Aurora B (AURKB) (BC009751) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AIK2; AIM-1; AIM1; ARK2; AurB; aurora-1; aurora-B; aurora kinase B; IPL1; serine/threonine kinase 12; STK5; STK12
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009751 edited GTGCCTTGGACCCCAGCTCTCCTCCCCCTTTCTCTCTAAGGATGGCCCAGAAGGAGAACT CCTACCCCTGGCCCTACGGCCGACAGACGGCTCCATCTGGCCTGAGCACCCTGCCCCAGC GAGTCCTCCGGAAAGAGCCTGTCACCCCATCTGCACTTGTCCTCATGAGCCGCTCCAATG TCCAGCCCACAGCTGCCCCTGGCCAGAAGGTGATGGAGAATAGCAGTGGGACACCCGACA TCTTAACGCGGCACTTCACAATTGATGACTTTGAGATTGGGCGTCCTCTGGGCAAAGGCA AGTTTGGAAACGTGTACTTGGCTCGGGAGAAGAAAAGCCATTTCATCGTGGCGCTCAAGG TCCTCTTCAAGTCCCAGATAGAGAAGGAGGGCGTGGAGCATCAGCTGCGCAGAGAGATCG AAATCCAGGCCCACCTGCACCATCCCAACATCCTGCGTCTCTACAACTATTTTTATGACC GGAGGAGGATCTACTTGATTCTAGAGTATGCCCCCCGCGGGGAGCTCTACAAGGAGCTGC AGAAGAGCTGCACATTTGACGAGCAGCGAACAGCCACGATCATGGAGGAGTTGGCAGATG CTCTAATGTACTGCCATGGGAAGAAGGTGATTCACAGAGACATAAAGCCAGAAAATCTGC TCTTAGGGCTCAAGGGAGAGCTGAAGATTGCTGACTTCGGCTGGTCTGTGCATGCGCCCT CCCTGAGGAGGAAGACAATGTGTGGCACCCTGGACTACCTGCCCCCAGAGATGATTGAGG GGCGCATGCACAATGAGAAGGTGGATCTGTGGTGCATTGGAGTGCTTTGCTATGAGCTGC TGGTGGGGAACCCACCCTTTGAGAGTGCATCACACAACGAGACCTATCGCCGCATCGTCA AGGTGGACCTAAAGTTCCCCGCTTCCGTGCCCATGGGAGCCCAGGACCTCATCTCCAAAC TGCTCAGGCATAACCCCTCGGAACGGCTGCCCCTGGCCCAGGTCTCAGCCCACCCTTGGG TCCGGGCCAACTCTCGGAGGGTGCTGCCTCCCTCTGCCCTTCAATCTGTCGCCTGATGGT CCCTGTCATTCACTCGGGTGCGTGTGTTTGTATGTCTGTGTATGTATAGGGGAAAGAAGG GATCCCTAACTGTTCCCTTATCTGTTTTCTACCTCCTCCTTTGTTTAATAAAGGCTGAAG CTTTTTGTACTCATGAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009751 unedited NCGTTCAGAATTGTAAACGACTTCACTATAGGCGGCCGCGAATTCGCACGAGGAGGGTCG GCCGGGNAAAGTAGCAGTGCCTTGGACCCCAGCTCTCCTCCCCCTTTCTCTCTAAGATGG CCCAGAAGGAGAACTCCTACCCCTGGCCCTACGGCCGACAGACGGCTCCATCTGGCCTGA GCACCCTGCCCCAGCGAGTCCTCCGGAAAGAGCCTGTCACCCCATCTGCACTTGTCCTCA TGAGCCGCTCCAATGTCCAGCCCACAGCTGCCCCTGGCCAGAAGGTGATGGAGAATAGCA GTGGGACACCCGACATCTTAACGCGGCACTTCACAATTGATGACTTTGAGATTGGGCGTC CTCTGGGCAAAGGCAAGTTTGGAAACGTGTACTTGGCTCGGGAGAAGAAAAGCCATTTCA TCGTGGCGCTCAAGGTCCTCTTCAAGTCCCAGATAGAGAAGGAGGGCGTGGAGCATCAGC TGCGCAGAGAGATCGAAATCCAGGCCCACCTGCACCATCCCAACATCCTGCGTCTCTACA ACTATTTTTATGACCGGAGGAGGATCTACTTGATTCTAGAGTATGCCCCCCGCGGGGAGC TCTACAAGGAGCTGCAGAAGAGCTGCACATTTGACGAGCAGCGAACAGCCACGATCATGG AGGAGTTGGCAGATGCTCTAATGTACTGCCATGGGAAGAAGGTGATTCACAGAGACATAA AGCCAGAAAATCTGCTCTTAGGGCTCAAGGGAGAGCTGAAGATTGCTGACTTCGGCTGGG TCTGTGCATGCGCCCTCCCTNGAGAGGAAGACAATGTGTGGCACCCTGGACTACCTGCCC CCAGAATGATTGGAGGGCGCATGCACAATGAGAAGTGGATCTGTTGTGCATTGGAGTGCT TTGCTATGAGCTGCTGGTGGGGGAACCCACCTTTTGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC009751 unedited NTTGCGCCATTGGCTGATGGCAACTTGCCAGGTCCAGGAAGAGCCTGGGGAAGGGGCTCA CAGGGCATGCCACCCGGGTATCTGTTGAGGAAAACAGCTATGACCGCGGCCGCAATCTAG AGTCGAGTTTTTTTTTTTTTTTTTTACAAAAAGCTTCAGCCTTTATTAAACAAAGGAGGA GGTAGAAAACAGATAAGGGAACAGTTAGGGATCCCTTCTTTCCCCTATACATACACAGAC ATACAAACACACGCACCCGAGTGAATGACAGGGACCATCAGGCGACAGATTGAAGGGCAG AGGGAGGCAGCACCCTCCGAGAGTTGGCCCGGACCCAAGGGTGGGCTGAGACCTGAGCCA GGGGCAGCCGTTCCGAGGGGTTATGCCTGATCAGTTATGAGATGATGTCCTGCGCTCCCG TGGGCACAGAAGCGGGGAACTTTAGGTCCACCTTGACGATGCGGCGATAGGTCTCGTTGT GTGATGCACTCTCAAAGGGTGGGGTTCCCCACCAGCAGCTCATAGCANAGCACTCCAATG CACCACAGATCCACCTTCTCATTGTGCATGCGCCCCTCAATCATCTCTGGGGGGCATGTA GTCCAGGGTGCCACACATTGTCTTCCTCCTCAGGGAAGGCGCATGCACAGACCAGCGAAG TCAGCANTCTTCAGCTCTCCTTTGACCCTAAGACAAATTTTCTAGCCTTATGTCTCTGTA AATCACCTCTTCCCATGGCAGTACATAANAGCATCTGCCACCTCCTCCATGATCCGGCCT TGTCCCTGCTAGTAAATGTGCAGCTCTTCTGCAGCTCTTGTAAACTCCCCGCGGGGGCAT ACTCTAAATCAAGTACATCTCCTCCGTCATAAAATANTTTAAAACCCAGATGTTGGATGG TCCCAGTGGCCCGGGATTTCATTCTTTTTGCCCACTGAAGT
Restriction Sites	Please inquire
ACCN	BC009751
Insert Size	1242 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009751.2, AAH09751.1
RefSeq Size	1233 bp
Locus ID	9212
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Gene Summary	This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]

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