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EEF1A1 (NM_001402) Human Untagged Clone
CAT＃: SC124558
EEF1A1 (untagged)-Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1)
CNY 3,656.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EF-Tu; EF1A; GRAF-1EF; LENG7; PTI1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124558 sequence for NM_001402 edited (data generated by NextGen Sequencing)
ATGGGAAAGGAAAAGACTCATATCAACATTGTCGTCATTGGACACGTAGATTCGGGCAAG TCCACCACTACTGGCCATCTGATCTATAAATGCGGTGGCATCGACAAAAGAACCATTGAA AAATTTGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTATGCCTGGGTCTTG GATAAACTGAAAGCTGAGCGTGAACGTGGTATCACCATTGATATCTCCTTGTGGAAATTT GAGACCAGCAAGTACTATGTGACTATCATTGATGCCCCAGGACACAGAGACTTTATCAAA AACATGATTACAGGGACATCTCAGGCTGACTGTGCTGTCCTGATTGTTGCTGCTGGTGTT GGTGAATTTGAAGCTGGTATCTCCAAGAATGGGCAGACCCGAGAGCATGCCCTTCTGGCT TACACACTGGGTGTGAAACAACTAATTGTCGGTGTTAACAAAATGGATTCCACTGAGCCA CCCTACAGCCAGAAGAGATATGAGGAAATTGTTAAGGAAGTCAGCACTTACATTAAGAAA ATTGGCTACAACCCCGACACAGTAGCATTTGTGCCAATTTCTGGTTGGAATGGTGACAAC ATGCTGGAGCCAAGTGCTAACATGCCTTGGTTCAAGGGATGGAAAGTCACCCGTAAGGAT GGCAATGCCAGTGGAACCACGCTGCTTGAGGCTCTGGACTGCATCCTACCACCAACTCGT CCAACTGACAAGCCCTTGCGCCTGCCTCTCCAGGATGTCTACAAAATTGGTGGTATTGGT ACTGTTCCTGTTGGCCGAGTGGAGACTGGTGTTCTCAAACCCGGTATGGTGGTCACCTTT GCTCCAGTCAACGTTACAACGGAAGTAAAATCTGTCGAAATGCACCATGAAGCTTTGAGT GAAGCTCTTCCTGGGGACAATGTGGGCTTCAATGTCAAGAATGTGTCTGTCAAGGATGTT CGTCGTGGCAACGTTGCTGGTGACAGCAAAAATGACCCACCAATGGAAGCAGCTGGCTTC ACTGCTCAGGTGATTATCCTGAACCATCCAGGCCAAATAAGCGCCGGCTATGCCCCTGTA TTGGATTGCCACACGGCTCACATTGCATGCAAGTTTGCTGAGCTGAAGGAAAAGATTGAT CGCCGTTCTGGTAAAAAGCTGGAAGATGGCCCTAAATTCTTGAAGTCTGGTGATGCTGCC ATTGTTGATATGGTTCCTGGCAAGCCCATGTGTGTTGAGAGCTTCTCAGACTATCCACCT TTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGGTGTCATCAAAGCA GTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAG GCTAAATGA Clone variation with respect to NM_001402.5 >OriGene 5' read for NM_001402 unedited
NTTCGGAATTGAAACCGATTACTATAGGCGGACGCCCAATTCGCACGAGGGCCGCCATAC ACAGGTGTCGTGAAACTACCCCCAAAGCCAAAAGGGAAAGGAAAAGACTCATATCAACAT TGTCGTCATTGGACACGTAGATTCGGGCAAGTCCACCACTACTGGCCATCTGATCTATAA ATGCGGTGGCATCGACAAAAGATTTTTTGTAAAAATTTGAGAAGGAGGCTGCTGAGATGG GAAAGGGCTCCTTCAAGTATGCCTGGGTCTTGGATAAACTGAAAGCTGAGCGTGAACGTG GTATCACCATTGATATCTCCTTGTGGAAATTTGAGACCAGCAAGTACTATGTGACTATCA TTGATGCCCCAGGACACAGAGACTTTATCAAAAACATGATTACAGGGACATCTCAGGCTG ACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGAAGCTGGTATCTCCAAGA ATGGGCAGACCCGAGAGCATGCCCTTCTGGCTTACACACTGGGTGTGAAACAACTAATTG TCGGTGTTAACAAAATGGATTCCACTGAGCCACCCTACAGCCAGAAGAGATATGAGGAAA TTGTTAAGGAAGTCAGCACTTACATTAAGAAAATTGGCTACAACCCCGACACAGTAGCAT TTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAGCCAAGTGCTAACATGCCTT GGTTCAAGGGATGGAAAGTCACCCGTAAAGATGGCCATGCCAGTGGAAACACGCCTGCTT GAGCTCTGGACTGCATCCTACCACCAACTCGTCCCACTGACAAGCCCCTTGCGCCCTGCC TCTCCAGGATGTCTACAAAATTGGTGGCNATTGGTACTGTTTCCTGTTGGNCCGAGTGAA AACTGCNTGTCTTCAA >OriGene 3' read for NM_001402 unedited
ATGGCAACTTTCCAGGNCCAGGNANAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGG ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT TTTTTCTCATTTAACTTTTTTAATGGGTCTCAAAATTCTGTGACAAATTTTTGGTCAAGT TGTTTCCATTAAAAAGTACTGATTTTAAAAACTAATAACTTAAAACTGCCACACGCAAAA AAGAAAACCAAAGTGGTCCACAAAACATTCTCCTTTCCTTCTGAAGGTTTTACGATGCAT TGTTATCATTAACCAGTCTTTTACTACTAAACTTAAATGGCCAATTGAAACAAACAGTTC TGAGACCGTTCTTCCACCACTGATTAAGAGTGGGGTGGCAGGTATTAGGGATAATATTCA TTTAGCCTTCTGAGCTTTCTGGGCAGACTTGGTGACCTTGCCAGCTCCAGCAGCCTTCTT GTCCACTGCTTTGATGACACCCACCGCAACTGTCTGTCTCATATCACGAACAGCAAAGCG ACCCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGNGGCTTGCCAGGAACCATAT CAACAATGGCAGCATCACCAGACTTCAAGAATTTAGGGCCATCTTCCAGCTTTTTACCAG AACGGCGATCAATCTTTTCCTTCAGCTCAGCAAACTTGCATGCAAATGTGAGCCGTGTGG CAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCANGATAATCACC TGAGCAGTGAAGCAGCTGCTCCATTGGTGGGTCATTTTTGCTGTCACCAGCACGTGCCAC GACGACATCCTTGCAGACACATTCTGACATGAGCCCACATTGTCCCAGNA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001402 |
| Insert Size | 1710 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001402.4, NP_001393.1 |
| RefSeq Size | 1837 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 1915 |
| UniProt ID | P68104 |
| Domains | GTP_EFTU, GTP_EFTU_D3, GTP_EFTU_D2 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Human eukaryotic elongation factor 1A forms oligomers through specific cysteine residues
,Liu, T;Yang, Y;Wang, D;Xiao, Y;Du, G;Wu, L;Ding, M;Li, L;Wu, C;,
Acta Biochim. Biophys. Sin. (Shanghai)
,PubMed ID 26515794
[EEF1A1]
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|
Use of phage display to identify novel mineralocorticoid receptor-interacting proteins
,Yang, J;Fuller, PJ;Morgan, J;Shibata, H;McDonnell, DP;Clyne, CD;Young, MJ;,
Mol. Endocrinol. July 2014
,PubMed ID 25000480
[EEF1A1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209697 | EEF1A1 (Myc-DDK-tagged)-Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) |
CNY 3,656.00 |
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| RC209697L3 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), Myc-DDK-tagged |
CNY 5,890.00 |
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| RC209697L4 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), mGFP tagged |
CNY 6,056.00 |
|
| RG209697 | EEF1A1 (tGFP-tagged) - Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) |
CNY 5,256.00 |
