ACK1 (TNK2) (BC008884) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACK; ACK-1; ACK1; p21cdc42Hs
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC008884 edited GGCACGAGGGGGATTTGGGGGGCTCCTGGGCAGAGTGCGAAGCTCCGGAGCCGAGGCCAC TGGATCCCCAGTGACTGCTCGGGGGGGTCCCGCTGCGTGGAGATGCCGGCAGCACGTCGG TTCCCTGGCCTGGAGCTCTCCTTCCCTCTCCTGGCCAGACTGCGGCGACGCCTGTACACA AGGCTGGGAGGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTG TCCGAGGTGCAGCTGCAACAGTACTTCCTGCGGCTCCGAGATGACCTCAACGTCACCCGC CTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCCT GGCCAGCGGCGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCG TGGATGAGTAAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTTCCCACCTCATCACTCT CAGAGCACCTTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTG CAGAGCCTCACCTGCCTCATTGGGGAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGAT GGTTCCTTTGTCGTGGTGCGCAGGGGCGAGTGGGACGCGCCCTCAGGGAAGACGGTGAGT GTGGCTGTGAAGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCATGGACGACTTC ATCCGGGAGGTCAATGCCATGCACTCGCTCGACCACCGAAACCTCATCCGCCTCTACGGG GTGGTGCTCACGCCGCCCATGAAGATGGTGACAGAGCTGGCACCTCTGGGATCGTTGTTG GACCGGCTACGTAAGCACCAGGGCCACTTCCTCCTGGGGACTCTGAGCCGCTACGCTGTG CAGGTGGCTGAGGGCATGGGCTACCTGGAGTCCAAGCGCTTTATTCACCGTGACCTGGCT GCCCGCAATCTGCTGTTGGCTACCCGCGACCTGGTCAAGATCGGGGACTTTGGGCTGATG CGAGCACTACCTCAGAATGACGACCATTACGTCATGCAGGAACATCGCAAGGTGCCCTTC GCCTGGTGTGCCCCCGAGAGCCTGAAGCCGCCCTGGAGGGACATCAGTGCTTCCTCTTCC ACCCAATTCCCCCACGCGGTTCCATGTTTTCCCACCAGCCTGTTGGCGAAGTTGCTGCTC CGGCATTCAGTACCTGCTTCTTCCAGAGAAATAAAGTTAGTTTCTATTTTATGTTAAAAA AAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008884 unedited NNNNNNNGAGTCAACATTTGTNAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGG GGGATTTGGGGGGCTCCTGGGCAGAGTGCGAAGCTCCGGAGCCGAGGCCACTGGATCCCC AGTGACTGCTCGGGGGGGTCCCGCTGCGTGGAGATGCCGGCAGCACGTCGGTTCCCTGGC CTGGAGCTCTCCTTCCCTCTCCTGGCCAGACTGCGGCGACGCCTGTACACAAGGCTGGGA GGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTGTCCGAGGTG CAGCTGCAACAGTACTTCCTGCGGCTCCGAGATGACCTCAACGTCACCCGCCTGTCCCAC TTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCCTGGCCAGCGG CGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCGTGGATGAGT AAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTTCCCACCTCATCACTCTCAGAGCACC TTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTGCAGAGCCTC ACCTGCCTCATTGGGGAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGATGGTTCCTTT GTCGTGGTGCGCATGGGCGAGTGGGACGCGCCCTCAGGGAAGACGGTGAGTGTGGCTGTG AAGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCATGGACGACTTCATCCGGGAG GTCAATGCCATGCACTCGCTCGACCACCGAAACCTCATCCGCCTCTACGGAGTGGTGCTC ACGCCGCCCAT
Restriction Sites	Please inquire
ACCN	BC008884
Insert Size	1275 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008884.1, AAH08884.1
RefSeq Size	1275 bp
Locus ID	10188
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

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