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CARS1 (NM_139273) Human Untagged Clone
CAT＃: SC124342
CARS (untagged)-Human cysteinyl-tRNA synthetase (CARS), transcript variant 1
CNY 5,320.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CARS; CYSRS; MCDDBH; MDBH; MGC:11246 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124342 sequence for NM_139273 edited (data generated by NextGen Sequencing)
ATGGCAGATTCCTCCGGGCAGCAGGGCAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCT CCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAA GTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTAT GACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTTTGATATCTTGAGAAGAGTG TTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGAC AAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCT GAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTA AATGAGACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAG CTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGAGGAAGTCAAC AGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCT ACACTTGGCTGTGATGTCACTGACAATTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAG GGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGG GTTAGTGAGTATGTGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGTTAC GGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTCTAGCGAGAAG CACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGG GAAGGTGACCTGAGCATCTCTGCAGACCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTT GCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGT CGTCCGGGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATG GACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCATGACAATGAGCTGGCACAG TCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTG ACCATTGCAGGCTGCAAAATGTCAAAGTCACTAAAAAACTTCATCACCATTAAAGATGCC TTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGAC ACCCTGGACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAAT GAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCACTGGTCAGTTT GAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACAGCA ATTCACAAAGCCCTCTGTGACAATGTTGACACCCGCACCGTCATGGAAGAGATGCGGGCC TTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAAC CAGGCTCTGCTGGAGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGGGCC GTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAGCCTCAGTCTC GAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAG ATTGCCCGAGAGCAAAAAGTCCCTGAGATTCTGCAGCTCAGCGATGCCCTGCGGGACAAC ATCCTGCCCGAGCTTGGGGTGCGGTTTGAAGACCACGAAGGACTGCCCACAGTGGTGAAA CTGGTAGACAGAAACACCTTATTAAAAGAGAGAGAAGAAAAGAGACGGGTTGAAGAGGAG AAGAGGAAGAAGAAAGAGGAGGCGGCCCGGAGGAAACAGGAACAAGAAGCAGCAAAGCTG GCCAAGATGAAGATTCCCCCCAGTGAGATGTTCTTGTCAGAAACCGACAAATACTCCAAG TTTGATGAAAATGTAAGCATGGTCTGCCCACACATGACATGGAGGGCAAAGAGCTCAGCA AAGGGCAAGCCAAGAAGCTGA Clone variation with respect to NM_139273.3 >OriGene 5' read for NM_139273 unedited
AGGAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGTAGCCGCAG CTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGGCAAAGGCCGGC GTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACA ACAGCCTCACCAGGAACAAGGAAGTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGT ATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCT CTTTTGATATCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCA TGAACATTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCG AGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCC TGAAGCCATTTTCAGTAAAATTAAATGAGACCACGGATCCCGATAAAAAGCAGATGCTCG AACGGATTCAGCACGCAGTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCA GACTCACGGGAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATT TGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCCATCTTCT CCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAAATGTT CTCCTCCAGATGTCTTAACCCGGGTTAGTGAGTATGTGCCAGAAATTGTGAACTTGTCCA GAAGAATGTGACACGGTTACGCTATGTCTCCAATGGTCTGTCTACTTGAACAGCGAAGTT GCTTCTACGAGAGCACTCTATGAAGNCTGTGCTGAGGCGTTGNAATCAAAAGCCTCAGAG GGGAGTGACTGACATTT >OriGene 3' read for NM_139273 unedited
TTTTTTTCGTTTACTAAGAACCGCGGCCGCATACNANGATCGNATTTTTTTTTTTTTTTT TTCACTGTTNGAAAAAAATTATTATCAATGTCTCAAAGCCAACGACGACACGAACCTACA TGAACACAACTCTTAATTTAGGACCCAAGGGTGACTGTAAACATGATAGGAGCGCTGGGA CATTGTCACTGAGGCAGACAGCAGCCACTAGTCCACAATGGTTTAAAAAGTCAGTCCTGT GCCCCCTCACTGGAAGCTTCCATTCTGGGCCATCTGCAGATATTCCTTGTAGAGCTTCTC CTGAGCCTCGAAGAGCTTCTTCAGCTTCTTGGCTTGCCCTTTGCTGAGCTCTTTGCCCTC CATGTCATGTGTGGGCAGACCATGCTTACATTTTCATCAAACTTGGAGTATTTGTCGGTT TCTGACAAGAACATCTCACTGGGGGGAATCTTCATCTTGGCCAGCTTTGCTGCTTCTTGT TCCTGTTTCCTCCGGGCCGCCTCCTCTTTCTTCTTCCTCTTCTCCTCTTCAACCCGTCTC TTTTCTTCTCTCTCTTTTAATAAGGTGTTTCTGTCTACCAGTTTCACCACTGTGGGCAGT CCTTCGTGGTCTTCAAACCGCACCCCAAGCTCGGGCAGGATGTTGTCCCGCAGGGCATCG CTGAGCTGCAGAATCTCAGGGACTTTTTGCTCTCGGGCAATCTTCCGCACTCCTTCTCGG AATTCTGATAACACCNTGAGGTAGGGCATGACTGTGGCCTCGAGACTGAGGCTGGTTCCA GGCCCTCCGACCGGGAATCCCAGGGAGCTGTCCTCTTCTACGGCCCCAAAGTCTTCACAT ATGGGTGAAGTACAGGGCGATGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_139273 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139273.2, NP_644802.1 |
| RefSeq Size | 2536 bp |
| RefSeq ORF | 2181 bp |
| Locus ID | 833 |
| UniProt ID | P49589 |
| Domains | tRNA-synt_1e |
| Protein Families | Druggable Genome |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| Gene Summary | This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010] Transcript Variant: This variant (1) consists of 22 exons and encodes the shortest isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224149 | CARS (Myc-DDK-tagged)-Human cysteinyl-tRNA synthetase (CARS), transcript variant 1 |
CNY 5,320.00 |
|
| RC224149L3 | Lenti ORF clone of Human cysteinyl-tRNA synthetase (CARS), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC224149L4 | Lenti ORF clone of Human cysteinyl-tRNA synthetase (CARS), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG224149 | CARS (tGFP-tagged) - Human cysteinyl-tRNA synthetase (CARS), transcript variant 1 |
CNY 6,920.00 |
