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LIM Kinase 1 (LIMK1) (NM_002314) Human Untagged Clone
CAT＃: SC123883
LIMK1 (untagged)-Human LIM domain kinase 1 (LIMK1), transcript variant 1
CNY 4,824.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LIMK; LIMK-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002314 edited
GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAAT TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGCCTCGGTCCG CCCCTCCGCTCGCTCCCCAAGCCGCCGCGGCGCCGAGCCGGTTTCCCCGCCGGTGTCCGA GAGGCGCCCCCGGCCCGGCCGCCCCCAGCCCCAGCCCCGCCGGGCCCCGCCCCCCGTCGA GTGCATGAGGTTGACGCTACTTTGTTGCACCTGGAGGGAAGAACGTATGGGAGAGGAAGG AAGCGAGTTGCCCGTGTGTGCAAGCTGCGGCCAGAGGATCTATGATGGCCAGTACCTCCA GGCCCTGAACGCGGACTGGCACGCAGACTGCTTCAGGTGTTGTGACTGCAGTGCCTCCCT GTCGCACCAGTACTATGAGAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCG CTATGGCGAGTCCTGCCATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTGGC TGGGGAGCTGAAGTACCACCCCGAGTGTTTCATCTGCCTCACGTGTGGGACCTTTATCGG TGACGGGGACACCTACACGCTGGTGGAGCACTCCAAGCTGTACTGCGGGCACTGCTACTA CCAGACTGTGGTGACCCCCGTCATCGAGCAGATCCTGCCTGACTCCCCTGGCTCCCACCT GCCCCACACCGTCACCCTGGTGTCCATCCCAGCCTCATCTCATGGCAAGCGTGGACTTTC AGTCTCCATTGACCCCCCGCACGGCCCACCGGGCTGTGGCACCGAGCACTCACACACCGT CCGCGTCCAGGGAGTGGATCCGGGCTGCATGAGCCCAGATGTGAAGAATTCCATCCACGT CGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCCGAAATGTGCCCCTGGACGAGAT TGACCTGCTGATTCAGGAAACCAGCCGCCTGCTCCAGCTGACCCTCGAGCATGACCCTCA CGATACACTGGGCCACGGGCTGGGGCCTGAGACCAGCCCCCTGAGCTCTCCGGCTTATAC TCCCAGCGGGGAGGCGGGCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGCAT CGACAGGTCTCCGGGCGCTGGCTCACTGGGCTCCCCGGCCTCCCAGCGCAAGGACCTGGG TCGCTCTGAGTCCCTCCGCGTAGTCTGCCGGCCACACCGCATCTTCCGGCCGTCGGACCT CATCCACGGGGAGGTGCTGGGCAAGGGCTGCTTCGGCCAGGCTATCAAGGTGACACACCG TGAGACAGGTGAGGTGATGGTGATGAAGGAGCTGATCCGGTTCGACGAGGAGACCCAGAG GACGTTCCTCAAGGAGGTGAAGGTCATGCGATGCCTGGAACACCCCAACGTGCTCAAGTT CATCGGGGTGCTCTACAAGGACAAGAGGCTCAACTTCATCACTGAGTACATCAAGGGCGG CACGCTCCGGGGCATCATCAAGAGCATGGACAGCCAGTACCCATGGAGCCAGAGAGTGAG CTTTGCCAAGGACATCGCATCAGGGATGGCCTACCTCCACTCCATGAACATCATCCACCG AGACCTCAACTCCCACAACTGCCTGGTCCGCGAGAACAAGAATGTGGTGGTGGCTGACTT CGGGCTGGCGCGTCTCATGGTGGACGAGAAGACTCAGCCTGAGGGCCTGCGGAGCCTCAA GAAGCCAGACCGCAAGAAGCGCTACACCGTGGTGGGCAACCCCTACTGGATGGCACCTGA GATGATCAACGGCCGCAGCTATGATGAGAAGGTGGATGTGTTCTCCTTTGGGATCGTCCT GTGCGAGATCATCGGGCGGGTGAACGCAGACCCTGACTACCTGCCCCGCACCATGGACTT TGGCCTCAACGTGCGAGGATTCCTGGACCGCTACTGCCCCCCAAACTGCCCCCCGAGCTT CTTTCCCATCACCGTGCGCTGTTGCGATCTGGACCCCGAGAAGAGGCCATCCTTTGTGAA GCTGGAACACTGGCTGGAGACCCTCCGCATGCACCTGGCCGGCCACCTGCCACTGGGCCC ACAGCTGGAGCAGCTGGACAGAGGTTTCTGGGAGACCTACCGGCGCGGCGAGAGCGGACT GCCTGCCCACCCTGAGGTCCCCGACTGAGCCAGGGCCACTCAGCTGCCCCTGTCCCCACC TCTGGAGAATCCACCCCCACCAGATTCCTCCGCGGGAGGTGGCCCTCAGCTGGGACAGTG GGGACCCAGGCTTCTCCTCAGAGCCAGGCCCTGACTTGCCTTCTCCCACCCCGTGGACCG CTTCCCCTGCCTTCTCTCTGCCGTGGCCCAGAGCCGGCCCAGCTGCACACACACACCATG CTCTCGCCCTGCTGTAACCTCTGTCTTGGCAGGGCTGTCCCCTCTTGCTTCTCCTTGCAT GAGCTGGAGGGCCTGTGTGAGTTACGCCCCTTTCCACACGCCGCTGCCCCAGCAACCCTG TTCACGCTCCACCTGTCTGGTCCATAGCTCCCTGGAGGCTGGGCCAGGAGGCAGCCTCCG AACCATGCCCCATATAACGCTTGGGTGCGTGGGAGGGCGCACATCAGGGCAGAGGCCAAG TTCCAGGTGTCTGTGTTCCCAGGAACCAAATGGGGAGTCTGGGGCCCGTTTTCCCCCCAG GGGGTGTCTAGGTAGCAACAGGTATCGAGGACTCTCCAAACCCCCAAAGCAGAGAGAGGG CTGATCCCATGGGGCGGAGGTCCCCAGTGGCTGAGCAAACAGCCCCTTCTCTCGCTTTGG GTCTTTTTTTTGTTTCTTTCTTAAAGCCACTTTAGTGAGAAGCAGGTACCAAGCCTCAGG GTGAAGGGGGTCCCTTGAGGGAGCGTGGAGCTGCGGTGCCCTGGCCGGCGATGGGGAGGA GCCGGCTCCGGCAGTGAGAGGATAGGCACAGTGGACCGGGCAGGTGTCCACCAGCAGCTC AGCCCCTGCAGTCATCTCAGAGCCCCTTCCCGGGCCTCTCCCCCAAGGCTCCCTGCCCCT CCTCATGCCCCTCTGTCCTCTGCGTTTTTTCTGTGTAATCTATTTTTTAAGAAGAGTTTG TATTATTTTTTCATACGGCTGCAGCAGCAGCTGCCAGGGGCTTGGGATTTTATTTTTGTG GCGGGCGGGGGTGGGAGGGCCATTTTGTCACTTTGCCTCAGTTGAGCATCTAGGAAGTAT TAAAACTGTGAAGCTTTCTCAGTGCACTTTGAACCTGGAAAACAATCCCAACAGGCCCGT GGGACCATGACTTAGGGAGGTGGGACCCACCCACCCCCATCCAGGAACCGTGACGTCCAA GGAACCAAACCCAGACGCAGAACAATAAAATAAATTCCGTACTCCCCACCCAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA >OriGene 5' read for NM_002314 unedited
ATAGGGCGGCCGCGAATTCGGCACGAGGCCCGCCTCGGTCCGCCCCTCCGCTCGCTCCCC AAGCTCGCCGCGGCGCCGAGCCGGTTTCCCCGCCGGTGTCCGAGAGGCGCCCCCGGCCCG GCCGCCCCCAGCCCCAGCCCCGCCGGGCCCCGCCCCCCGTCGAGTGCATGAGGTTGACGC TACTTTGTTGCACCTGGAGGGAAGAACGTATGGGAGAGGAAGGAAGCGAGTTGCCCGTGT GTGCAAGCTGCGGCCAGAGGATCTATGATGGCCAGTACCTCCAGGCCCTGAACGCGGACT GGCACGCAGACTGCTTCAGGTGTTGTGACTGCAGTGCCTCCCTGTCGCACCAGTACTATG AGAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCGCTATGGCGAGTCCTGCC ATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTGGCTGGGGAGCTGAAGTACC ACCCCGAGTGTTTCATCTGCCTCACGTGTGGGACCTTTATCGGTGACGGGGACACCTACA CGCTGGTGGAGCACTCCAAGCTGTACTGCGGGCACTGCTACTACCAGACTGTGGTGACCC CCGTCATCGAGCAGATCCTGCCTGACTCCCCTGGCTCCCAGCTGCCCCACACCGTCACCC TGGTGTCCATCCCAGCCTCATCTCATGGCAAGCGTGGACTTTCAGTCTCCATTGACCCCC CGCACGGCCCACCGGGCTGTGGN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002314 |
| Insert Size | 3500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002314.2, NP_002305.1 |
| RefSeq Size | 3332 bp |
| RefSeq ORF | 1944 bp |
| Locus ID | 3984 |
| UniProt ID | P53667 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The exon structure of the 5' UTR has been inferred from cross-species comparisons and lacks direct experimental support. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218058 | LIMK1 (Myc-DDK-tagged)-Human LIM domain kinase 1 (LIMK1), transcript variant 1 |
CNY 4,816.00 |
|
| RC218058L1 | Lenti ORF clone of Human LIM domain kinase 1 (LIMK1), transcript variant 1, Myc-DDK-tagged |
CNY 7,216.00 |
|
| RC218058L2 | Lenti ORF clone of Human LIM domain kinase 1 (LIMK1), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| RC218058L3 | Lenti ORF clone of Human LIM domain kinase 1 (LIMK1), transcript variant 1, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC218058L4 | Lenti ORF clone of Human LIM domain kinase 1 (LIMK1), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| RG218058 | LIMK1 (tGFP-tagged) - Human LIM domain kinase 1 (LIMK1), transcript variant 1 |
CNY 6,416.00 |
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