United States
Germany
Japan
United Kingdom
China
MDA5 (IFIH1) (NM_022168) Human Untagged Clone
CAT＃: SC125308
IFIH1 (untagged)-Human interferon induced with helicase C domain 1 (IFIH1)
CNY 10,712.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2; SGMRT1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_022168, the custom clone sequence may differ by one or more nucleotides
ATGTCGAATGGGTATTCCACAGACGAGAATTTCCGCTATCTCATCTCGTGCTTCAGGGCCAGGGTGAAAA TGTACATCCAGGTGGAGCCTGTGCTGGACTACCTGACCTTTCTGCCTGCAGAGGTGAAGGAGCAGATTCA GAGGACAGTCGCCACCTCCGGGAACATGCAGGCAGTTGAACTGCTGCTGAGCACCTTGGAGAAGGGAGTC TGGCACCTTGGTTGGACTCGGGAATTCGTGGAGGCCCTCCGGAGAACCGGCAGCCCTCTGGCCGCCCGCT ACATGAACCCTGAGCTCACGGACTTGCCCTCTCCATCGTTTGAGAACGCTCATGATGAATATCTCCAACT GCTGAACCTCCTTCAGCCCACTCTGGTGGACAAGCTTCTAGTTAGAGACGTCTTGGATAAGTGCATGGAG GAGGAACTGTTGACAATTGAAGACAGAAACCGGATTGCTGCTGCAGAAAACAATGGAAATGAATCAGGTG TAAGAGAGCTACTAAAAAGGATTGTGCAGAAAGAAAACTGGTTCTCTGCATTTCTGAATGTTCTTCGTCA AACAGGAAACAATGAACTTGTCCAAGAGTTAACAGGCTCTGATTGCTCAGAAAGCAATGCAGAGATTGAG AATTTATCACAAGTTGATGGTCCTCAAGTGGAAGAGCAACTTCTTTCAACCACAGTTCAGCCAAATCTGG AGAAGGAGGTCTGGGGCATGGAGAATAACTCATCAGAATCATCTTTTGCAGATTCTTCTGTAGTTTCAGA ATCAGACACAAGTTTGGCAGAAGGAAGTGTCAGCTGCTTAGATGAAAGTCTTGGACATAACAGCAACATG GGCAGTGATTCAGGCACCATGGGAAGTGATTCAGATGAAGAGAATGTGGCAGCAAGAGCATCCCCGGAGC CAGAACTCCAGCTCAGGCCTTACCAAATGGAAGTTGCCCAGCCAGCCTTGGAAGGGAAGAATATCATCAT CTGCCTCCCTACAGGGAGTGGAAAAACCAGAGTGGCTGTTTACATTGCCAAGGATCACTTAGACAAGAAG AAAAAAGCATCTGAGCCTGGAAAAGTTATAGTTCTTGTCAATAAGGTACTGCTAGTTGAACAGCTCTTCC GCAAGGAGTTCCAACCATTTTTGAAGAAATGGTATCGTGTTATTGGATTAAGTGGTGATACCCAACTGAA AATATCATTTCCAGAAGTTGTCAAGTCCTGTGATATTATTATCAGTACAGCTCAAATCCTTGAAAACTCC CTCTTAAACTTGGAAAATGGAGAAGATGCTGGTGTTCAATTGTCAGACTTTTCCCTCATTATCATTGATG AATGTCATCACACCAACAAAGAAGCAGTGTATAATAACATCATGAGGCATTATTTGATGCAGAAGTTGAA AAACAATAGACTCAAGAAAGAAAACAAACCAGTGATTCCCCTTCCTCAGATACTGGGACTAACAGCTTCA CCTGGTGTTGGAGGGGCCACGAAGCAAGCCAAAGCTGAAGAACACATTTTAAAACTATGTGCCAATCTTG ATGCATTTACTATTAAAACTGTTAAAGAAAACCTTGATCAACTGAAAAACCAAATACAGGAGCCATGCAA GAAGTTTGCCATTGCAGATGCAACCAGAGAAGATCCATTTAAAGAGAAACTTCTAGAAATAATGACAAGG ATTCAAACTTATTGTCAAATGAGTCCAATGTCAGATTTTGGAACTCAACCCTATGAACAATGGGCCATTC AAATGGAAAAAAAAGCTGCAAAAGAAGGAAATCGCAAAGAACGTGTTTGTGCAGAACATTTGAGGAAGTA CAATGAGGCCCTACAAATTAATGACACAATTCGAATGATAGATGCGTATACTCATCTTGAAACTTTCTAT AATGAAGAGAAAGATAAGAAGTTTGCAGTCATAGAAGATGATAGTGATGAGGGTGGTGATGATGAGTATT GTGATGGTGATGAAGATGAGGATGATTTAAAGAAACCTTTGAAACTGGATGAAACAGATAGATTTCTCAT GACTTTATTTTTTGAAAACAATAAAATGTTGAAAAGGCTGGCTGAAAACCCAGAATATGAAAATGAAAAG CTGACCAAATTAAGAAATACCATAATGGAGCAATATACTAGGACTGAGGAATCAGCACGAGGAATAATCT TTACAAAAACACGACAGAGTGCATATGCGCTTTCCCAGTGGATTACTGAAAATGAAAAATTTGCTGAAGT AGGAGTCAAAGCCCACCATCTGATTGGAGCTGGACACAGCAGTGAGTTCAAACCCATGACACAGAATGAA CAAAAAGAAGTCATTAGTAAATTTCGCACTGGAAAAATAAATCTGCTTATCGCTACCACAGTGGCAGAAG AAGGTCTGGATATTAAAGAATGTAACATTGTTATCCGTTATGGTCTCGTCACCAATGAAATAGCCATGGT CCAGGCCCGTGGTCGAGCCAGAGCTGATGAGAGCACCTACGTCCTGGTTGCTCACAGTGGTTCAGGAGTT ATCGAACATGAGACAGTTAATGATTTCCGAGAGAAGATGATGTATAAAGCTATACATTGTGTTCAAAATA TGAAACCAGAGGAGTATGCTCATAAGATTTTGGAATTACAGATGCAAAGTATAATGGAAAAGAAAATGAA AACCAAGAGAAATATTGCCAAGCATTACAAGAATAACCCATCACTAATAACTTTCCTTTGCAAAAACTGC AGTGTGCTAGCCTGTTCTGGGGAAGATATCCATGTAATTGAGAAAATGCATCACGTCAATATGACCCCAG AATTCAAGGAACTTTACATTGTAAGAGAAAACAAAGCACTGCAAAAGAAGTGTGCCGACTATCAAATAAA TGGTGAAATCATCTGCAAATGTGGCCAGGCTTGGGGAACAATGATGGTGCACAAAGGCTTAGATTTGCCT TGTCTCAAAATAAGGAATTTTGTAGTGGTTTTCAAAAATAATTCAACAAAGAAACAATACAAAAAGTGGG TAGAATTACCTATCACATTTCCCAATCTTGACTATTCAGAATGCTGTTTATTTAGTGATGAGGATTAG >OriGene 5' read for NM_022168 unedited
TTGTAATCACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGACAGAAAGCTG GACTCAAAGCTCCTACCCGAGTGTGCAGCAGGATCGCCCCGGTCCGGGACCCCAGGCGCA CACCGCAGAGTCCAAAGTGCCGCGCCTGCCGGCCGCACCTGCCTGCCGCGGCCCCGCGCG CCGCCCCGCTGCCCACCTGCCCGCCTGCCCACCTGCCCAGGTGCGAGTGCAGCCCCGCGC GCCGGCCTGAGAGCCCTGTGGACAACCTCGTCATTGTCAGGCACAGAGCGGTAGACCCTG CTTCTCTAAGTGGGCAGCGGACAGCGGCACGCACATTTCACCTGTCCCGCAGACAACAGC ACCATCTGCTTGGGAGAACCCTCTCCCTTCTCTGAGAAAGAAAGATGTCGAATGGGTATT CCACAGACGAGAATTTCCGCTATCTCATCTCGTGCTTCAGGGCCAGGGTGAAAATGTACA TCCAGGTGGAGCCCTTGCTGGACTACCTGACCTTTCTGCCTGCAGAAGTGAAGGAGCATA TTCAGAGGACAGTCGCCACCTCCGGGAACATGCAGGCAGTTTGAACTGCTGCTGAACCCC TTGGAGAACGGAGTCTGGCACCTTGGGTCGCACTCGGGAATTCGTGGAGGCCCTCCCGAG AACCGGCAGNCCTTCTGCCCGCCCGCTACAATGAACCCTGAGCACACGGAACTTCCCCTT TCCATTGGTTTGAAAACCGCTCATGGATGATAATCTCCCAACTGGTGAAACCTCCCTTAA GCCCATTCTGGGAGGAACAAGCTTTTAATTTAAAAACGCCCTTGGATAAAGGGCATGCAA GGAAGGAACTGTTTGCAATTTGAAGAACAGAACCCGGTTTGCTGCGTGCGCAAACACCAT GGAATGATCCACGCGCCACAAATCCACTCACN >OriGene 3' read for NM_022168 unedited
GACCGCGGCCGCAATCTAGGATCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTGCAGAGTAAAACAATCATTTTATTGATTCTTATGGCAGTTCTGTACCCTAATGAAT ACCTTAATCATAATCCTATTAAATGGTTAACTGATAGCATTTTAAAAAAATCTTCAATCA AGGGCTAATCCTCATCACTAAATAAACAGCATTCTGAATAGTCAAAATTGGGAAATGGGA TAGGTAATTCTACCCACTTTTTGGATTGGTTCTTTGGTGAATTATTTTTGAAAACCCCCA CAAAATTCCTTATTTTGAAACAAGGCAAATCTAAGCCTTTGGGCACCATCATTGTTCCCC AACCCTGGCCACATTTGCCGATGATTTCACCATTTATTTGATAGCCGGCACACTTTTTTT GCAGTGGTTTGCTTTCTCTTACCATGTAAAGTTCCTTGAATTCTGGGGTCATATTGACCA GAAGCCTTTTCTCAATTACATGGATATCTTCCCCAGAACAGGCTAACACACTGCATTTTT TGCAAACGAAAGCTCTTACCCGATTACGGTATTCTTGTCACGCTCGGCAATATTTCTCTT GGCTTTCACTTTTTTTTCCATTATACTTTGCATCTGTTATTCCCAAACCTTATGAACCCA CTTCCCCTGGTTTCACCACTTTGAACCCACTGTTTACCTTCTACACATTACCCTTCTTTT CGGAAAACATTAAACTGTCATCCCCCTCCAAACCCCCTGAAACACTGTGCACAACCCAGA CCTAAGCGGCCCCCATACCCCCGGGCTTTACCCACGGCCCCCCCCATCGGTTTTCCCCTT GCGCCCCCCCCACCCCGGTCAAACCACGCTCCACTTTTCCTCACACCAACCTTTTCTCGT CCTTGGGGCGTTCGACAAACAAAACTTTTCCTTTCGCGGCCCAATTAACAGAGAGCTCCT TCTCGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022168 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022168.2, NP_071451.2 |
| RefSeq Size | 3434 bp |
| RefSeq ORF | 3078 bp |
| Locus ID | 64135 |
| UniProt ID | Q9BYX4 |
| Domains | DEAD, helicase_C |
| Protein Pathways | RIG-I-like receptor signaling pathway |
| Gene Summary | IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndrome[provided by RefSeq, Jul 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215661 | IFIH1 (Myc-DDK-tagged)-Human interferon induced with helicase C domain 1 (IFIH1) |
CNY 7,136.00 |
|
| RC215661L1 | Lenti ORF clone of Human interferon induced with helicase C domain 1 (IFIH1), Myc-DDK-tagged |
CNY 9,536.00 |
|
| RC215661L2 | Lenti ORF clone of Human interferon induced with helicase C domain 1 (IFIH1), mGFP tagged |
CNY 9,536.00 |
|
| RC215661L3 | Lenti ORF clone of Human interferon induced with helicase C domain 1 (IFIH1), Myc-DDK-tagged |
CNY 9,536.00 |
|
| RC215661L4 | Lenti ORF clone of Human interferon induced with helicase C domain 1 (IFIH1), mGFP tagged |
CNY 7,600.00 |
|
| RG215661 | IFIH1 (tGFP-tagged) - Human interferon induced with helicase C domain 1 (IFIH1) |
CNY 8,736.00 |
