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GLI1 (NM_005269) Human Untagged Clone
CAT＃: SC125780
GLI1 (untagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 1
CNY 11,560.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GLI; PAPA8; PPD1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005269 edited
CCCAGACAGAGTGTCCCCACACCCTCCTCTGAGACGCCATGTTCAACTCGATGACCCCAC CACCAATCAGTAGCTATGGCGAGCCCTGCTGTCTCCGGCCCCTCCCCAGTCAGGGGGCCC CCAGTGTGGGGACAGAAGGACTGTCTGGCCCGCCCTTCTGCCACCAAGCTAACCTCATGT CCGGCCCCCACAGTTATGGGCCAGCCAGAGAGACCAACAGCTGCACCGAGGGCCCACTCT TTTCTTCTCCCCGGAGTGCAGTCAAGTTGACCAAGAAGCGGGCACTGTCCATCTCACCTC TGTCGGATGCCAGCCTGGACCTGCAGACGGTTATCCGCACCTCACCCAGCTCCCTCGTAG CTTTCATCAACTCGCGATGCACATCTCCAGGAGGCTCCTACGGTCATCTCTCCATTGGCA CCATGAGCCCATCTCTGGGATTCCCAGCCCAGATGAATCACCAAAAAGGGCCCTCGCCTT CCTTTGGGGTCCAGCCTTGTGGTCCCCATGACTCTGCCCGGGGTGGGATGATCCCACATC CTCAGTCCCGGGGACCCTTCCCAACTTGCCAGCTGAAGTCTGAGCTGGACATGCTGGTTG GCAAGTGCCGGGAGGAACCCTTGGAAGGTGATATGTCCAGCCCCAACTCCACAGGCATAC AGGATCCCCTGTTGGGGATGCTGGATGGGCGGGAGGACCTCGAGAGAGAGGAGAAGCGTG AGCCTGAATCTGTGTATGAAACTGACTGCCGTTGGGATGGCTGCAGCCAGGAATTTGACT CCCAAGAGCAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGGGAGCGGAAGGAGT TCGTGTGCCACTGGGGGGGCTGCTCCAGGGAGCTGAGGCCCTTCAAAGCCCAGTACATGC TGGTGGTTCACATGCGCAGACACACTGGCGAGAAGCCACACAAGTGCACGTTTGAAGGGT GCCGGAAGTCATACTCACGCCTCGAAAACCTGAAGACGCACCTGCGGTCACACACGGGTG AGAAGCCATACATGTGTGAGCACGAGGGCTGCAGTAAAGCCTTCAGCAATGCCAGTGACC GAGCCAAGCACCAGAATCGGACCCATTCCAATGAGAAGCCGTATGTATGTAAGCTCCCTG GCTGCACCAAACGCTATACAGATCCTAGCTCGCTGCGAAAACATGTCAAGACAGTGCATG GTCCTGACGCCCATGTGACCAAACGGCACCGTGGGGATGGCCCCCTGCCTCGGGCACCAT CCATTTCTACAGTGGAGCCCAAGAGGGAGCGGGAAGGAGGTCCCATCAGGGAGGAAAGCA GACTGACTGTGCCAGAGGGTGCCATGAAGCCACAGCCAAGCCCTGGGGCCCAGTCATCCT GCAGCAGTGACCACTCCCCGGCAGGGAGTGCAGCCAATACAGACAGTGGTGTGGAAATGA CTGGCAATGCAGGGGGCAGCACTGAAGACCTCTCCAGCTTGGACGAGGGACCTTGCATTG CTGGCACTGGTCTGTCCACTCTTCGCCGCCTTGAGAACCTCAGGCTGGACCAGCTACATC AACTCCGGCCAATAGGGACCCGGGGTCTCAAACTGCCCAGCTTGTCCCACACCGGTACCA CTGTGTCCCGCCGCGTGGGCCCCCCAGTCTCTCTTGAACGCCGCAGCAGCAGCTCCAGCA GCATCAGCTCTGCCTATACTGTCAGCCGCCGCTCCTCCCTGGCCTCTCCTTTCCCCCCTG GCTCCCCACCAGAGAATGGAGCATCCTCCCTGCCTGGCCTTATGCCTGCCCAGCACTACC TGCTTCGGGCAAGATATGCTTCAGCCAGAGGGGGTGGTACTTCGCCCACTGCAGCATCCA GCCTGGATCGGATAGGTGGTCTTCCCATGCCTCCTTGGAGAAGCCGAGCCGAGTATCCAG GATACAACCCCAATGCAGGGGTCACCCGGAGGGCCAGTGACCCAGCCCAGGCTGCTGACC GTCCTGCTCCAGCTAGAGTCCAGAGGTTCAAGAGCCTGGGCTGTGTCCATACCCCACCCA CTGTGGCAGGGGGAGGACAGAACTTTGATCCTTACCTCCCAACCTCTGTCTACTCACCAC AGCCCCCCAGCATCACTGAGAATGCTGCCATGGATGCTAGAGGGCTACAGGAAGAGCCAG AAGTTGGGACCTCCATGGTGGGCAGTGGTCTGAACCCCTATATGGACTTCCCACCTACTG ATACTCTGGGATATGGGGGACCTGAAGGGGCAGCAGCTGAGCCTTATGGAGCGAGGGGTC CAGGCTCTCTGCCTCTTGGGCCTGGTCCACCCACCAACTATGGCCCCAACCCCTGTCCCC AGCAGGCCTCATATCCTGACCCCACCCAAGAAACATGGGGTGAGTTCCCTTCCCACTCTG GGCTGTACCCAGGCCCCAAGGCTCTAGGTGGAACCTACAGCCAGTGTCCTCGACTTGAAC ATTATGGACAAGTGCAAGTCAAGCCAGAACAGGGGTGCCCAGTGGGGTCTGACTCCACAG GACTGGCACCCTGCCTCAATGCCCACCCCAGTGAGGGGCCCCCACATCCACAGCCTCTCT TTTCCCATTACCCCCAGCCCTCTCCTCCCCAATATCTCCAGTCAGGCCCCTATACCCAGC CACCCCCTGATTATCTTCCTTCAGAACCCAGGCCTTGCCTGGACTTTGATTCCCCCACCC ATTCCACAGGGCAGCTCAAGGCTCAGCTTGTGTGTAATTATGTTCAATCTCAACAGGAGC TACTGTGGGAGGGTGGGGGCAGGGAAGATGCCCCCGCCCAGGAACCTTCCTACCAGAGTC CCAAGTTTCTGGGGGGTTCCCAGGTTAGCCCAAGCCGTGCTAAAGCTCCAGTGAACACAT ATGGACCTGGCTTTGGACCCAACTTGCCCAATCACAAGTCAGGTTCCTATCCCACCCCTT CACCATGCCATGAAAATTTTGTAGTGGGGGCAAATAGGGCTTCACATAGGGCAGCAGCAC CACCTCGACTTCTGCCCCCATTGCCCACTTGCTATGGGCCTCTCAAAGTGGGAGGCACAA ACCCCAGCTGTGGTCATCCTGAGGTGGGCAGGCTAGGAGGGGGTCCTGCCTTGTACCCTC CTCCCGAAGGACAGGTATGTAACCCCCTGGACTCTCTTGATCTTGACAACACTCAGCTGG ACTTTGTGGCTATTCTGGATGAGCCCCAGGGGCTGAGTCCTCCTCCTTCCCATGATCAGC GGGGCAGCTCTGGACATACCCCACCTCCCTCTGGGCCCCCCAACATGGCTGTGGGCAACA TGAGTGTCTTACTGAGATCCCTACCTGGGGAAACAGAATTCCTCAACTCTAGTGCCTAAA GAGTAGGGAATCTCATCCATCACAGATCGCATTTCCTAAGGGGTTTCTATCCTTCCAGAA AAATTGGGGGAGCTGCAGTCCCCTGCACAAGATGCCCCAGGGATGGGAGGTATGGGCTGG GGGCTATGTATAGTCTGTATACGTTTTGAGGAGAAATTTGATAATGACACTGTTTCCTGA TAATAAAGGAACTGCATCAGAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_005269 unedited
NNGGGTTCAAAATTGTATACGACTCATATAGGCGGACCGCGAATTCGCACGAGGCCCAGA CAGAGTGTCCCCACACCCTCCTCTGAGACGCCATGTTCAACTCGATGACCCCACCACCAA TCAGTAGCTATGGCGAGCCCTGCTGTCTCCGGCCCCTCCCCAGTCAGGGGGCCCCCAGTG TGGGGACAGAAGGACTGTCTGGCCCGCCCTTCTGCCACCAAGCTAACCTCATGTCCGGCC CCCACAGTTATGGGCCAGCCAGAGAGACCAACAGCTGCACCGAGGGCCCACTCTTTTCTT CTCCCCGGAGTGCAGTCAAGTTGACCAAGAAGCGGGCACTGTCCATCTCACCTCTGTCGG ATGCCAGCCTGGACCTGCAGACGGTTATCCGCACCTCACCCAGCTCCCTCGTAGCTTTCA TCAACTCGCGATGCACATCTCCAGGAGGCTCCTACGGTCATCTCTCCATTGGCACCATGA GCCCATCTCTGGGATTCCCAGCCCAGATGAATCACCAAAAAGGGCCCTCGCCTTCCTTTG GGGTCCAGCCTTGTGGTCCCCATGACTCTGCCCGGGGTGGGATGATCCCACATCCTCAGT CCCGGGGACCCTTCCCAACTTGCCAGCTGAAGTCTGAGCTGGACATGCTGGTTGGCAAGT GCCGGGAGGAACCCTTGGAAGGTGATATGTCCAGCCCCAACTCCACAGGCATACAGGATC CCCTGTTGNGGATGCTGGATGGGCGGGAGGACCTCGAGAGAGAGGAAAGCGTGAGCCTGA ATCTGTGTATGAAACTGACTGCCGTTGGGATGGCTGCAGCCAGGAATTTGACTCCCAAGA CAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGNGAGCGGAAGGAGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005269 |
| Insert Size | 3600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005269.1, NP_005260.1 |
| RefSeq Size | 3600 bp |
| RefSeq ORF | 3321 bp |
| Locus ID | 2735 |
| UniProt ID | P08151 |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| Gene Summary | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] Transcript Variant: This variant (1) encodes the full length protein (isoform 1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ERK2 MAP kinase regulates SUFU binding by multisite phosphorylation of GLI1
,null,
Life Science Alliance
,PubMed ID 35831023
[GLI1]
|
|
Forkhead box M1B is a determinant of rat susceptibility to hepatocarcinogenesis and sustains ERK activity in human HCC
,D F Calvisi, F Pinna, S Ladu, R Pellegrino, M M Simile, M Frau, M R De Miglio, M L Tomasi, V Sanna, M R Muroni, F Feo, and R M Pascale,
Gut, May 2009; 58: 679 - 687.
[GLI1]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201110 | GLI1 (Myc-DDK-tagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 1 |
CNY 11,552.00 |
|
| RC201110L1 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, Myc-DDK-tagged |
CNY 13,952.00 |
|
| RC201110L2 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, mGFP tagged |
CNY 11,400.00 |
|
| RC201110L3 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, Myc-DDK-tagged |
CNY 13,952.00 |
|
| RC201110L4 | Lenti ORF clone of Human GLI family zinc finger 1 (GLI1), transcript variant 1, mGFP tagged |
CNY 13,952.00 |
|
| RG201110 | GLI1 (tGFP-tagged) - Human GLI family zinc finger 1 (GLI1), transcript variant 1 |
CNY 13,152.00 |
