Acylglycerol Kinase (AGK) (BC009775) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	acylglycerol kinase; FLJ10842; MULK; multi-substrate lipid kinase; multiple substrate lipid kinase
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009775 edited GGGGTGGACCAGCCGTGCAAATCTCTAGAAGATGACGGTGTTCTTTAAAACGCTTCGAAA TCACTGGAAGAAAACTACAGCTGGGCTCTGCCTGCTGACCTGGGGAGGCCATTGGCTCTA TGGAAAACACTGTGATAACCTCCTAAGGAGAGCAGCCTGTCAAGAAGCTCAGCACTATCA GGATGAATCACGCTGGGAGCCAACTCTGAGTAGAACTCCAGGAAGTTAACCATCTGGTGA AGAGCAGTTCTGACGCCCACCCCTATAGACTGTTTGTTTCTGGATTGGTCCCAAGAACAC AGAACTGGACTGGTAGAAGCTCTGGGAATTTGTTTGTGTCTGCAGTTGTCTTTGTAGATT TGTCTAACCCCTGAATCATCCCTGATCTGAATAACCCCCAAGGATATCAGTTCAGAGCAA GTGTTACCTCTAAAAGTCAAATCTTCTTAAAATTGTTGATTCATATTTTTAAAGCTCATC TCCAAGTTCAACTGATCCATATCATGTACACTGTTATATTCCATGAGATTTTACTTAAAT ATTCATTTATATGGCTAATTATAGCTGGATTATAATATTTGTCCTTTCAAATATAAGACA GCATTTTTGCCCCCCCACTGCAAAAATGTGCATTATACCTTGCAGATGAAATTCTGAGAC TGAGTTGCCTTTTTGTTTTATATCATTTGAGCTTATGATGTCAGAAATACCAAAATAGAA ACATGAAAATCAAAAGTGGCAAATGTGTGAAGCATTTAGTAATAAAAATAGGTGGCAGAC CTCATGTTTGTTTCTGAAATGTTTTAACGGATGACAGTGAGAATAGATACTATAAATTAA TTCCTCTCTTTAGCGATTGTTACAATAGATACTATAAATTAATTCCTCTCTTTAGCAGTT GTTACAGTATATACTATAATTCCTCTTTTTAGCGATTGTTATCTAGCTGTTCTAAACCTG TGTTTTACGAATTTGATTACTTCTTTGTTTAAATGTATTATCTTTCATATGGCTCTAAGT TGTTTTGGAAATCAATTCAAATAAACTTTAATATTAAACCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009775 unedited NCCGTTAGGATTTGTAATACGACTCACTATAGGGGCGGCCGCGGAATTCGCCATTACGGC CGGGGTGGACCAGCCGTGCAAATCTCTAGAAGATGACGGTGTTCTTTAAAACGCTTCGAA ATCACTGGGAAGAAAACTACAGCTGGGCTCTGCCTGCTGACCTGGGGAGGCCATTGGCTC TATGGAAAACACTGTGATAACCTCCTAAGGAGAGCAGCCTGTCAAGAAGCTCAGCACTAT CAGGATGAATCACGCTGGGAGCCAACTCTGAGTAGAACTCCAGGAAGTTAACCATCTGGT GAAGAGCAGTTCTGACGCCCACCCCTATAGACTGTTTGTTTCTGGATTGGTCCCAAGAAC ACAGAACTGGACTGGTAGAAGCTCTGGGAATTTGTTTGTGTCTGCAGTTGTCTTTGTAGA TTTGTCTAACCCCTGAATCATCCCTGATCTGAATAACCCCCAAGGATATCAGTTCAGAGC AAGTGTTACCTCTAAAAGTCAAATCTTCTTAAAATTGTTGATTCATATTTTTAAAGCTCA TCTCCAAGTTCAACTGATCCATATCATGTACACTGTTATATTCCATGAGATTTTACTTAA ATATTCATTTATATGGCTAATTATAGCTGGATTATAATATTTGTCCTTTCAAATATAAGA CAGCATTTTTGCCCCCCCACTGCAAAAATGTGCATTATACCTTGCAGATGAAATTCTGAG ACTGAGTTGCCTTTTTGTTTTATATCATTTGAGCTTATGATGTCAGAGATACCCAAATAG AAACATGAAAATCANAAGTGGCANATGTGTGAAGCATTTAGTAATAAAAATAGGTGGCAG ACCTCATGTGTGTTTCTGAGATGTTTTAACGGATGACAGTGAGAATAGATACTATNAATT TAATTCCCTCTCTTAGCGAG
Restriction Sites	Please inquire
ACCN	BC009775
Insert Size	1090 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009775.1, AAH09775.1
RefSeq Size	1090 bp
Locus ID	55750
Protein Pathways	Glycerolipid metabolism, Metabolic pathways
Gene Summary	The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]

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