C13orf18 (RUBCNL) (BC043488) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	chromosome 13 open reading frame 18; FLJ21562
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC043488 edited GGAGCGCGGTGGCCACGGAACGCTGCCCGGAGCCGCGCGAGGGAGGACCCGACGCGCGGC GTTTACCCAGCGCAGCGTTCCACCGCTCGGGTTTGGCTGGATAAAATAAAAAATGGGGAT ATTGACCTCCTGTCACTACTGCATGGACTTTGATGGTTTCCAATCATTACTTTCTCCTCT GTGTCAATCTGCCTCTTCGAGAAATTCATACTCCTGGCCCTTCACCTTCGTGCCTCGGGG ACTCCCTGGCAGAGACAACGTTGTCTGAGGATACCACAGACTCCGTTGGCAGCGCTTCTC CCCATGGCTCGAGTGAAAAGAGTAGCAGCTTCTCTCTGTCCTCAACAGAGGTACACATGG TCCGCCCAGGATACTCTCATCGGGTGTCTCTGCCCACAAGCCCTAGGATTTTGGCCACCT CCCCATATCCTGAGACTGACAGTGCTTTTTTTGAGCCTTCCCATCTGACATCTGCTGCTG ATGAAGGTGCTGTTCAAGTCAGTAGAAGAACCATTTCTTCGAATTCCTTCTCACCAGAGG TATTTGTGCTGCCTGTTGATGTAGAAAAGGAAAATGCCCACTTTTATGTTGCAGATATGA TTATATCAGCAATGGAGAAAATGAAGTGTAACATTCTGAGTCAACAGCAGACAGAGAGCT GGAGTAAAGAAGTCAGTGGGTTACTTGGGAGTGATCAGCCTGACTCTGAAATGACTTTTG ATACCAACATAAAGCAAGAGTCTGGGTCTTCTACTTCTTCATACAGTGGCTATGAAGGTT GTGCTGTGTTACAGGTCAGCCCAGTGACTGAAACACGTACTTACCATGATGTGAAAGAGA TTTGCAAATGCGATGTTGATGAATTTGTTATTTTAGAGCTTGGAGATTTTAATGATATCA CAGAAACCTGTAGCTGTTCCTGCAGCTCCTCTAAGAGTGTCACTTATGAGCCAGACTTCA ATTCTGCAGAACTATTAGCCAAAGAGCTGTACCGCGTGTTCCAGAAGTGCTGGATACTGT CAGTAGTTAATTCTCAGCTGGCAGGTTCCCTGAGTGCAGCTGGCTCGATAGTCGTAAATG AAGAGTGTGTCCGAAAAGACTTTGAATCCAGTATGAATGTAGTACAGGAAATTAAATTTA AGTCTAGGATCAGAGGGACTGAAGACTGGGCTCCTCCTAGATTTCAAATCATATTTAATA TTCATCCACCACTCAAGAGGGACCTTGTGGTGGCAGCCCAGAATTTTTTCTGTGCCGGCT GTGGAACTCCAGTAGAGCCTAAGTTTGTGAAGCGGCTCCGGTACTGCGAATACCTAGGGA AGTATTTCTGTGACTGCTGCCACTCATATGCAGAGTCGTGCATCCCTGCCCGAATCCTGA TGATGTGGGACTTCAAGAAGTACTACGTCAGCAATTTCTCCAAACAGCTGCTCGACAGCA TATGGCACCAGCCCATTTTCAATTTGCTGAGCATCGGCCAAAGCCTGTATGCGAAAGCCA AGGAGCTGGACAGAGTGAAGGAAATTCAGGAGCAGCTCTTCCATATCAAGAAGCTGTTGA AGACCTGTAGGTTTGCTAACAGTGCATTAAAGGAGTTCGAGCAGGTGCCGGGACACTTGA CTGATGAGCTCCACCTGTTCTCCCTTGAGGACCTGGTCAGGATCAAGAAAGGGCTGCTGG CACCCTTACTCAAGGACATTCTGAAAGCTTCCCTTGCACATGTGGCTGGCTGTGAGCTGT GTCAAGGAAAGGGCTTTATTTGTGAATTTTGCCAGAATACGACTGTCATCTTCCCATTTC AGACAGCAACATGTAGAAGATGTTCAGCGTGCAGGGCTTGCTTTCACAAACAGTGCTTCC AGTCCTCCGAGTGCCCCCGGTGTGCGAGGATCACAGCGAGGAGAAAACTTCTGGAAAGTG TGGCCTCTGCAGCAACATGATGCCCCTGAGTACTGTGAAAAAGACTGTTCAACATGCCTT ATGATAACACCGATTTGTGTCTATTATTGGTGACATTGTTTTAGATATTGGGTATTGTAT ATTAAGGAAAAAGATGGTCTATATTCTCTTTATTGCATATACTTAATGTTTCAAAAGAAT GCAGATTCTGTGTTTAAGCACAGGGCTGATAGTTGTGGTTTTGTTTACAAATGTTCTGTT TTGGCTGCTATTGGTTTTTTAAAGAGGTTTTTTATACTTTTGTATTTGAATAGTTATGTT TCACTGATGCTGAGCCAGTTTGTATGTGTGTGCATATATGTGAACTGTAACTGACAAGAT GAATTACTCAGTTTCTCTTTCTCTAAAGCTTGTTTGATGAAACTGGTTGGTCCTTTCAGT GAACAAAAATATGACCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC043488 unedited NNNCCCGGGCAGAATTTTGTATACGACTCATATAGGCGGCCGCGNATTCAGATCTGGTAC CGGTCCGGAATTCCCGGGATGGAGCGCGGTGGCCACGGAACGCTGCCCGGAGCCGCGCGA GGGAGGACCCGACGCGCGGCGTTTACCCAGCGCAGCGTTCCACCGCTCGGGTTTGGCTGG ATAAAATAAAAAATGGGGATATTGACCTCCTGTCACTACTGCATGGACTTTGATGGTTTC CAATCATTACTTTCTCCTCTGTGTCAATCTGCCTCTTCGAGAAATTCATACTCCTGGCCC TTCACCTTCGTGCCTCGGGGACTCCCTGGCAGAGACAACGTTGTCTGAGGATACCACAGA CTCCGTTGGCAGCGCTTCTCCCCATGGCTCGAGTGAAAAGAGTAGCAGCTTCTCTCTGTC CTCAACAGAGGTACACATGGTCCGCCCAGGATACTCTCATCGGGTGTCTCTGCCCACAAG CCCTAGGATTTTGGCCACCTCCCCATATCCTGAGACTGACAGTGCTTTTTTTGAGCCTTC CCATCTGACATCTGCTGCTGATGAAGGTGCTGTTCAAGTCAGTAGAAGAACCATTTCTTC GAATTCCTTCTCACCAGAGGTATTTGTGCTGCCTGTTGATGTAGAAAAGGAAAATGCCCA CTTTTATGTTGCAGATATGATTATATCAGCAATGGAGAAAATGAAGTGTAACATTCTGAG TCAACAGCAGACAGAGAGCTGGAGTAAAGAAGTCAGTGGGTTACTTGGGAGTGATCAGCC TGACTCTGAAATGACTTTTGATACCAACATAAAAGCAGAGTCTGGGTCTTCTACTTTCTC ATACAGTGGCTATGAAAGTTGTGCTGTGTTC
Restriction Sites	NotI-NotI
ACCN	BC043488
Insert Size	2400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC043488.1, AAH43488.1
RefSeq Size	2422 bp
Locus ID	80183
Gene Summary	This gene encodes a cysteine-rich protein that contains a putative zinc-RING and/or ribbon domain. The encoded protein is related to Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, which plays a role in endocytic trafficking and autophagy. In cervical cancer cell lines, this gene is expressed at low levels and may function as a tumor suppressor. Promoter hypermethylation of this gene is observed in cervical cancer cell lines and tissue derived from human patients. [provided by RefSeq, Mar 2017]

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