Hyaluronan synthase 1 (HAS1) (BC035837) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HAS; hyaluronan synthase 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC035837 edited GCAAGCCGGAGAGAAGAGAGAGCCCGGCCAGACCCACTGCGATGAGACAGGACGCGCCCA AGCCCACTCCTGCAGCCCGCCGCTGCTCCGGCCTGGCCCGGAGGGTGCTGACCATCGCCT TCGCCCTGCTCATCCTGGGCCTCATGACCTGGGCCTACGCCGCCGGGGTGCCGCTGGCCT CCGATCGCTACGGCCTCCTGGCCTTCGGCCTCTACGGGGCCTTCCTTTCAGCGCACCTGG TGGCGCAGAGCCTCTTCGCGTACCTGGAGCACCGGCGGGTGGCGGCGGCGGCGCGGGGGC CGCTGGATGCAGCCACCGCGCGCAGTGTGGCGCTGACCATCTCCGCCTACCAGGAGGACC CCGCGTACCTGCGCCAGTGCCTGGCGTCCGCCCGCGCCCTGCTGTACCCGCGCGCGCGGC TGCGCGTCCTCATGGTGGTGGATGGCAACCGCGCCGAGGACCTCTACATGGTCGACATGT TCCGCGAGGTCTTCGCTGACGAGGACCCCGCCACGTACGTGTGGGACGGCAACTACCACC AGCCCTGGGAACCCGCGGCGGCGGGCGCGGTGGGCGCCGGAGCCTATCGGGAGGTGGAGG CGGAGGATCCTGGGCGGCTGGCAGTGGAGGCGCTGGTGAGGACTCGTAGGTGCGTGTGCG TGGCGCAGCGCTGGGGCGGCAAGCGCGAGGTCATGTACACAGCCTTCAAGGCGCTCGGAG ATTCGATGGACTACGTGCAGGTCTGTGACTCGGACACAAGGTTGGACCCCATGGCACTGC TGGAGCTCGTGCGGGTACTGGACGAGGACCCCCGGGTAGGGGCTGTTGGTGGGGACGTGC GGATCCTTAACCCTCTGGACTCCTGGGTCAGCTTCCTAAGCAGCCTGCGATACTGGGTAG CCTTCAATGTGGAGCGGGCTTGTCAGAGCTACTTCCACTGTGTATCCTGCATCAGCGGTC CTCTAGGCCTATATAGGAATAACCTCTTGCAGCAGTTTCTTGAGGCCTGGTACAACCAGA AGTTCCTGGGTACCCACTGTACTTTTGGGGATGACCGGCACCTCACCAACCGCATGCTCA GCATGGGTTATGCTACCAAGTACACCTCCAGGTCCCGCTGCTACTCAGAGACGCCCTCGT CCTTCCTGCGGTGGCTGAGCCAGCAGACACGCTGGTCCAAGTCGTACTTCCGTGAGTGGC TGTACAACGCGCTCTGGTGGCACCGGCACCATGCGTGGATGACCTACGAGGCGGTGGTCT CCGGCCTGTTCCCCTTCTTCGTGGCGGCCACTGTGCTGCGTCTGTTCTACGCGGGCCGCC CTTGGGCGCTGCTGTGGGTGCTGCTGTGCGTGCAGGGCGTGGCACTGGCCAAGGCGGCCT TCGCGGCCTGGCTGCGGGGCTGCCTGCGCATGGTGCTTCTGTCGCTCTACGCGCCCCTCT ACATGTGTGGCCTCCTGCCTGCCAAGTTCCTGGCGCTAGTCACCATGAACCAGAGTGGCT GGGGCACCTCGGGCCGGCGGAAGCTGGCCGCTAACTACGTCCCTCTGCTGCCCCTGGCGC TCTGGGCGCTGCTGCTGCTTGGGGGCCTGGTCCGCAGCGTAGCACACGAGGCCAGGGCCG ACTGGAGCGGCCCTTCCCGCGCAGCCGAGGCCTACCACTTGGCCGCGGGGGCCGGCGCCT ACGTGGGCTACTGGGTGGCCATGTTGACGCTGTACTGGGTGGGCGTGCGGAGGCTTTGCC GGCGGCGGACCGGGGGCTACCGCGTCCAGGTGTGAGTCCAGCCACGCGGATGCCGCCTCA AGGGTCTTCAGGGGAGGCCAGAGGAGAGCTGCTGGGCCCCGAGCCACGAACTTGCTGGGT GGTTCTCTGGGCCTCAGTTTCCCTCCTCTGCAAAACGAGGGGGTCAGCCCAAGATTCTTC AGTCTGGACTATATTGGGACTGGGACTTCTGGGTCTCCAGGGAGGGTATTTATTGGTCAG GGTGTGGGATCTGAGGAGTGGAGGGAAAGGGTCCTGCTTTCTCCTCGTTCTTATTTAATC TCCATTTCTACTGTGTGATCAGGATGTAATAAAGAATTTTATTTATTTTCAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC035837 unedited GGGGTTCGCATTTGTATACGACTCATATAGGCGGCCGCGNATTCCCGGGATAGCAAGCCG GAGAGAAGAGAGAGCCCGGCCAGACCCACTGCGATGAGACAGGACGCGCCCAAGCCCACT CCTGCAGCCCGCCGCTGCTCCGGCCTGGCCCGGAGGGTGCTGACCATCGCCTTCGCCCTG CTCATCCTGGGCCTCATGACCTGGGCCTACGCCGCCGGGGTGCCGCTGGCCTCCGATCGC TACGGCCTCCTGGCCTTCGGCCTCTACGGGGCCTTCCTTTCAGCGCACCTGGTGGCGCAG AGCCTCTTCGCGTACCTGGAGCACCGGCGGGTGGCGGCGGCGGCGCGGGGGCCGCTGGAT GCAGCCACCGCGCGCAGTGTGGCGCTGACCATCTCCGCCTACCAGGAGGACCCCGCGTAC CTGCGCCAGTGCCTGGCGTCCGCCCGCGCCCTGCTGTACCCGCGCGCGCGGCTGCGCGTC CTCATGGTGGTGGATGGCAACCGCGCCGAGGACCTCTACATGGTCGACATGTTCCGCGAG GTCTTCGCTGACGAGGACCCCGCCACGTACGTGTGGGACGGCAACTACCACCAGCCCTGG GAACCCGCGGCGGCGGGCGCGGTGGGCGCCGGAGCCTATCGGGAGGTGGAGGCGGAGGAT CCTGGGCGGCTGGCAGTGGAGGCGCTGGTGAGGACTCGTANGTGCGTGTGCGTGGCGCAC CGCTGGGGCGGCAAGCGCGAGGTCATGTACACAGCCTTCAAGGCGCTCGNAGATTCGATG GACTACGTGCAGGTCTGTGACTCGGACACCAGGTTGGGACCCCATGCACTGCTGGAGCTC GTGCGGGTACTGGACGAANACCCCCGGGTAGGGGCTGTTGGTGGGGACGTGCGGATCCTT AAACCTCTGGACTCN
Restriction Sites	Please inquire
ACCN	BC035837
Insert Size	2119 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC035837.1, AAH35837.1
RefSeq Size	2119 bp
RefSeq ORF	1731 bp
Locus ID	3036
Protein Families	Druggable Genome, Transmembrane
Gene Summary	Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and a recently described murine hyaluronan synthase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

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