Villin (VIL1) (BC017303) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	D2S1471; OTTHUMP00000164145; VIL; villin 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC017303 edited CCAAGACAGGCTCACTCACCATGACCAAGCTGAGCGCCCAAGTCAAAGGCTCTCTCAACA TCACCACCCCGGGGCTGCAGATATGGAGGATCGAGGCCATGCAGATGGTGCCTGTTCCTT CCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTACATCATCCTGGCTATCCACAAGA CAGCCAGCAGCCTGTCCTATGACATCCACTACTGGATTGGCCAGGACTCATCCCTGGATG AGCAGGGGGCAGCTGCCATCTACACCACACAGATGGATGACTTCCTGAAGGGCCGGGCTG TGCAGCACCGCGAGGTCCAGGGCAACGAGAGCGAGGCCTTCCGAGGCTACTTCAAGCAAG GCCTTGTGATCCGGAAAGGGGGCGTGGCTTCTGGCATGAAGCATGTGGAGACCAACTCCT ATGACGTCCAGAGGCTGCTGCATGTCAAGGGCAAGAGGAACGTGGTAGCTGGAGAGGTAG AGATGTCCTGGAAGAGTTTCAACCGAGGGGATGTTTTCCTCCTGGACCTTGGGAAGCTTA TCATCCAGTGGAATGGACCGGAAAGCACCCGTATGGAGAGACTCAGGGGCATGACTCTGG CCAAGGAGATCCGAGACCAGGAGCGGGGAGGGCGCACCTATGTAGGCGTGGTGGACGGAG AGAATGAATTGGCATCCCCGAAGCTGATGGAGGTGATGAACCACGTGCTGGGCAAGCGCA GGGAGCTGAAGGCGGCCGTGCCCGACACGGTGGTGGAGCCGGCACTCAAGGCTGCACTCA AACTGTACCATGTGTCTGACTCCGAGGGGAATCTGGTGGTGAGGGAAGTCGCCACACGGC CACTGACACAGGACCTGCTCAGTCACGAGGACTGTTACATCCTGGACCAGGGGGGCCTGA AGATCTACGTGTGGAAAGGGAAGAAAGCCAATGAGCAGGAGAAGAAGGGAGCCATGAGCC ATGCGCTGAACTTCATCAAAGCCAAGCAGTACCCACCAAGCACACAGGTGGAGGTGCAGA ATGATGGGGCTGAGTCGGCCGTCTTTCAGCAGCTCTTCCAGAAGTGGACAGCGTCCAACC GGACCTCAGGCCTAGGCAAAACCCACACTGTGGGCTCCGTGGGTGAGGGCCAGGCGGGGG CAGTGAGGGAGCCAGGATCCAGGAGCTGGGCCAGGAGAGCCACTTGGTCCACCACCCACC CTCCTTCCCTGACGTGCATCTTCAACGAAGACTTTTACGCTGGCTCTGGGCTTGTCCTAG CAGATGGGGACGTGGACAAACTCTAGTTGTCACAAAAAGGGAGCTGAGAGGGCTGGGCAT GGTGGCACATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAGG TCAGGAGTTTGAGATTAGCCTAACATGGTGAAACCCCGTCTCTACTGAAAAAAAGAACTA ATAAATTAGCCAAGCATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017303 unedited GGCCGATTTGGTTTNTGTTAAACGNACTCACTNANAGGGCGTGCCGCGNAATTCGGCACG GAGGCCAAAACAGGCTCACTCACCATGACCAAGCTGAGCGCCCAATCAAAGCTCTCTCAA CATCACCACCCCGGGGCTGCAGATATGGAGGATCGAGGCCATGCAGATGGTGCCTGTTCC TTCCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTACATCATCCTGGCTATCCACAA GACAGCCAGCAGCCTGTCCTATGACATCCACTACTGGATTGGCCAGGACTCATCCCTGGA TGAGCAGGGGGCAGCTGCCATCTACACCACACAGATGGATGACTTCCTGAAGGGCCGGGC TGTGCAGCACCGCGAGGTCCAGGGCAACGAGAGCGAGGCCTTCCGAGGCTACTTCAAGCA AGGCCTTGTGATCCGGAAAGGGGGCGTGGCTTCTGGCATGAAGCATGTGGAGACCAACTC CTATGACGTCCAGAGGCTGCTGCATGTCAAGGGCAAGAGGAACGTGGTAGCTGGAGAGGT AGAGATGTCCTGGAAGAGTTTCAACCGAGGGGATGTTTTCCTCCTGGACCTTGGGAAGCT TATCATCCAGTGGAATGGACCGGAAAGCACCCGTATGGAGAGACTCAGGGGCATGACTCT GGCCAAGGAGATCCGAGACCAGGAGCGGGGAGGGCGCACCTATGTAGGCGTGGTGGACGG AGAGAATGAATTGGCATCCCCGAAGCTGATGGAGGTGATGAACCACGTGCTGNGCAAGCG CAGGGAGCTGAAGGCGGCCGTGCCCGACACGGTGGTGGAGCCCGCACTCAAGGCTGCACT CAAACTGTACCATGTGTCTGACTCCGAGGNGAAATCTGTGGGTGAGGGAAAGTCGCCACG GCCACTGACACAGACCTGCTCAGTACGA
Restriction Sites	Please inquire
ACCN	BC017303
Insert Size	1501 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC017303.2, AAH17303.1
RefSeq Size	1492 bp
Locus ID	7429
Gene Summary	This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]

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