RANTES (CCL5) (BC003528) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC003528 edited CTGACTTGGACCCTTCTTTGGAGAGGAGCAGGAAGGACCTCCAAGGTGAAGGGGTGAGGG GATGCCAGGTTTCTGAGCAGTGGGCTGGCCCTGGCATAATGCTATGGTGTTGAACGACCA CAGCTCACAGGTCCCTCATGGAGGCCTGAGCCCTTCAATGCCAGGACTTGGACCTGCCAT TCTGGTCAGCGCCACGGCTGTGGACACAGCCATCTGGTCCCCCCAACGGGATGGAGCAGG CCTGCCATCTAGATCCTGCCAGGGGCCGAGGAGGCGAGAGTTGCTAGGGAAGTGATTAGT TTACCCTTAAGTAAGTACCTGAGCCTGTTTCAGTGATCAATAAAATCAGCAATGTTAGCC GGGAATGGTGGCTTATGACTGTAATCCCAGCATTTCAGGAGGCTGAAGCCGGAGGATCGC TTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACATGGTGAAACCCTGTCTCTACTAAAA TATACAAAAATTAGCCAGGTGAGGTGGCATGTGCCTGTAGTCCCAGGTACTCAGGAGGCT GAGGTAGGAGGATCACTGATGTATGTGTGTATACACACACACATGTATTTGTAGATTGGG GGTCCCCAAGACCACCCTTAGGTTTGACACCTTGCTAGAAGAACTCAGAGAATGTATTAA AAACTATTATGCTCATGGTCATGGTTTATTACAGCAAAAAGATACAGATTAGGATGGGTG CGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGAGGGAGGATCACTTGAG GTCAGGAGTTCGAGACCAGCCTGACCAACATGATCAAACCCCAACTCTACTAAAAACCCA AAAATTAGCCGGGCATAGTGGTGGGCGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCA GAAGAATCGCTTGAACCCAGGAGGCACAGATTGCAATGAGCCTAGATTGTGCCTCTGTAC TCCAGCCTGGATGACAGAGTGAGACTCAGTCTCAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC003528 unedited NNNCCGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGAC TTGGACCCTTCTTTGGAGAGGAGCAGGAAGGACCTCCAAGGTGAAGGGGTGAGGGGATGC CAGGTTTCTGAGCAGTGGGCTGGCCCTGGCATAATGCTATGGTGTTGAACGACCACAGCT CACAGGTCCCTCATGGAGGCCTGAGCCCTTCAATGCCAGGACTTGGACCTGCCATTCTGG TCAGCGCCACGGCTGTGGACACAGCCATCTGGTCCCCCCAACGGGATGGAGCAGGCCTGC CATCTAGATCCTGCCAGGGGCCGAGGAGGCGAGAGTTGCTAGGGAAGTGATTAGTTTACC CTTAAGTAAGTACCTGAGCCTGTTTCAGTGATCAATAAAATCAGCAATGTTAGCCGGGAA TGGTGGCTTATGACTGTAATCCCAGCATTTCAGGAGGCTGAAGCCGGAGGATCGCTTGAG CCCAGGAGTTTGAGACCAGCCTGGGCAACATGGTGAAACCCTGTCTCTACTAAAATATAC AAAAATTAGCCAGGTGAGGTGGCATGTGCCTGTAGTCCCAGGTACTCAGGAGGCTGAGGT AGGAGGATCACTGATGTATGTGTGTATACACACACACATGTATTTGTAGATTGGGGGTCC CCAAGACCACCCTTAGGTTTGACACCTTGCTAGAAGAACTCAGAGAATGTATTAAAAACT ATTATGCTCATGGTCATGGTTTATTACAGCANANAGATACAGATTANNGATGGGTGCGGG CTATGCCCCGGTGGAATCCCANCACTTTGGGAGGCTGAGGAGGGGAGGATCACTTGAGTC AGNAGNTTCCNGGAACCACCCTGAACAACCTGATTCAAACCCACCTCTACTAAAAACCCC AAAA
Restriction Sites	Please inquire
ACCN	BC003528
Insert Size	1018 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003528.1
RefSeq Size	1018 bp
Locus ID	6352
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway
Gene Summary	This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

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