SCAMP4 (BC011747) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ33847; FLJ90105; SCAMP-4; secretory carrier membrane protein 4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC011747 edited GCGCTGCGCTCGCGCCCGGATCCCTCAGGCGGCTGCAGGCTTCAGCCTGCGCTGGTTGGT GAAACAGAGATGTCAGAAAAGGAGAACAACTTCCCGCCACTGCCCAAGTTCATCCCTGTG AAGCCCTGCTTCTACCAGAACTTCTCCGACGAGATCCCAGTGGAGCACCAGGTCCTGGTG AAGAGGATCTACCGGCTGTGGATGTTTTACTGCGCCACCCTCGGCGTCAACCTCATTGCC TGCCTGGCCTGGTGGATCGGCGGAGGCTCGGGGACCAACTTCGGCCTGGCCTTCGTGTGG CTGCTCCTGTTCACGCCTTGCGGCTACGTGTGCTGGTTCCGGCCTGTCTACAAGGCCTTC CGAGCCGACAGCTCCTTTAATTTCATGGCGTTTTTCTTCATCTTCGGAGCCCAGTTTGTC CTGACCGTCATCCAGGCGATTGGCTTCTCCGGCTGGGGCGCGTGCGGCTGGCTGTCGGCA ATTGGATTCTTCCAGTACAGCCCGGGCGCTGCCGTGGTCATGCTGCTTCCAGCCATCATG TTCTCCGTGTCGGCTGCCATGATGGCCATCGCGATCATGAAGGTGCACAGGATCTACCGA GGGGCTGGCGGAAGCTTCCAGAAGGCACAGACGGAGTGGAACACGGGCACTTGGCGGAAC CCACCGTCGAGGGAGGCCCAGTACAACAACTTCTCAGGCAACAGCCTGCCCGAGTACCCC ACTGTGCCCAGCTACCCGGGCAGTGGCCAGTGGCCTTAGAGGGAGCCTGCCCTGCCCCCA CCGCCCACCACCTCCTCCCCTTCATTCCTGCTGCTACCCCTGGTCCCGAGGGCTGGGAGT ACCTGGGGCCCCATCCCCCCAGCTGGGATGGTGGAAGCCGGTGGTGGCCACGGACCGCCC CCCTCCTGCCAGGGCCACAGAACCCGTGTTCATCTCATCCGAGAGCGGAGTTCCTCACAA GCACTCCCCAGCAGCCCTTGGCCTCTGCCGTCCACAGGACGCCCTCTTGCTCCCGGAAAC GTGTGGTCACCCGCCGTCCACTGCACGGCTGGTACGGCCTTGTCTTCAGGTCTCGAGGCC TGACTCCGGGGGACAGGTGGCAGCAGGTCGGCCGCCCTCCCGTCCTCCAAGAGCTGCTGG CGCTGAGGTCAGAGCGGGTCTGATGGGGAGCTCCGTCTCACCGGCCACCCGCCGTCACCA TGGCAGATGCCCTTGGCCGGAACTAATAAGAGGCGTCGGGGCCAGCTTCCGGTCCCCTGC AGTGATAGAGGGCTTGGTGCCTAGCTGAGTCCTCGCTGTCCCCGCCATCCCCTGATCTGT GCGGCTCCAGCCTCGCCCCCTCCCCACGTGCACCATACCTGGGGAGTTCCTGGTCCAGGG TATCCTGGGGCCACCCTCCCTGCCTCCAAAACAGGGATCCCTGGCAGGCTGTCTTTCCAC GCCCCTGAGTTCAGAGTCGGGGACCCAGGCCAGGTCGGGAGCACAGCCGCTCCCCAAACC CAGCAAACCGGCAGAGAGCCGGTTTCCCAGCAGCCGGAGCCCTGCAGGAGAGGCCTTTGT GTTTTGTTTTGTTTTGTTTTTTCTCTTTTGAGACAGAGTTTCACTCTGTCGCCCAGGCTG GAGTGCAGTGGTGTGATCTCGGCTCACTGCAACCTCTGCCTCCCGTGTTCAAGCAGTTCT CCTGCCTCAGCCTCCCAAATAGCTGGGATTACAGTTGCCTGCCACCACGCCCAGCTAATT TTTATATTTTTAGTACAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTG ACCTCAAGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATAATAGGTGTCAGCCAC CGCGCCCAGCCTGGAGTGGCCTTTTATGAGAGGGGACCCGTCAAATCTGTGCCTTATGGA GGGGTCCGGCAGCGGCCACAATTGTCTTGTCCCCTCACCCCCCAACTCCCCCTGGAACAC CTCTCCCAGGCAAGACATTTTCACAGCACCATTCACAACGGTTGGGCCAAAAAGAAACTT TTCCTTCATCATTTCCTGCACTCGCTGACCACAACTTTGGACACCCCAGGCTGCCACCCC TCCCCACCCGTTCACCCCCAGGATGCTGTTGCTGTAGGACGCCTGCTGCCCTGGAGCCCT CCCCAGGATGTGAGCCAGTCCCCTCGCTGGTACGGAATGCCGCTGGGTGCCCGGAGGCGG CCATGGTGTCTCGATGGACGGCAGCCAGGATGGAGCACCCATGGGTCTCACGGCCATGCT TCAGGGTCTTCAGGTCCTGCCCCCGGCCAGTCTGCCAAGAGGCACCCCCTTCCCCAGCCT CTCGCCTGCACTGATGCAGACAAAATCTCACCTGGCAGGCCCAACCCCCCCCACCCCTCC CCCGCCGTGTGTGGCTCCTCGCCGCATCGTTGGGGTTTTGTTATGTGAAAATATCCTGGA AATAAATACATGTTTCTGCACTTAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011747 unedited NGTGAAGTCAGACTTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCGC TGCGCTCGCGCCCGGCTCCCTCAGGCGGCTGCAGGCTTCAGCCTGCGCTGGTTGGTGAAA CAGAGATGTCAGAAAAGGAGAACAACTTCCCGCCACTGCCCAAGTTCATCCCTGTGAAGC CCTGCTTCTACCAGAACTTCTCCGACGAGATCCCAGTGGAGCACCAGGTCCTGGTGAAGA GGATCTACCGGCTGTGGATGTTTTACTGCGCCACCCTCGGCGTCAACCTCATTGCCTGCC TGGCCTGGTGGATCGGCGGAGGCTCGGGGACCAACTTCGGCCTGGCCTTCGTGTGGCTGC TCCTGTTCACGCCTTGCGGCTACGTGTGCTGGTTCCGGCCTGTCTACAAGGCCTTCCGAG CCGACAGCTCCTTTAATTTCATGGCGTTTTTCTTCATCTTCGGAGCCCAGTTTGTCCTGA CCGTCATCCAGGCGATTGGCTTCTCCGGCTGGGGCGCGTGCGGCTGGCTGTCGGCAATTG GATTCTTCCAGTACAGCCCGGGCGCTGCCGTGGTCATGCTGCTTCCAGCCATCATGTTCT CCGTGTCGGCTGCCATGATGGCCATCGCGATCATGAAGGTGCACAGGATCTACCGAGGGG CTGGCGGAAGCTTCCAGAAGGCACAGACGGAGTGGAACACGGGCACTTGGCGGAACCCAC CGTCGAGGGAGGCCCAGTACAACAACTTCTCAGGCAACAGCCTGCCCGAGTACCCCACTG TGCCCAGCTACCCGGGCAGTGGCCAGTGGCTTTAGAGGAGCCTGCCCTGCCCCACCGCCA CACCTCCTCCCTTTATTCTGCTGCTACCCTGGTCCCGAGGGCGG
Restriction Sites	Please inquire
ACCN	BC011747
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011747.2, AAH11747.1
RefSeq Size	2503 bp
Locus ID	113178
Gene Summary	Secretory carrier membrane proteins (SCAMPs) are widely distributed integral membrane proteins implicated in membrane trafficking. Most SCAMPs (e.g., SCAMP1; MIM 606911) have N-terminal cytoplasmic NPF (arg-pro-phe) repeats, 4 central transmembrane regions, and a short C-terminal cytoplasmic tail. These SCAMPs likely have a role in endocytosis that is mediated by their NPF repeats. Other SCAMPs, such as SCAMP4, lack the NPF repeats and are therefore unlikely to function in endocytosis (summary by Fernandez-Chacon and Sudhof, 2000 [PubMed 11050114]).[supplied by OMIM, Feb 2011]

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