MDFI (BC007836) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	I-MF; I-mfa; inhibitor of MyoD family a; MyoD family inhibitor; OTTHUMP00000016375; OTTHUMP00000196414
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC007836 edited GAGAAAGCAGCGAGTGAGAGGGGAAGGGGCGCCAGGCGAGCACCCGGGAGCCAGCGGGAC CTGGGCAGGGGCGCCCGGAGCAGGCGCGCATGGCGGGCCCCGCGCGGGGATCCGGCTGGA AGAGAGCGTAGCACGGCTCGCACGAGTCCGGGGCCGATGTACCAGGTGAGCGGCCAGCGC CCCTCTGGCTGCGACGCGCCCTATGGAGCCCCCAGCGCAGCCCCGGGCCCAGCCCAGACC CTATCCCTCCTTCCTGGGCTGGAGGTAGTAACAGGATCCACTCACCCTGCGGAGGCAGCA CCAGAGGAGGGCTCCCTGGAGGAGGCGGCAACCCCCATGCCCCAAGGCAATGGCCCTGGC ATCCCCCAGGGCCTGGACAGCACTGACCTCGACGTCCCCACAGAAGCTGTGACATGCCAG CCTCAGGGGAACCCCTTGGGCTGCACCCCACTTCTGCCGAATGACTCTGGCCACCCCTCA GAGCTGGGCGGCACCAGACGGGCGGGGAATGGTGCCCTGGGTGGCCCCAAGGCCCACCGG AAGTTGCAGACACACCCATCTCTCGCCAGCCAGGGCAGCAAGAAGAGTAAGAGCAGCAGC AAATCCACCACCTCCCAGATCCCCCTCCAGGCACAGGAAGACTGCTGTGTCCACTGCATC CTGTCCTGCCTGTTCTGCGAGTTCCTGACGCTGTGCAACATCGTCCTGGACTGCGCCACC TGTGGCTCCTGCAGCTCGGAGGACTCGTGCCTCTGCTGCTGCTGCTGTGGCTCTGGCGAG TGTGCCGACTGCGACCTGCCCTGCGACCTGGACTGCGGCATCCTGGATGCCTGCTGCGAG TCCGCGGACTGCCTGGAGATCTGCATGGAGTGCTGTGGGCTCTGCTTCTCCTCCTGAGCC TCTGTCGGGGGCTAAGCCAGCCTGGCGCCCCTGCAGATTCCAGCAGGGTCCCTCTGAGTG GGGCCAGGCCCAGGACTGTCACACAAGGCTTGAGAAGCCCCCTCTCCCTGGTCCTCTCCT ACCCACCCATGTCCTCTCAGAACCCCAGCCTTGAAAATAGTGGGGGGCACTCAGAGGGGC CACCTCCTCAGCCGTGGGTGGTGGGCCCATGGCAGAGAAGCCTGAACTCTTTACTGGGTT ACCAGGTTCATACATTGCTGAGGACCTGACAGGACAACCTAGGGGCAGGGCTGGGGTGGG GACCGCAGGGGGCAGCCAGGGCTGGGGAACACTGTGAAAGTTACTTGGGGAGGGTGGGCC GGTGGGGCCGTAGCTCTCTACCTCTCCCTGCTCCTGGTGCCTGCCTCTCTCCTCCACCCC AGGCTTAGAGGACAGAAAAATGTGAAGAGACGCCCCACCCACCCTCAGCCAGCCCTCTCC AGTCTCCTTTCCTAGGCTTTTTTGGGGGCCTAACCCACGCAGTGACCCCAGAGGGCAGGG CTAGGCGAGAGCCTGGGGTGGGGCGGGAGGGGGAACAGTATGGAAAAGACTGGAAGGGGA AAGGAAGGGAAGGGAGGGAGGGTCTGTTCTATCTGTTGCTGTAAATAAAGATATTTGTCC ATCTCAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC007836 unedited GGCGCCAGAATTGTATACGACTTATATAGGCGGCACGCGAATTCGCACGAGGAGAAAGCG CGAGTGAGAGGGGAAGGGGCGCCAGGCGAGCACCCGGGAGCCAGCGGGACCTGGGCAGGG GCGCCCGGAGCAGGCGCGCATGGCGGGCCCCGCGCGGGGATCCGGCTGGAAGAGAGCGTA GCACGGCTCGCACGAGTCCGGGGCCGATGTACCAGGTGAGCGGCCAGCGCCCCTCTGGCT GCGACGCGCCCTATGGAGCCCCCAGCGCAGCCCCGGGCCCAGCCCAGACCCTATCCCTCC TTCCTGGGCTGGAGGTAGTAACAGGATCCACTCACCCTGCGGAGGCAGCACCAGAGGAGG GCTCCCTGGAGGAGGCGGCAACCCCCATGCCCCAAGGCAATGGCCCTGGCATCCCCCAGG GCCTGGACAGCACTGACCTCGACGTCCCCACAGAAGCTGTGACATGCCAGCCTCAGGGGA ACCCCTTGGGCTGCACCCCACTTCTGCCGAATGACTCTGGCCACCCCTCAGAGCTGGGCG GCACCAGACGGGCGGGGAATGGTGCCCTGGGTGGCCCCAAGGCCCACCGGAAGTTGCAGA CACACCCATCTCTCGCCAGCCAGGGCAGCAAGAAGAGTAAGAGCAGCAGCAAATCCACCA CCTCCCAGATCCCCCTCCAGGCACAGGAAGACTGCTGTGTCCACTGCATCCTGTCCTGCC TGTTCTGCGAGTTCCTGACGCTGTGCAACATCGTCCTGGACTGCGCCACCTGTGGCTCCT GCAGCTCGGAGGACTCGTGCCTCTGCTGCTGCTGCTGTGGCTCTGGCGAGTGTGCCGACT GCGACCTGCCCTGCGACCTGGACTGCGGCATCCTGGATGCCTGCTGCGAGTCCGCGGCT
Restriction Sites	Please inquire
ACCN	BC007836
Insert Size	1592 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC007836.2, AAH07836.1
RefSeq Size	1583 bp
Locus ID	4188
Protein Families	Transcription Factors
Gene Summary	This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008]

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