAGTGGGGGATGGGTCCTCTAGACTTGAGGGGCTACCCTGGGAAGC TGCCGTAGCTTCAGCCAGGCAAGAAAGCTTCCTTCAACCTGCATAGCCGGTGGGTGAGGA GATTCCCACCTTCCATAGCCTCCAAACATGTTCCCAAGGCCCCACTTTCAAGAATCAGAC AGCAGGAAGCCATAGATGCTGGCTGGGTTCCAGGTTATGGGGAGAAGAAATACAGTCAAT AAAAGGTTTTTGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_005631 unedited
NNNNAAATGGCAACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAG GGCTGAAGACAACTTGGATTGCGAGGCTAGGGCTTGGGGAGTCGTGCATCCCGTTCCGGG CCTCCGCAGCCCAACATGGGCCCCGGGTTCCAAAGTTTGCGAAGTTGGGCGCCGAGGGGC CGGGGCGCGCGGAGCGTCCGGGGGGGCCCGGGCCCGGATTCTCTGGGCGCACAGGTCGCC TGAGCCGCCTCCGCGGCCGCCGAGGTCGTGCGTGTGGCCGGGGGGCTCCGAGGAGCAGGC GGGGGCGCCGGGGCTTTTGCTGAGTTGGCGGGGTTGGCCATGGCCGCTGCCCGCCCAGCG CGGGGGCCGGAGCTCCCGCTCCTGGGGCTGCTGCTGCTGCTGCTGCTGGGGGACCCGGGC CGGGGGGCGGCCTCGAGCGGGAACGCGACCGGGCCTGGGCCTCGGAGCGCGGGCGGGAGC GCGAGGAGGAGCGCGGCGGTGACTGGCCCTCCGCCGCCGCTGAGCCACTGCGGCCGGGCT GCCCCCTGCGAGCCGCTGCGCTACAACGTGTGCCTGGGCTCGGTGCTGCCCTACGGTGCC ACCTCCACACTGCTGGCCGGAGACTCGGACTCCCAGGAGGAAGCGCACGGCAAGCTCGTG CTCTGGTCGGGCCTCCGGAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTGT GCCGTATACATGCCCAAGTGTGAGAATGACCGGGTGGAGCTGCCCAGCCGTACCCTCTGC CATGCCACCCGATGCCCCTGTGCCATCNTGGAAAGGGAGCGGGGCTGGCCTGACTTCCTG CGCTGCACTCCTGACCGCTTCCCG |
| Restriction Sites | Please inquire |
| ACCN | NM_005631 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005631.3, NP_005622.1 |
| RefSeq Size | 3772 bp |
| RefSeq ORF | 2364 bp |
| Locus ID | 6608 |
| UniProt ID | Q99835 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| Gene Summary | The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Rare hypomorphic human variation in the heptahelical domain of SMO contributes to holoprosencephaly phenotypes
,Nagai-Tanima, M;Hong, S;Hu, P;Carrington, B;Sood, R;Roessler, E;Muenke, M;,
Hum. Mutat.
,PubMed ID 32906187
[SMO]
|
|
Segmental basal cell naevus syndrome caused by an activating mutation in smoothened.
,null,
The British journal of dermatology
,PubMed ID 26822128
[SMO]
|
|
Identification of recurrent SMO and BRAF mutations in ameloblastomas
,Sweeney, RT;McClary, AC;Myers, BR;Biscocho, J;Neahring, L;Kwei, KA;Qu, K;Gong, X;Ng, T;Jones, CD;Varma, S;Odegaard, JI;Sugiyama, T;Koyota, S;Rubin, BP;Troxell, ML;Pelham, RJ;Zehnder, JL;Beachy, PA;Pollack, JR;West, RB;,
Nat. Genet.
,PubMed ID 24859340
[SMO]
|
|
Synthesis and biological evaluation of novel benzamide derivatives as potent smoothened antagonists
,Wu, TM;Wang, DC;Xiang, P;Zhang, JN;Sang, YX;Lin, HJ;Chen, J;Xie, G;Song, H;Zhao, YL;Xie, YM;,
Bioorg. Med. Chem. Lett., Feb 2014.
,PubMed ID 24491459
[SMO]
|
|
Structural insights into the role of the Smoothened cysteine-rich domain in Hedgehog signalling
,Rana, R;Carroll, CE;Lee, HJ;Bao, J;Marada, S;Grace, CR;Guibao, CD;Ogden, SK;Zheng, JJ;,
Nat Commun Dec 2013.
,PubMed ID 24351982
[SMO]
|
|
Structure of the human smoothened receptor bound to an antitumour agent
,Chong Wang, Huixian Wu, Vsevolod Katritch, Gye Won Han, Xi-Ping Huang, + et al.,
Nature 497, 338-343 doi:10.1038/nature12167
,PubMed ID 23636324
[SMO]
|
|
Selective down-regulation of glioma-associated oncogene 2 inhibits the proliferation of hepatocellular carcinoma cells
,Y Kim, JW Yoon, X Xiao, NM Dean, BP Monia, and EG Marcusson,
Cancer Res. 2007 Apr 15;67(8):3583-93.
[SMO]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203451 | SMO (Myc-DDK-tagged)-Human smoothened, frizzled family receptor (SMO) |
CNY 8,648.00 |
|
| RC203451L1 | Lenti ORF clone of Human smoothened, frizzled family receptor (SMO), Myc-DDK-tagged |
CNY 11,048.00 |
|
| RC203451L2 | Lenti ORF clone of Human smoothened, frizzled family receptor (SMO), mGFP tagged |
CNY 7,700.00 |
|
| RC203451L3 | Lenti ORF clone of Human smoothened, frizzled family receptor (SMO), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC203451L4 | Lenti ORF clone of Human smoothened, frizzled family receptor (SMO), mGFP tagged |
CNY 11,048.00 |
|
| RG203451 | SMO (tGFP-tagged) - Human smoothened, frizzled family receptor (SMO) |
CNY 10,248.00 |
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