FCHSD2 (BC010394) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FCH and double SH3 domains 2; KIAA0769; nervous wreck homolog; NWK; SH3 multiple domains 3; SH3MD3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC010394 edited GGGAAGCAGAATGTGATTTGCTTGAAGATATGAGGACATTCAGTCAGAAGAAGGCTGCTA TTGAAAGAGAGTATGCACAGTATTCCATTGTATGGATATACCACAGTTTATCCATTTTCC TGTTGATGGACATGGTATGCAGAAGTTGGCTAGTCAATACCTGAAGAGAGATTGGCCTGG AGTAAAAGCTGATGATCGGAATGATTACAGGAGCATGTATCCCGTTTGGAAATCTTTTCT CGAGGGAACAATGCAGGTAGCCCAGTCTCGGATGAATATATGTGAAAACTATAAAAACTT CATTTCTGAGCCTGCAAGGACAGTGAGAAGCTTAAAAGAACAGCAACTAAAAAGGTGTGT GGACCAGTTGACAAAGATCCAAACTGAATTACAAGAGACAGTGAAAGATTTAGCTAAAGG CAAAAAGAAATACTTTGAGACTGAACAGATGGCTCATGCAGTACGAGAGAAAGCTGACAT CGAGGCAAAATCTAAACTTAGTCTTTTTCAATCAAGAATCAGTTTACAGAAGGCAAGTGT AAAGTTAAAAGCCCGGCGATCTGAGTGTAATTCCAAAGCTACCCACGCAAGGAATGATTA TCTTCTTACCCTAGCGGCAGCAAATGCACATCAGGATCGCTACTATCAAACAGATTTAGT TAACATTATGAAGGCTCTTGATGGAAATGTGTATGATCATCTCAAGGATTATTTAATAGC CTTCAGCCGGACTGAGCTAGAAACATGCCAAGCTGTGCAGAACACATTCCAGTTTTTATT AGAAAACTCCAGCAAGGTGGTCCGGGACTACAATCTTCAGCTGTTTTTGCAAGAAAACGC TGTATTTCACAAACCCCAGCCCTTCCAGTTCCAGCCTTGTGACAGTGATACTAGCCGACA GTTAGAATCAGAAACTGGGACCACAGAGGAGCACAGTCTAAATAAGGAAGCTCGAAAATG GGCCACACGTGTGGCACGTGAGCATAAAAACATTGTTCACCAACAACGGGTTCTAAATGA TCTGGAGTGTCATGGAGCTGCTGTATCAGAACAAAGCCGAGCAGAGCTAGAACAGAAAAT AGATGAAGCTAGAGAAAATATTCGTAAAGCAGAGATAATTAAATTGAAAGCTGAAGCCCG GTTGGACCTGCTAAAGCAGATTGGTGTTTCTGTGGACACATGGCTAAAGAGTGCCATGAA CCAAGTAATGGAAGAACTGGAAAATGAGCGATGGGCCCGCCCTCCTGCAGTGACCAGTAA TGGCACTTTACACTCGCTTAATGCAGATACCGAAAGAGAAGAAGGCGAAGAGTTTGAAGA TAACATGGATGTTTTCGATGACAGCAGTTCCAGCCCTTCTGGCACCTTAAGAAATTATCC ACTCACCTGCAAAGTTGTTTATTCCTACAAGGCTTCTCAACCAGATGAGTTGACCATTGA GGAACATGAGGTGTTAGAAGTGATTGAAGATGGAGATATGGAAGACTGGGTAAAGGCTCG AAATAAAGTTGGCCAAGTGGGTTATGTGCCAGAAAAGTACCTACAGTTTCCCACCTCGAA CAGCCTCCTGAGCATGCTGCAGTCCCTGGCCGCTTTGGACAGTCGGTCACACACGTCCAG CAATTCCACGGAAGCAGAACTCGTTTCAGGCAGCCTCAACGGAGATGCCAGTGGTAAAGA CTGAAACAAGGCACTCTTTATTTGGTCACGTTTGACCTAAACTGTATTGCCCTGGAGTAA GCCTTCAGTAGGATAAAAAGCACAACTTTCCTCCTGTCCTATAATATGCTTTTCTTTATA TGGTCACGTTTGTCCTGCACAGTAGAACCCTAAAGTTAATCATTCATCAGTGAGTAAAAA AATTACAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010394 unedited AGCGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCCATTACGGCCGGG AAGCAGAATGTGATTTGCTTGAAGATATGAGGACATTCAGTCAGAAGAAGGCTGCTATTG AAAGAGAGTATGCACAGTATTCCATTGTATGGATATACCACAGTTTATCCATTTTCCTGT TGATGGACATGGTATGCAGAAGTTGGCTAGTCAATACCTGAAGAGAGATTGGCCTGGAGT AAAAGCTGATGATCGGAATGATTACAGGAGCATGTATCCCGTTTGGAAATCTTTTCTCGA GGGAACAATGCAGGTAGCCCAGTCTCGGATGAATATATGTGAAAACTATAAAAACTTCAT TTCTGAGCCTGCAAGGACAGTGAGAAGCTTAAAAGAACAGCAACTAAAAAGGTGTGTGGA CCAGTTGACAAAGATCCAAACTGAATTACAAGAGACAGTGAAAGATTTAGCTAAAGGCAA AAAGAAATACTTTGAGACTGAACAGATGGCTCATGCAGTACGAGAGAAAGCTGACATCGA GGCAAAATCTAAACTTAGTCTTTTTCAATCAAGAATCAGTTTACAGAAGGCAAGTGTAAA GTTAAAAGCCCGGCGATCTGAGTGTAATTCCAAAGCTACCCACGCAAGGAATGATTATCT TCTTACCCTAGCGGCAGCAAATGCACATCANGATCGCTACTATCAAACAGATTTAGTTAA CATTATGAAGGCTCTTGNATGGAATGTGTATGATCATCTCAAGGATATTTAATAGCCTTC AGCCCGACTGAGCTAGAAACATGCCAAGCTGTGCAGAACACATTCCAGTTTTTATTAAAA AACTCCAGGCAGGTGGTCCGGGACTACAATCTTCAGCTGTGTTTGCAGGAAAC
Restriction Sites	Please inquire
ACCN	BC010394
Insert Size	1899 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010394.1, AAH10394.1
RefSeq Size	1899 bp
Locus ID	9873
Gene Summary	Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor (PubMed:29887380). Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling (PubMed:30249660). Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits (PubMed:29887380). Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (PubMed:29887380). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex. Does not promote actin polymerisation in the absence of membranes (PubMed:29887380).[UniProtKB/Swiss-Prot Function]

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