COA1 (BC012036) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC125615 representing BC012036. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCACAGTGCAAAGAGGTGGCTGGTCAAGGACCAGTGGCATGGTGAAGTGTCTCCAGGGTGCACCGCT TTTGCGGCAGTTTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012036 unedited GTTCAGAATTTGTATACGACTCATATAGGCGGACCGCGATTCANATCTGGTACCGGTCCG GAATTCCCGGGATATCGTCGACCCACGCGTCCGAGAGAGGACATCATGNGAAAAAGTCAG ACTCTTTCTAGGATTTGAATCGGGAGTCTGAAGAGGAGAGGGGTGAATGTGCAAGCCCTT AACCACCCCACATCACTGATCTGGACCAGAGAACCTGCTTGGAAGAAGTGAGAAGCCATG CTAGCGAAGGCCAGGAGCTTGCAGGGGCTCAGAGCCTGGCAGTTGCATTAGTTTGCTGTG ATGCACAGTGCAAAGAGGTGGCTGGTCAAGGACCAGTGGCATGGTGAAGTGTCTCCAGGG TGCACCGCTTTTGCGGCAGTTTAGTATGGACAGAGTCTGGGCAGGCTCAGTGTAGCAAGC CTGTCATGAGAGGTGAGGACCAAGGTGGAGGTTGGTGTGACAGGAACAAGGAAAGGGAAG AACATAGAGCAGGAACACAGGAGAACAAAGAAAGGTCAGGACCGTCAGCCTCCAAAGGCC ACTGAGGAAGAGGGGCCATCCACTGGAATGGAAAACGAAGACGTACCTGTGGGGAAAGGA TCTTAAATTCAGTTTAACAGAGCAGGCCTTCAGCAAATACAAGAATTCTTAATTTCAGGT AGGAAGATATTAGCAGTGTCTTCTAATATTTGTTTTCGGAAAGATTTATGTCATTTTAGT TCACACAACTCTGTCTGCCAGCATCATTAACTTACCTAACTTGTTTAGAAACAANATGCT AACAGCACCACTGAGACCTTGCTAGTCTAAGTGTGCTTTGGAGACCATTGGCATCAGCAC CATTTANGCACTTGTTAGAATGTAGAGTCTCCGGGCAGTGGTGGTGGCTACACCTGTGAT CGACNNNTTTTTGGGGGGNNNNNNGNNGNNNNTTTTTNTTGGGGTT
Restriction Sites	SgfI-MluI
ACCN	BC012036
Insert Size	84 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC012036.1
RefSeq Size	1082 bp
RefSeq ORF	84 bp
Locus ID	55744
Protein Families	Transmembrane
MW	3 kDa
Gene Summary	Component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex, that regulates cytochrome c oxidase assembly. MITRAC complexes regulate both translation of mitochondrial encoded components and assembly of nuclear-encoded components imported in mitochondrion. Required for assembly of mitochondrial respiratory chain complex I and complex IV.[UniProtKB/Swiss-Prot Function]

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