MT3 (BC013081) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GIF; GIFB; GRIF; metallothionein 3; metallothionein 3 (growth inhibitory factor (neurotrophic))
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC013081 edited TGCTTGGAGAAGCCCGTTCACCGCCTCCAGCTGCTGCTCTCCTCGACATGGACCCTGAGA CCTGCCCCTGCCCTTCTGGTGGCTCCTGCACCTGCGCGGACTCCTGCAAGTGCGAGGGAT GCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCTGCGGAGTGTGAGAAGT GTGCCAAGGACTGTGTGTGCAAAGGCGGAGAGGCAGCTGAGGCAGAAGCAGAGAAGTGCA GCTGCTGCCAGTGAGAAGGCACCCCTCCGTGTGGAGCACGTGGAGATAGTGCCAGGTGGC TCAGTGCCACCTATGCCTGTGGTGAAGTGTGGCTGGTGTCCCCTTCCCCTGCTGACCTTG GAGGAATGACAATAAATCCCATGAACAGCAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC013081 unedited AGGGCGGCAAAAACCTTCCCGGGATATCGTCGACCCACGCGTCCGTGCTTGGAGAAGCCC GTTCACCGCCTCCAGCTGCTGCTCTCCTCGAATGGACCCTGAGACCTGCCCCTGCCCTTC TGGTGGCTCCTGCACCTGCGCGGACTCCTGCAAGTGCGAGGGATGCAAATGCACCTCCTG CAAGAATATTTGCTGCTCCTGCTGCCCTGCGGAGTGTGAGAAGTGTGCCAAGGACTGTGT GTGCAAAGGCGGAGAGGCAGCTGAGGCAGAAGCAGAGAAGTGCAGCTGCTGCCAGTGAGA AGGCACCCCTCCGTGTGGAGCACGTGGAGATAGTGCCAGGTGGCTCAGTGCCACCTATGC CTGTGGTGAAGTGTGGCTGGTGTCCCCTTCCCCTGCTGACCTTGGAGGAATGACAATAAA TCCCATGAACAGCNNANAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA GGGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCT CCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAA TAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGA GGGGGGTGGTAATGGANCAAGGGGCAAGTTGGGGAAGACAACCTGTAAGGCCTGCGGGGT CTATGGGGAACCAAGCTGGAGTGCAGGGGCACAATCTTGGCTTACTGCAATCTCCCGCTC CTGGGTTCAAGCGATTCTCCTGCCTCAACCCTCCGAGTTGTGGGGATTCCAGGCATGGCT GAACAAGCTCAACTAATTTTGGTTTCTTTGGAAAAACGGGGTTTTACCATATTGGCCCAG CCGGGCCCCCAA
Restriction Sites	Please inquire
ACCN	BC013081
Insert Size	400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013081.1, AAH13081.1
RefSeq Size	404 bp
Locus ID	4504
Gene Summary	This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. This gene family member displays tissue-specific expression, and contains a threonine insert near its N-terminus and a glutamate-rich hexapeptide insert near its C-terminus relative to the proteins encoded by other gene family members. It plays an important role in zinc and copper homeostasis, and is induced under hypoxic conditions. The encoded protein is a growth inhibitory factor, and reduced levels of the protein are observed in the brains of individuals with some metal-linked neurodegenerative disorders such as Alzheimer's disease. [provided by RefSeq, Sep 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.