United States
Germany
Japan
United Kingdom
China
GPBAR1 (NM_170699) Human Untagged Clone
CAT＃: SC123312
GPBAR1 (untagged)-Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3
CNY 2,400.00
CNY 2,950.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BG37; GPCR19; GPR131; M-BAR; TGR5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001077194 edited
GCAGAGCCTGGGTAGGAGAGCCTGGCCCCGCTGTCCCCACTGGGTGGAGACACCATGCAC TTGGTCCTTGTGCTCTTCAGCCAGGACACCAGACATGGTCCAAACCGCTGCAGGGCTGGC TGCAGCAACTCCCTGACACTCAGGAAGGCCCAGGCTGGGCAGGCAATACCTGCTCCCAAC AGCCATGCATGCCGGCTGCCGCTCCAGGACTCCCCTGTCCCCAGGACCAAGATGACGCCC AACAGCACTGGCGAGGTGCCCAGCCCCATTCCCAAGGGGGCTTTGGGGCTCTCCCTGGCC CTGGCAAGCCTCATCATCACCGCGAACCTGCTCCTAGCCCTGGGCATCGCCTGGGACCGC CGCCTGCGCAGCCCACCTGCTGGCTGCTTCTTCCTGAGCCTACTGCTGGCTGGGCTGCTC ACGGGTCTGGCATTGCCCACATTGCCAGGGCTGTGGAACCAGAGTCGCCGGGGTTACTGG TCCTGCCTCCTCGTCTACTTGGCTCCCAACTTCTCCTTCCTCTCCCTGCTTGCCAACCTC TTGCTGGTGCACGGGGAGCGCTACATGGCAGTCCTGAGGCCACTCCAGCCCCCTGGGAGC ATTCGGCTGGCCCTGCTCCTCACCTGGGCTGGTCCCCTGCTCTTTGCCAGTCTGCCCGCT CTGGGGTGGAACCACTGGACCCCTGGTGCCAACTGCAGCTCCCAGGCTATCTTCCCAGCC CCCTACCTGTACCTCGAAGTCTATGGGCTCCTGCTGCCCGCCGTGGGTGCTGCTGCCTTC CTCTCTGTCCGCGTGCTGGCCACTGCCCACCGCCAGCTGCAGGACATCTGCCGGCTGGAG CGGGCAGTGTGCCGCGATGAGCCCTCCGCCCTGGCCCGGGCCCTTACCTGGAGGCAGGCA AGGGCACAGGCTGGAGCCATGCTGCTCTTCGGGCTGTGCTGGGGGCCCTACGTGGCCACA CTGCTCCTCTCAGTCCTGGCCTATGAGCAGCGCCCGCCACTGGGGCCTGGGACACTGTTG TCCCTCCTCTCCCTAGGAAGTGCCAGTGCAGCGGCAGTGCCCGTAGCCATGGGGCTGGGC GATCAGCGCTACACAGCCCCCTGGAGGGCAGCCGCCCAAAGGTGCCTGCAGGGGCTGTGG GGAAGAGCCTCCCGGGACAGTCCCGGCCCCAGCATTGCCTACCACCCAAGCAGCCAAAGC AGTGTCGACCTGGACTTGAACTAAAGGAAGGGCCTCTGCTGACTCCTACCAGAGCATCCG TCCAGCTCAGCCATCCAGCCTGTCTCTACTGGGCCCCACTTCTCTGGATCAGAGACCCTG CCTCTGTTTGACCCCGCACTGACTGAATAAAGCTCCTCTGGCCGTTAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_170699 unedited
TATAGGGCGGCCGAGACTTCCCGGGNTGCAGAGCCTGGTTAGGAGAGCCTGGCCCCGCTG TCCCCCTGGGTGGAGACACCATGCACTTGGTCCTTGTGCTCTTCAGCCAGGACACCAGAC ATGGTCCAAACCGCTGCAGGGCTGGCTGCAGCAACTCCCTGACACTCAGGAAGGCCCAGG CTGGGCTGTTTATACCTGCTCCCAACAGCCATGCATGCCGGCTGCCGCTCCAGGACTCCC CTGTCCCCAGGACCAAGATGACGCCCAACAGCACTGGCGAGGTGCCCAGCCCCATTCCCA AGGGGGCTTTGGGGCTCTCCCTGGCCCTGGCAAGCCTCATCATCACCGCGAACCTGCTCC TAGCCCTGGGCATCGCCTGGGACCGCCGCCTGCGCAGCCCACCTGCTGGCTGCTTCTTCC TGAGCCTACTGCTGGCTGGGCTGCTCACGGGTCTGGCATTGCCCACATTGCCAGGGCTGT GGAACCAGAGTCGCCGGGGTTACTGGTCCTGCCTCCTCGTCTACTTGGCTCCCAACTTCT CCTTCCTCTCCCTGCTTGCCAACCTCTTGCTGGTGCACGGNGAGCGCTACATGGCAGTCC TGAGGCCACTCCAGCCCCCTGGGAGCATTCGGCTGGCCCTGCTCCTCACCTGGGCTGGTC CCCTGCTCTTTGCCAGTCTGCCCGCTCTGGGGTGGAACCACTGGACCCCTGGTGCAACTG CAGCTCCCAGCTATCTTCCCAGCCCCCTACCTGTACCTCGAAGTCTATGGGCTCCTGCTG CCCGCCGTGGGTGCTGCTGCCTTCCTCTCTGTCCGCGTGCTGGCCACTGCCCACCGCCAG CTGCAGGACATCTGCCGGCTGGAGCGGGCAGTGTGCCGCGN |
| Restriction Sites | Please inquire |
| ACCN | NM_170699 |
| Insert Size | 1400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_170699.1, NP_733800.1 |
| RefSeq Size | 1400 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 151306 |
| UniProt ID | Q8TDU6 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Bile acids are important direct and indirect regulators of the secretion of appetite- and metabolism-regulating hormones from the gut and pancreas
,Kuhre, R;Wewer Albrechtsen, N;Larsen, O;Jepsen, S;Balk-Møller, E;Andersen, D;Deacon, C;Schoonjans, K;Reimann, F;Gribble, F;Albrechtsen, R;Hartmann, B;Rosenkilde, M;Holst, J;,
Molecular Metabolism
,PubMed ID 29656109
[GPBAR1]
|
|
Allspice and Clove As Source of Triterpene Acids Activating the G Protein-Coupled Bile Acid Receptor TGR5
,Ladurner, A;Zehl, M;Grienke, U;Hofstadler, C;Faur, N;Pereira, FC;Berry, D;Dirsch, VM;Rollinger, JM;,
Front. Pharmacol.
,PubMed ID 28769799
[GPBAR1]
|
|
Spergularia marina Induces Glucagon-Like Peptide-1 Secretion in NCI-H716 Cells Through Bile Acid Receptor Activation
,Kim, K;Lee, YM;Rhyu, MR;Kim, HY;,
J Med Food
,PubMed ID 25260089
[GPBAR1]
|
|
Ginsenoside metabolite compound K stimulates glucagon-like peptide-1 secretion in NCI-H716 cells via bile acid receptor activation
,Kim, K;Park, M;Lee, YM;Rhyu, MR;Kim, HY;,
Arch. Pharm. Res.
,PubMed ID 24590628
[GPBAR1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221897 | GPBAR1 (Myc-DDK-tagged)-Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3 |
CNY 2,400.00 |
|
| RC221897L1 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC221897L2 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC221897L3 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221897L4 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG221897 | GPBAR1 (tGFP-tagged) - Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3 |
CNY 4,000.00 |
