C16orf57 (USB1) (BC006291) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ13154
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC006291 edited CTGGTGGACCTGCTCTGGTGGTCTTGGATGAGGCCCCATGAGCGCGGCGCCCCTGGTGGG CTACAGCAGCAGCGGCTCCGAGGATGAGTCCGAGGACGGGATGCGGACCAGGCCGGGGGA TGGGAGCCACCGTCGTGGCCAGAGCCCCCTTCCCAGGCAGAGATTTCCAGTACCTGACAG TGTGCTGAACATGTTCCCGGGCACCGAGGAGGGGCCTGAAGATGACAGCACAAAACACGG GGGACGGGTGCGCACCTTCCCCCACGAGCGAGGCAACTGGGCCACCCACGTCTATGTACC ATATGAAGCCAAGGAGGAGTTCCTGGATCTGCTTGATGTGTTGCTGCCCCATGCCCAGAC ATATGTCCCCCGGCTGGTAAGGATGAAGGTGTTCCACCTCAGCCTGTCCCAGAGTGTGGT TCTGCGCCACCACTGGATCCTCCCCTTCGTGCAGGCTCTGAAAGCCCGTATGACCTCCTT CCACAGATTCTTCTTTACTGCCAACCAGGTAAAGATTTACACCAATCAAGAGAAAACCAG GACCTTTATTGGGCTTGAGGTCACTTCAGGGCATGCCCAGTTCCTGGACCTGGTTTCAGA GGTGGACAGAGTCATGGAGGAATTCAACCTCACCACTTTCTACCAGGATCCTTCTTTCCA CCTCAGCCTGGCCTGGTGTGTGGGTGATGCACGTCTCCAGCTGGAGGGGCAGTGCCTGCA GGAACTACAGGCAATCGTGGATGGGTTTGAAGATGCTGAGGTGCTGCTGCGCGTGCACAC TGAGCAAGTCCGCTGCAAGTCTGGGAACAAGTTCTTCTCGATGCCTTTGAAGTGAGCACC AGAGGCCTTCCTCCTCCAGGGCCCTCTGCAGACCAGGCTGAGATGGAGGAACCTGCTAAA ATCGATGGAGATGCTTCTAGCCTCCCAGTAGGAGGCCCCAGCCATGCCTTCAACCTGGCA GGAGGTGTAGCCACTCCTCATCCTCCCTGAGTGCTGATATTCTCTCTCTCTCTTTCTCTT CCTCTTCTTTCTCTCTCTTCTCCTCTCTTTCTCTCCTCTGTCTCTCTTCCTCTCCTCTCT TCCTCTCTTCTCTCTTCCTCTCCTCTCTCTCTCTTCCTCTCCTCTCTCTACCCCTCCTGT CTCTCCTCCCCTCCTCTCTCTTCCTCTCCTCTCTCTCTTCCTCTCCTCTCTCTTCCCTTC CTGTCTCTCTTCCCCTCCTCTCTCTCTTCCTGTCCTCTATCTCTTCCCCTCCTCTATCTC TTCCTCTCCTCTCTCTCTTCCTCTCCTCTCTCTCTCTTCCTTTCTTCTCTCTCTCCTGTC TCGGCTGTTGTGGGTTGCAGGTTGGGTGCTGCTGTTGTGGTCCTTCCCAGAAACTGCCAG TAGAGGGCAGCCTGGGCATCCTAATGCTTACTCTGGTTGTTACACAAAGAAAATATTGGG GTCACTGGCGAGCCCACCCACACTCACCAGAATCTCCACTGTAGTCCCCCTAACAAACAG CCCTTCACTTCCTCTTCCACTTCAGCAATTTGTATTTTGATGCCATTGGCCTCAGATCAG AGTGTTTTAAATCATCACGCCCTGGCTTATCCCTGGTCGAGCCAGGACACGGGGTGCTTC AGTGGGTCTGTCACCCTCTCTCCTTGAAGCATGTTGCTTTTATTTATTTACTTTTACTCT CACCCTGCTCCTGTACCAGCAGGGGCCACTTCAAAGCCAAGGTACAGGGTGATAACTTGT GGTCCAGCATCAGTTTTCTCCACTTCTTTCTCCCACTCACCCCCAGCAAGGTGCCTGGGG AGACTTGAGCAGATGTTTCATTTTGGCCTGGCCAGTGGCTGAAAGCCAGGCCTCCAATGC ACTGTGACCTCTGGCTTCCCCAGCAGCTTTCCCAGAGAGGCAGAGGGGCCTTCCACAGCC CGGGTTCTCCTGCTGCCTCCTGCCTGCTGCAGCTGCAGGCATTCTGAGGGGCAACGTGGA GGAAGGGCCAGGGATGCATGGGATTTTAATTGTTTCATCACACCTTCCCCGTGGCAAAGA AACAGTCAGTCCTCTTCAGGTGTCTTCTGGATTTCTGGTGATGGACAGAGAAATCTTTTT ACAGTTTCAAATTATGTTCAACAAATAAAAATTGCATTTTTTAAAAAAAAAAAAAAAAAA A 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC006291 unedited GGGAGTTAGATTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCTGGTG GACCTGCTCTGGTGGTCTTGGATGAGGCCCCATGAGCGCGGCGCCCCTGGTGGGCTACAG CAGCAGCGGCTCCGAGGATGAGTCCGAGGACGGGATGCGGACCAGGCCGGGGGATGGGAG CCACCGTCGTGGCCAGAGCCCCCTTCCCAGGCAGAGATTTCCAGTACCTGACAGTGTGCT GAACATGTTCCCGGGCACCGAGGAGGGGCCTGAAGATGACAGCACAAAACACGGGGGACG GGTGCGCACCTTCCCCCACGAGCGAGGCAACTGGGCCACCCACGTCTATGTACCATATGA AGCCAAGGAGGAGTTCCTGGATCTGCTTGATGTGTTGCTGCCCCATGCCCAGACATATGT CCCCCGGCTGGTAAGGATGAAGGTGTTCCACCTCAGCCTGTCCCAGAGTGTGGTTCTGCG CCACCACTGGATCCTCCCCTTCGTGCAGGCTCTGAAAGCCCGTATGACCTCCTTCCACAG ATTCTTCTTTACTGCCAACCAGGTAAAGATTTACACCAATCAAGAGAAAACCAGGACCTT TATTGGGCTTGAGGTCACTTCAGGGCATGCCCAGTTCCTGGACCTGGTTTCAGAGGTGGA CAGAGTCATGGAGGAATTCAACCTCACCACTTTCTACCAGGATCCTTCTTTCCACCTCAG CCTGGCCTGGTGTGTGGGTGATGCACGTCTCCAGCTGGAGGGGCAGTGCCCTGCAGGACT ACAGGCCATCGTGGATGGGTTTTGAAGATGCTGAGGTGCTGCTGCGCGTGCACACTGAGC AAGTCCGCTTGCAGTCTGGGGAACAATTTCTCTTAGATGCCTTTGAAGTGAGCACCAAG
Restriction Sites	Please inquire
ACCN	BC006291
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006291.2, AAH06291.1
RefSeq Size	2161 bp
Locus ID	79650
Gene Summary	This gene encodes a protein with several conserved domains, however, its exact function is not known. Mutations in this gene are associated with poikiloderma with neutropenia (PN), which shows phenotypic overlap with Rothmund-Thomson syndrome (RTS) caused by mutations in the RECQL4 gene. It is believed that this gene product interacts with RECQL4 protein via SMAD4 proteins, explaining the partial clinical overlap between PN and RTS. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]

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